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AB231239

Anti-KMT2D / MLL2 antibody

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(1 Publication)

Rabbit Polyclonal KMT2D / MLL2 antibody. Suitable for WB and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Mouse Kmt2d conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Mll2, Mll4, Kmt2d, Histone-lysine N-methyltransferase 2D, Lysine N-methyltransferase 2D, ALL1-related protein, Myeloid/lymphoid or mixed-lineage leukemia protein 2

1 Images
Western blot - Anti-KMT2D / MLL2 antibody (AB231239)
  • WB

Supplier Data

Western blot - Anti-KMT2D / MLL2 antibody (AB231239)

Cells homozygous for the targeted KMT2D / MLL2 allele (-/-) show a complete loss of KMT2D / MLL2 protein, whereas the KMT2D / MLL2 protein was detected in fp-recombined ES cells (F/F) and heterozygously targeted ES cells (+/-).

Dilution buffer : PBS-Tween containing 5% skimmed milk.

All lanes:

Western blot - Anti-KMT2D / MLL2 antibody (ab231239) at 1/500 dilution

Lane 1:

Mouse embryonic stem cells (E14Tg2a) whole cell lysate (Heterozygous knockout)

Lanes 2 - 3:

Mouse embryonic stem cells (E14Tg2a) whole cell lysate (Homozygous knockout)

Lane 4:

Mouse embryonic stem cells (E14Tg2a) whole cell lysate (flp recombined)

Predicted band size: 593 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Synthetic Peptide within Mouse Kmt2d conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q6PDK2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Preservative: 0.05% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The KMT2D also known as MLL2 is an important histone methyltransferase with a known mass of approximately 553 kDa. This enzyme plays a mechanical role in the methylation of histone H3 on lysine 4 (H3K4) which facilitates chromatin remodeling and transcriptional regulation. KMT2D is expressed in various tissues including the liver lungs and heart indicative of its diverse regulatory roles across different biological systems.
Biological function summary

KMT2D is an important regulatory factor that influences gene expression. It is part of a larger complex that includes other interacting proteins necessary for histone modification. Through its enzymatic activity KMT2D impacts gene transcription by modifying chromatin structure making it more or less accessible for transcription factors to bind DNA. This control over gene expression has implications in development differentiation and cellular response to external stimuli.

Pathways

KMT2D integrates into the broader framework of chromatin modification and transcriptional regulation pathways. It interacts with proteins such as ASH2L and RBBP5 which are components of the COMPASS-like complex playing roles in modifying histones to regulate gene expression dynamically. KMT2D's function intersects particularly with the Hedgehog and Wnt signaling pathways both important for developmental processes and cellular proliferation.

KMT2D has strong associations with several conditions. Mutations or dysregulation of this methyltransferase can lead to developmental disorders such as Kabuki syndrome characterized by intellectual disability and growth delays. KMT2D anomalies also appear in cancer where its dysregulation affects pathways involving the enhancer of filament genes (EFG) and histone methyltransferases like NSD2 contributing to oncogenesis by altering gene expression profiles critical for cell growth.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Histone methyltransferase that catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4). Part of chromatin remodeling machinery predominantly forms H3K4me1 methylation marks at active chromatin sites where transcription and DNA repair take place. Acts as a coactivator for estrogen receptor by being recruited by ESR1, thereby activating transcription.
See full target information Kmt2d
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell transplantation 30:9636897211027521 PubMed34705580

2021

Histone Methyltransferase KMT2D Regulates H3K4 Methylation and is Involved in the Pathogenesis of Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Li,Mengdie Shi,Ying Xu,Jianping Qiu,Qing Lv
View all publications
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Product promise

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