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AB243044

Anti-KMT2D / MLL2 antibody [CL5346]

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Mouse Monoclonal KMT2D / MLL2 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human KMT2D aa 4350-4500.

View Alternative Names

ALR, MLL2, MLL4, KMT2D, Histone-lysine N-methyltransferase 2D, Lysine N-methyltransferase 2D, ALL1-related protein, Myeloid/lymphoid or mixed-lineage leukemia protein 2

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-KMT2D / MLL2 antibody [CL5346] (AB243044)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KMT2D / MLL2 antibody [CL5346] (AB243044)

Paraformaldehyde-fixed, Triton X-100 permeabilized Caco-2 ( Human colorectal adenocarcinoma cell line) cells labeling KMT2D / MLL2 (Green) using ab243044 at 10 μg/ml in ICC/IF analysis.

Microtubules are visualized in red.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL5346

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human KMT2D aa 4350-4500. The exact immunogen used to generate this antibody is proprietary information.

O14686

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified from TCS.
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The KMT2D also known as MLL2 is an important histone methyltransferase with a known mass of approximately 553 kDa. This enzyme plays a mechanical role in the methylation of histone H3 on lysine 4 (H3K4) which facilitates chromatin remodeling and transcriptional regulation. KMT2D is expressed in various tissues including the liver lungs and heart indicative of its diverse regulatory roles across different biological systems.
Biological function summary

KMT2D is an important regulatory factor that influences gene expression. It is part of a larger complex that includes other interacting proteins necessary for histone modification. Through its enzymatic activity KMT2D impacts gene transcription by modifying chromatin structure making it more or less accessible for transcription factors to bind DNA. This control over gene expression has implications in development differentiation and cellular response to external stimuli.

Pathways

KMT2D integrates into the broader framework of chromatin modification and transcriptional regulation pathways. It interacts with proteins such as ASH2L and RBBP5 which are components of the COMPASS-like complex playing roles in modifying histones to regulate gene expression dynamically. KMT2D's function intersects particularly with the Hedgehog and Wnt signaling pathways both important for developmental processes and cellular proliferation.

KMT2D has strong associations with several conditions. Mutations or dysregulation of this methyltransferase can lead to developmental disorders such as Kabuki syndrome characterized by intellectual disability and growth delays. KMT2D anomalies also appear in cancer where its dysregulation affects pathways involving the enhancer of filament genes (EFG) and histone methyltransferases like NSD2 contributing to oncogenesis by altering gene expression profiles critical for cell growth.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Histone methyltransferase that catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) (PubMed : 25561738). Part of chromatin remodeling machinery predominantly forms H3K4me1 methylation marks at active chromatin sites where transcription and DNA repair take place (PubMed : 17500065, PubMed : 25561738). Acts as a coactivator for estrogen receptor by being recruited by ESR1, thereby activating transcription (PubMed : 16603732).
See full target information KMT2D
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