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Anti-KMT2D / MLL2 antibody [EPR20604-73] (ab213721) is a rabbit monoclonal antibody that is used to detect KMT2D / MLL2 in Western Blot. Suitable for Human, samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

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Images

Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (AB213721), expandable thumbnail
  • Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (AB213721), expandable thumbnail
  • Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (AB213721), expandable thumbnail
  • Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (AB213721), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-PFlow CytIP
Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Recombinant fragment - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
1/1000
Notes

See western-blot-for-high-molecular-weights for very high molecular weight proteins (>300 kDa).

Species
Human
Dilution info
1/1000
Notes

See western-blot-for-high-molecular-weights for very high molecular weight proteins (>300 kDa).

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Recombinant fragment - Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Recombinant fragment - Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Recombinant fragment - Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Histone methyltransferase that catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) (PubMed:25561738). Part of chromatin remodeling machinery predominantly forms H3K4me1 methylation marks at active chromatin sites where transcription and DNA repair take place (PubMed:17500065, PubMed:25561738). Acts as a coactivator for estrogen receptor by being recruited by ESR1, thereby activating transcription (PubMed:16603732).

Alternative names

ALR, MLL2, MLL4, KMT2D, Histone-lysine N-methyltransferase 2D, Lysine N-methyltransferase 2D, ALL1-related protein, Myeloid/lymphoid or mixed-lineage leukemia protein 2

Recommended products

Anti-KMT2D / MLL2 antibody [EPR20604-73] (ab213721) is a rabbit monoclonal antibody that is used to detect KMT2D / MLL2 in Western Blot. Suitable for Human, samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR20604-73
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-KMT2D / MLL2 antibody [EPR20604-73] (ab213721) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), in Human, samples.

What is the molecular weight of KMT2D / MLL2?


Anti-KMT2D / MLL2 [EPR20604-73] (ab213721) specifically detects a band for KMT2D / MLL2 (UniProt: O14686) at a molecular weight of 593kDa.

Recommended positive controls


WB: HAP1 and HEK-293A whole cell lysates, 293T (Human embryonic kidney epithelial cell) transfected with an empty vector, containing a myc-His-tag®, whole cell lysate, GST-tagged recombinant human KMT2D (MLL2) protein (aa 5355 to 5537).

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products


We have a range of other formats of antibody clone [EPR20604-73] also available for your convenience:
ab213721, Carrier free - Anti-KMT2D / MLL2 antibody [EPR20604-73] - BSA and Azide free ab274431



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The KMT2D also known as MLL2 is an important histone methyltransferase with a known mass of approximately 553 kDa. This enzyme plays a mechanical role in the methylation of histone H3 on lysine 4 (H3K4) which facilitates chromatin remodeling and transcriptional regulation. KMT2D is expressed in various tissues including the liver lungs and heart indicative of its diverse regulatory roles across different biological systems.

Biological function summary

KMT2D is an important regulatory factor that influences gene expression. It is part of a larger complex that includes other interacting proteins necessary for histone modification. Through its enzymatic activity KMT2D impacts gene transcription by modifying chromatin structure making it more or less accessible for transcription factors to bind DNA. This control over gene expression has implications in development differentiation and cellular response to external stimuli.

Pathways

KMT2D integrates into the broader framework of chromatin modification and transcriptional regulation pathways. It interacts with proteins such as ASH2L and RBBP5 which are components of the COMPASS-like complex playing roles in modifying histones to regulate gene expression dynamically. KMT2D's function intersects particularly with the Hedgehog and Wnt signaling pathways both important for developmental processes and cellular proliferation.

Associated diseases and disorders

KMT2D has strong associations with several conditions. Mutations or dysregulation of this methyltransferase can lead to developmental disorders such as Kabuki syndrome characterized by intellectual disability and growth delays. KMT2D anomalies also appear in cancer where its dysregulation affects pathways involving the enhancer of filament genes (EFG) and histone methyltransferases like NSD2 contributing to oncogenesis by altering gene expression profiles critical for cell growth.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (ab213721), expandable thumbnail

    Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (ab213721)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (ab213721) at 1/50000 dilution

    Lane 1: 293T (Human embryonic kidney epithelial cell) transfected with an empty vector, containing a myc-His-tag®, whole cell lysate at 15 µg

    Lane 2: 293T transfected with KMT2D (MLL2) expression vector (aa 5175 to 5537) containing a myc-His-tag®, whole cell lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 593 kDa

    Exposure time: 10s

  • Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (ab213721), expandable thumbnail

    Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (ab213721)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (ab213721) at 1/50000 dilution

    All lanes: GST-tagged recombinant human KMT2D (MLL2) protein (aa 5355 to 5537) 10ng at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 593 kDa

    Exposure time: 10s

  • Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (ab213721), expandable thumbnail

    Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (ab213721)

    The protein is loaded in Tris-Acetate gel, run at 150 V for 3 hours, then transferred on PVDF membrane at 40 V overnight. After blocking in 10% milk/TBST for 1 hour, the membrane is incubated with primary antibody overnight at 4°C.

    Exposure time: 15 seconds

    All lanes: Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (ab213721) at 1/1000 dilution

    All lanes: HEK-293A (human embryonic kidney epithelial cell, subclone), whole cell lysate at 60 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 593 kDa

    Observed band size: 600 kDa

  • Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (ab213721), expandable thumbnail

    Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (ab213721)

    The protein is loaded in Tris-Acetate gel, run at 150 V for 3 hours, then transferred on PVDF membrane at 40 V overnight. After blocking in 10% milk/TBST for 1 hour, the membrane is incubated with primary antibody overnight at 4°C.

    Exposure time: 15 seconds

    All lanes: Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (ab213721) at 1/1000 dilution

    All lanes: HAP1 (human chronic myelogenous leukemia near-haploid cell line) whole cell lysate at 60 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 593 kDa

    Observed band size: 600 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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