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AB213721

Anti-KMT2D / MLL2 antibody [EPR20604-73]

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(5 Publications)

Anti-KMT2D / MLL2 antibody [EPR20604-73] (ab213721) is a rabbit monoclonal antibody detecting KMT2D / MLL2 in Western Blot. Suitable for Human,.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

ALR, MLL2, MLL4, KMT2D, Histone-lysine N-methyltransferase 2D, Lysine N-methyltransferase 2D, ALL1-related protein, Myeloid/lymphoid or mixed-lineage leukemia protein 2

4 Images
Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (AB213721)
  • WB

Lab

Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (AB213721)

The protein is loaded in Tris-Acetate gel, run at 150 V for 3 hours, then transferred on PVDF membrane at 40 V overnight. After blocking in 10% milk/TBST for 1 hour, the membrane is incubated with primary antibody overnight at 4°C.

Exposure time : 15 seconds

All lanes:

Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (ab213721) at 1/1000 dilution

All lanes:

HAP1 (human chronic myelogenous leukemia near-haploid cell line) whole cell lysate at 60 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 593 kDa

Observed band size: 600 kDa

false

Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (AB213721)
  • WB

Lab

Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (AB213721)

The protein is loaded in Tris-Acetate gel, run at 150 V for 3 hours, then transferred on PVDF membrane at 40 V overnight. After blocking in 10% milk/TBST for 1 hour, the membrane is incubated with primary antibody overnight at 4°C.

Exposure time : 15 seconds

All lanes:

Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (ab213721) at 1/1000 dilution

All lanes:

HEK-293A (human embryonic kidney epithelial cell, subclone), whole cell lysate at 60 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 593 kDa

Observed band size: 600 kDa

false

Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (AB213721)
  • WB

Lab

Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (AB213721)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (ab213721) at 1/50000 dilution

All lanes:

GST-tagged recombinant human KMT2D (MLL2) protein (aa 5355 to 5537) 10ng at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 593 kDa

false

Exposure time: 10s

Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (AB213721)
  • WB

Lab

Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (AB213721)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (ab213721) at 1/50000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) transfected with an empty vector, containing a myc-His-tag®, whole cell lysate at 15 µg

Lane 2:

293T transfected with KMT2D (MLL2) expression vector (aa 5175 to 5537) containing a myc-His-tag®, whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 593 kDa

false

Exposure time: 10s

  • Carrier free

    Anti-KMT2D / MLL2 antibody [EPR20604-73] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20604-73

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-KMT2D / MLL2 antibody [EPR20604-73] (ab213721) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human, samples.

What is the molecular weight of KMT2D / MLL2?
Anti-KMT2D / MLL2 [EPR20604-73] (ab213721) specifically detects a band for KMT2D / MLL2 (UniProt: O14686) at a molecular weight of 593kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR20604-73] also available for your convenience: ab213721, Carrier free - ab274431

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The KMT2D also known as MLL2 is an important histone methyltransferase with a known mass of approximately 553 kDa. This enzyme plays a mechanical role in the methylation of histone H3 on lysine 4 (H3K4) which facilitates chromatin remodeling and transcriptional regulation. KMT2D is expressed in various tissues including the liver lungs and heart indicative of its diverse regulatory roles across different biological systems.
Biological function summary

KMT2D is an important regulatory factor that influences gene expression. It is part of a larger complex that includes other interacting proteins necessary for histone modification. Through its enzymatic activity KMT2D impacts gene transcription by modifying chromatin structure making it more or less accessible for transcription factors to bind DNA. This control over gene expression has implications in development differentiation and cellular response to external stimuli.

Pathways

KMT2D integrates into the broader framework of chromatin modification and transcriptional regulation pathways. It interacts with proteins such as ASH2L and RBBP5 which are components of the COMPASS-like complex playing roles in modifying histones to regulate gene expression dynamically. KMT2D's function intersects particularly with the Hedgehog and Wnt signaling pathways both important for developmental processes and cellular proliferation.

KMT2D has strong associations with several conditions. Mutations or dysregulation of this methyltransferase can lead to developmental disorders such as Kabuki syndrome characterized by intellectual disability and growth delays. KMT2D anomalies also appear in cancer where its dysregulation affects pathways involving the enhancer of filament genes (EFG) and histone methyltransferases like NSD2 contributing to oncogenesis by altering gene expression profiles critical for cell growth.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Histone methyltransferase that catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) (PubMed : 25561738). Part of chromatin remodeling machinery predominantly forms H3K4me1 methylation marks at active chromatin sites where transcription and DNA repair take place (PubMed : 17500065, PubMed : 25561738). Acts as a coactivator for estrogen receptor by being recruited by ESR1, thereby activating transcription (PubMed : 16603732).
See full target information KMT2D
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 21:e1011608 PubMed40971994

2025

Epigenome and transcriptome changes in KMT2D-related Kabuki syndrome Type 1 iPSCs, neuronal progenitors and cortical neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Cuvertino,Evgenii Martirosian,Kedar Bhosale,Peiwen Cheng,Terence Garner,Ian J Donaldson,Adam Jackson,Adam Stevens,Andrew D Sharrocks,Susan J Kimber,Siddharth Banka

Iranian journal of pharmaceutical research : IJPR 24:e159395 PubMed40718439

2025

KMT2D Induces M1 Macrophage Polarization to Repress Non-small Cell Lung Cancer Progression via Transcription Activation of ITGAL.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Tao Wang,Jie Yang,Peng-Fei Jiang

Frontiers in genetics 15:1522809 PubMed39867575

2025

Comprehensive analysis of ferroptosis-related genes reveals potential therapeutic targets in osteoporosis patients: a computational analysis and experiments.

Applications

Unspecified application

Species

Unspecified reactive species

Sihui Chen,Yi Jiang,Guoqin Xie,Peng Wu,Jinyu Zhu

iScience 27:110276 PubMed39109172

2024

Loss of ZNF408 attenuates STING-mediated immune surveillance in breast carcinogenesis.

Applications

WB

Species

Human

Xiao Cheng,Chunyu Yu,Yan Zhang,Yani Peng,Yuncheng Liu,Hangwei Fa,Lu Xia,Leyi Qin,Sudun Guan,Xiaodi Wu,Jiajing Wu,Yue Wang,Jianying Liu,Luyang Sun,Jing Liang,Yongfeng Shang

Cancer biology & therapy 24:2216041 PubMed37287122

2023

CSTF2T facilitates pancreatic adenocarcinoma growth and metastasis by elevating H3K4Me1 methylation of CALB2 via ASH2L.

Applications

WB

Species

Human

Wei Liu,Dongwei Hu,Jinjie Li,Qigang Xu,Zongjing Chen,Bo Xu
View all publications
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