Anti-KMT2D / MLL2 antibody [EPR20604-73] - BSA and Azide free

• WB

Lab

Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] - BSA and Azide free (AB274431)

This data was developed using ab213721, the same antibody clone in a different buffer formulation.

The protein is loaded in Tris-Acetate gel, run at 150 V for 3 hours, then transferred on PVDF membrane at 40 V overnight. After blocking in 10% milk/TBST for 1 hour, the membrane is incubated with primary antibody overnight at 4°C.

Exposure time : 15 seconds

All lanes:

Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (<a href='/en-us/products/primary-antibodies/kmt2d-mll2-antibody-epr20604-73-ab213721'>ab213721</a>) at 1/1000 dilution

All lanes:

HAP1 (human chronic myelogenous leukemia near-haploid cell line) whole cell lysate at 60 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 593 kDa

Observed band size: 600 kDa

false

• WB

Lab

Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] - BSA and Azide free (AB274431)

This data was developed using ab213721, the same antibody clone in a different buffer formulation.

The protein is loaded in Tris-Acetate gel, run at 150 V for 3 hours, then transferred on PVDF membrane at 40 V overnight. After blocking in 10% milk/TBST for 1 hour, the membrane is incubated with primary antibody overnight at 4°C.

Exposure time : 15 seconds

All lanes:

Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] (<a href='/en-us/products/primary-antibodies/kmt2d-mll2-antibody-epr20604-73-ab213721'>ab213721</a>) at 1/1000 dilution

All lanes:

HEK-293A (human embryonic kidney epithelial cell, subclone), whole cell lysate at 60 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 593 kDa

Observed band size: 600 kDa

false

• ChIC/CUT&RUN-seq

Supplier Data

ChIC/CUT&RUN sequencing - Anti-KMT2D / MLL2 antibody [EPR20604-73] - BSA and Azide free (AB274431)

This data was developed using ab213721, the same antibody clone in a different buffer formulation.

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10⁵ HeLa (human cervical adenocarcinoma epithelial cell) cells and 5 µg of ab213721 [EPR20604-63]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The binding loci are refered to PMID : 36598580 and PMID : 39117659.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

• ChIC/CUT&RUN-seq

Supplier Data

ChIC/CUT&RUN sequencing - Anti-KMT2D / MLL2 antibody [EPR20604-73] - BSA and Azide free (AB274431)

This data was developed using ab213721, the same antibody clone in a different buffer formulation.

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10⁵ HeLa (human cervical adenocarcinoma epithelial cell) cells and 5 µg of ab213721 [EPR20604-63]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The binding loci are refered to PMID : 36598580 and PMID : 39117659.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

• ChIC/CUT&RUN-seq

Supplier Data

ChIC/CUT&RUN sequencing - Anti-KMT2D / MLL2 antibody [EPR20604-73] - BSA and Azide free (AB274431)

This data was developed using ab213721, the same antibody clone in a different buffer formulation.

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10⁵ HeLa (human cervical adenocarcinoma epithelial cell) cells and 5 µg of ab213721 [EPR20604-63]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The binding loci are refered to PMID : 36598580 and PMID : 39117659.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.