Anti-KMT2D / MLL2 antibody [RP23040123]

3 product images

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  Immunocytochemistry/ Immunofluorescence - Anti-KMT2D / MLL2 antibody [RP23040123] (ab313444)

  Immunofluorescent analysis of 70% confluent log phase MCF-7 cells fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature labeling Histone-lysine N-methyltransferase 2D with ab313444 at 1/100 dilution followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 at 1/2000 dilution for 45 minutes at room temperature (Panel a/ Green). Nuclei (Panel b/Blue) were stained with DAPI. F-actin (Panel c/ Red) was stained with Rhodamine Phalloidin at 1/300 dilution. Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

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  Immunocytochemistry/ Immunofluorescence - Anti-KMT2D / MLL2 antibody [RP23040123] (ab313444)

  Immunofluorescent analysis of 70% confluent log phase A-431 cells fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature labeling Histone-lysine N-methyltransferase 2D with ab313444 at 1/100 dilution followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 at 1/2000 dilution for 45 minutes at room temperature (Panel a/ Green). Nuclei (Panel b/Blue) were stained with DAPI. F-actin (Panel c/ Red) was stained with Rhodamine Phalloidin at 1/300 dilution. Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

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  Immunocytochemistry/ Immunofluorescence - Anti-KMT2D / MLL2 antibody [RP23040123] (ab313444)

  Immunofluorescent analysis of HepG2 cells fixed using 4% formaldehyde (reconstituted in 1X PBS) for 10 min at room temperature and permeabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling KMT2D with ab313444 at 2 ?g/mL followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at 1/2000 dilution. Panel a) shows representative cells that were stained for detection and localization of KMT2D protein (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor 555 Rhodamine Phalloidin at 1/300 dilution. Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of KMT2D. Panel e) represents control cells with no primary antibody to assess background.