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Rabbit Recombinant Monoclonal KMT3C / SMYD2 antibody. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

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Images

Western blot - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (AB259973), expandable thumbnail
  • Western blot - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (AB259973), expandable thumbnail
  • Western blot - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (AB259973), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (AB259973), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (AB259973), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Not recommended
Tested
Mouse
Tested
Tested
Tested
Not recommended
Tested
Rat
Tested
Expected
Tested
Not recommended
Tested

Tested
Tested

Species

Mouse

Dilution info

1/50

Notes

-

Species

Rat

Dilution info

1/50

Notes

-

Species

Human

Dilution info

1/50

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/30

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

-

Species

Rat

Dilution info

1/500

Notes

-

Species

Human

Dilution info

1/500

Notes

-

Target data

Function

Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. Specifically trimethylates histone H3 'Lys-4' (H3K4me3) in vivo. The activity requires interaction with HSP90alpha. Shows even higher methyltransferase activity on p53/TP53. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates RB1 at 'Lys-860'.

Alternative names

N-lysine methyltransferase SMYD2, HSKM-B, Histone methyltransferase SMYD2, Lysine N-methyltransferase 3C, SET and MYND domain-containing protein 2, KMT3C, SMYD2

Recommended products

Rabbit Recombinant Monoclonal KMT3C / SMYD2 antibody. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR24071-25

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

KMT3C also known as SMYD2 is a lysine methyltransferase enzyme that possesses a unique SET domain responsible for catalyzing the methylation of lysine residues on histone and non-histone proteins. The enzyme has a molecular mass of approximately 52 kDa. SMYD2 shows expression in various tissues including heart skeletal muscle and also liver. It methylates histone H3 on lysine 36 (H3K36) and several non-histone proteins influencing gene expression regulation and protein function.

Biological function summary

KMT3C/SMYD2 plays important roles in gene regulation and cellular processes. It functions as a part of a protein complex affecting transcriptional activation and repression via chromatin remodeling. The methylation activity of SMYD2 extends to p53 an important tumor suppressor linking it to the regulation of cell cycle and apoptosis. This enzymatic activity impacts cellular growth and differentiation interfacing with the larger network of cellular gene expression control and protein function modulation.

Pathways

Research shows KMT3C/SMYD2 being active in the p53 and AKT signaling pathways fundamental to cell survival proliferation and apoptosis. In the context of the p53 pathway SMYD2 methylates p53 which may impact its tumor suppressor functions. SMYD2 also interacts with proteins like RB1 and HDAC1 integrating into broader cell regulatory networks. These interactions facilitate SMYD2's influence on pathways that govern important cellular processes such as growth and DNA damage response.

Associated diseases and disorders

Mutations and overexpression of KMT3C/SMYD2 associate with various cancers including breast and esophageal cancer. In the case of breast cancer SMYD2 modulates p53 and RB1 activity contributing to tumorigenesis. Furthermore its interaction with HDAC1 connects it with epigenetic dysregulation seen in cancer progression. Altered activity or expression of SMYD2 disrupts normal cellular function providing a link between its biological activity and oncogenic processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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10 product images

  • Western blot - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (ab259973), expandable thumbnail

    Western blot - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (ab259973)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 29487338)

    Exposure time: Lanes 1-4: 37 seconds; Lanes 5-9: 15 seconds.

    All lanes: Western blot - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (ab259973) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 5: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

    Lane 6: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Lane 7: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 8: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

    Lane 9: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 49 kDa

    This data was developed using 259973, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 29487338)

    Exposure time: Lanes 1-4: 37 seconds; Lanes 5-9: 15 seconds.

  • Western blot - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (ab259973), expandable thumbnail

    Western blot - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (ab259973)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 28588028)

    Exposure time: 1 second

    All lanes: Western blot - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (ab259973) at 1/1000 dilution

    Lane 1: Human brain tissue lysate at 20 µg

    Lane 2: Human heart tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 49 kDa

  • Western blot - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (ab259973), expandable thumbnail

    Western blot - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (ab259973)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22241783)

    Exposure time: 8 seconds

    All lanes: Western blot - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (ab259973) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Mouse heart tissue lysate at 20 µg

    Lane 3: Mouse spleen tissue lysate at 20 µg

    Lane 4: Rat brain tissue lysate at 20 µg

    Lane 5: Rat heart tissue lysate at 20 µg

    Lane 6: Rat kidney tissue lysate at 20 µg

    Lane 7: Rat spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 49 kDa

    This data was developed using 259973, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22241783)

    Exposure time: 8 seconds

  • Immunocytochemistry/ Immunofluorescence - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (ab259973), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (ab259973)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 cells labelling KMT3C/SMYD2 with ab259973 at 1/50 (11.82 μg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing mainly cytoplasmic staining in HepG2 cells is observed.

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (ab259973), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (ab259973)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling KMT3C/SMYD2 with ab259973 at 1/50 (11.82 μg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing mainly cytoplasmic staining in NIH/3T3 cells is observed.

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (ab259973), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (ab259973)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C6 cells labelling KMT3C/SMYD2 with ab259973 at 1/50 (11.82 μg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing mainly cytoplasmic staining in C6 cells is observed.

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (ab259973), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (ab259973)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling KMT3C/SMYD2 with ab259973 at 1/500 dilution (0.1μg)/ (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (ab259973), expandable thumbnail

    Immunoprecipitation - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (ab259973)

    KMT3C/SMYD2 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug with ab259973 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259973 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug

    Lane 2: ab259973 IP in NIH/3T3 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab259973 in NIH/3T3 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds

    All lanes: Immunoprecipitation - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (ab259973)

    Predicted band size: 49 kDa

  • Flow Cytometry (Intracellular) - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (ab259973), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (ab259973)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling KMT3C/SMYD2 with ab259973 at 1/500 dilution (0.1μg)/ (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (ab259973), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-KMT3C / SMYD2 antibody [EPR24071-25] (ab259973)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C6 (Rat glial tumor glial cell) cells labelling KMT3C/SMYD2 with ab259973 at 1/500 dilution (0.1μg)/ (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

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