Rabbit Polyclonal KMT4 / Dot1L antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human DOT1L aa 1000-1050.
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- Cancer cell international 22:3362022Combinatorial targeting of menin and the histone methyltransferase DOT1L as a novel therapeutic strategy for treatment of chemotherapy-resistant ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElena Alexandrova,Jessica Lamberti,Domenico Memoli,Claudia Quercia,Viola Melone,Francesca Rizzo,Roberta Tarallo,Giorgio Giurato,Giovanni Nassa,Alessandro WeiszPubMed 36333801
- Breast cancer research : BCR 24:522022Combinatorial targeting of a chromatin complex comprising Dot1L, menin and the tyrosine kinase BAZ1B reveals a new therapeutic vulnerability of endocrine therapy-resistant breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnnamaria Salvati,Viola Melone,Assunta Sellitto,Francesca Rizzo,Roberta Tarallo,Tuula A Nyman,Giorgio Giurato,Giovanni Nassa,Alessandro WeiszPubMed 35850772
- RSC chemical biology 3:456-4672022A novel screening strategy to identify histone methyltransferase inhibitors reveals a crosstalk between DOT1L and CARM1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Si,Corentin Bon,Magdalena Barbachowska,Veronique Cadet-Daniel,Corinne Jallet,Laura Soresinetti,Mikaël Boullé,Magalie Duchateau,Mariette Matondo,Fabrice Agou,Ludovic Halby,Paola B ArimondoPubMed 35441144
- The Journal of biological chemistry 295:17973-179852020Inhibition of the SUV4-20 H1 histone methyltransferase increases frataxin expression in Friedreich's ataxia patient cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGabriela Vilema-Enríquez et. al.PubMed 33028632
- Nature communications 11:41532020Histone methyltransferase DOT1L coordinates AR and MYC stability in prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesR Vatapalli et. al.PubMed 32814769
- Cancers 11:2019The Histone Methyltransferase DOT1L Is a Functional Component of Estrogen Receptor Alpha Signaling in Ovarian Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnnamaria Salvati et. al.PubMed 31689915
- Science advances 5:eaav55902019Inhibition of histone methyltransferase DOT1L silences ERα gene and blocks proliferation of antiestrogen-resistant breast cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiovanni Nassa et. al.PubMed 30775443
- Nature communications 9:44292018Nuclear lactate dehydrogenase A senses ROS to produce α-hydroxybutyrate for HPV-induced cervical tumor growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan Liu et. al.PubMed 30356100
- Nature communications 9:17392018C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDan Liu et. al.PubMed 29712898
- Nature communications 9:2592018The protective role of DOT1L in UV-induced melanomagenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Zhu et. al.PubMed 29343685
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human DOT1L aa 1000-1050. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TEK3
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Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2-5 µg/mg of lysate | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 - 1/10000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 - 1/1000 | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones (PubMed:12123582). Binds to DNA (PubMed:12628190).
Alternative names
KIAA1814, KMT4, DOT1L, DOT1-like protein, Histone H3-K79 methyltransferase, Lysine N-methyltransferase 4, H3-K79-HMTase
Recommended products
Rabbit Polyclonal KMT4 / Dot1L antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human DOT1L aa 1000-1050.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human DOT1L aa 1000-1050. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TEK3
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KMT4 also known as Dot1L is a histone methyltransferase that catalyzes the methylation of histone H3 at lysine 79. This protein plays a significant role in chromatin remodeling and gene expression regulation. Dot1L has a molecular mass of approximately 171 kDa. It expresses widely in various tissues with high presence in the heart brain and testes indicating its importance in diverse cellular processes.
- Biological function summary
Dot1L functions as a critical enzyme influencing transcriptional activation and repression. It does not require a specific formed complex to execute its activity unlike many other methyltransferases that work as part of larger protein complexes. Dot1L interacts directly with nucleosomes and influences gene expression by modifying chromatin structure. This regulation of gene expression is important for the coordination of normal developmental processes and cellular proliferation.
- Pathways
Dot1L impacts epigenetic regulation and is integral to the Wnt signaling pathway. This pathway is essential for processes like embryonic development and cell differentiation. Dot1L also plays an essential role in DNA damage response and repair pathways. It collaborates with other proteins like BRG1 a chromatin re-modeling factor to initiate specific genomic responses related to transcriptional control genome stability and repair mechanisms.
- Associated diseases and disorders
Dot1L has been linked to mixed lineage leukemia (MLL) and other types of hematological malignancies. The aberrant activity of Dot1L can disrupt normal gene regulatory mechanisms leading to uncontrolled cell proliferation and cancer. Dot1L interacts with MLL fusion proteins that result from chromosomal translocations which are often present in leukemia. Abnormal Dot1L activity is also associated with the progression of other cancers making it a potential therapeutic target in oncology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT4 / Dot1L antibody (ab72454)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling KMT4 / Dot1L with ab72454 at 1/200 (1µg/ml). Detection: DAB.
Immunoprecipitation - Anti-KMT4 / Dot1L antibody (ab72454)
HeLa whole cell lysate ( 1 mg for IP, 20% of IP loaded) with ab72454 at 3 µg/mg lysate for IP, followed by ab72454 at 1 µg/ml for WB.
Detection: Chemiluminescence with an exposure time of 10 seconds.All lanes: Immunoprecipitation - Anti-KMT4 / Dot1L antibody (ab72454)
Predicted band size: 185 kDa
Western blot - Anti-KMT4 / Dot1L antibody (ab72454)
All lanes: Western blot - Anti-KMT4 / Dot1L antibody (ab72454) at 0.04 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 185 kDa
Observed band size: 100 kDa, 185 kDa
Exposure time: 3min
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-KMT4 / Dot1L antibody (ab72454)
KMT4 / Dot1L western blot using anti-KMT4 / Dot1L antibody ab72454. Publication image and figure legend from Liu, D., Zhang, X. X., et al., 2018, Nat Commun, PubMed 29712898.
ab72454 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab72454 please see the product overview.
C/EBPβ recruits the methyltransferase DOT1L to target genes that methylate H3K79. a Venn diagrams showing the overlap of C/EBPβ- and DOT1L-targeted genes (chi-squared test). b Correlation analysis of C/EBPβ binding sites and DOT1L binding sites. Red dots represent genes with two peak centers located less than 2500 bp apart. c Representative results of C/EBPβ and DOT1L ChIP-qPCR. Primers were chosen according to the ChIP-seq results (upper panel). d C/EBPβ interacts with DOT1L. Lysates from C13* cells were immunoprecipitated (IP) with a mouse anti-C/EBPβ antibody and analyzed by western blot using a rabbit anti-DOT1L antibody (upper panel) or the reciprocal (lower panel). e ChIP-reChIP experiments with anti-C/EBPβ and anti-DOT1L antibodies. Mouse IgG (mIgG) and rabbit IgG (rIgG) were used as negative controls. f Meta-analysis of the averaged DOT1L ChIP-seq signal of genes across a ±10 kb genomic region flanking the TSS. g Normalized C/EBPβ and DOT1L ChIP-seq signal of the representative C/EBPβ-DOT1L co-targeted genes (EREG and SLC38A1). h Analysis of DOT1L ChIP-qPCR (green), H3K79me2/me3 ChIP-qPCR (blue), and RT-qPCR (purple) in C13* cells. Gene names in red indicate documented cisplatin-resistance genes in ovarian cancer. i The protein levels of C/EBPβ-DOT1L co-targeted genes in the indicated OV2008 cells were detected by western blot. Uncropped images of blots are shown in Supplementary Figure 25. *P < 0.05; ***P < 0.001
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