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Rabbit Recombinant Monoclonal KMT4 / Dot1L antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT4 / Dot1L antibody [EPR22936-138] (AB239358), expandable thumbnail
  • Western blot - Anti-KMT4 / Dot1L antibody [EPR22936-138] (AB239358), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT4 / Dot1L antibody [EPR22936-138] (AB239358), expandable thumbnail
  • Western blot - Anti-KMT4 / Dot1L antibody [EPR22936-138] (AB239358), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT4 / Dot1L antibody [EPR22936-138] (AB239358), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPChIPFlow CytWBICC/IF
Human
Tested
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Mouse
Tested
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Rat
Tested
Not recommended
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/200
Notes

In-house IHC data gave positive staining only on testis.

1/4000 for mouse and rat; 1/200 for human.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/200
Notes

In-house IHC data gave positive staining only on testis.

1/4000 for mouse and rat; 1/200 for human.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/200
Notes

In-house IHC data gave positive staining only on testis.

1/4000 for mouse and rat; 1/200 for human.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones (PubMed:12123582). Binds to DNA (PubMed:12628190).

Alternative names

KIAA1814, KMT4, DOT1L, DOT1-like protein, Histone H3-K79 methyltransferase, Lysine N-methyltransferase 4, H3-K79-HMTase

Recommended products

Rabbit Recombinant Monoclonal KMT4 / Dot1L antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22936-138
Purification technique
Affinity purification Protein A
Specificity

In-house IHC data gave positive staining only on testis.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

KMT4 also known as Dot1L is a histone methyltransferase that catalyzes the methylation of histone H3 at lysine 79. This protein plays a significant role in chromatin remodeling and gene expression regulation. Dot1L has a molecular mass of approximately 171 kDa. It expresses widely in various tissues with high presence in the heart brain and testes indicating its importance in diverse cellular processes.

Biological function summary

Dot1L functions as a critical enzyme influencing transcriptional activation and repression. It does not require a specific formed complex to execute its activity unlike many other methyltransferases that work as part of larger protein complexes. Dot1L interacts directly with nucleosomes and influences gene expression by modifying chromatin structure. This regulation of gene expression is important for the coordination of normal developmental processes and cellular proliferation.

Pathways

Dot1L impacts epigenetic regulation and is integral to the Wnt signaling pathway. This pathway is essential for processes like embryonic development and cell differentiation. Dot1L also plays an essential role in DNA damage response and repair pathways. It collaborates with other proteins like BRG1 a chromatin re-modeling factor to initiate specific genomic responses related to transcriptional control genome stability and repair mechanisms.

Associated diseases and disorders

Dot1L has been linked to mixed lineage leukemia (MLL) and other types of hematological malignancies. The aberrant activity of Dot1L can disrupt normal gene regulatory mechanisms leading to uncontrolled cell proliferation and cancer. Dot1L interacts with MLL fusion proteins that result from chromosomal translocations which are often present in leukemia. Abnormal Dot1L activity is also associated with the progression of other cancers making it a potential therapeutic target in oncology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT4 / Dot1L antibody [EPR22936-138] (ab239358), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT4 / Dot1L antibody [EPR22936-138] (ab239358)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling KMT4 / Dot1L with ab239358 at 1/250 dilution (2.78 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on spermatocytes of human testis. The section was incubated with ab239358 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins

  • Western blot - Anti-KMT4 / Dot1L antibody [EPR22936-138] (ab239358), expandable thumbnail

    Western blot - Anti-KMT4 / Dot1L antibody [EPR22936-138] (ab239358)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 26199140).

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-KMT4 / Dot1L antibody [EPR22936-138] (ab239358) at 1/1000 dilution

    All lanes: C6 (rat glial tumor glial cell), whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 185 kDa

    Observed band size: 185 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT4 / Dot1L antibody [EPR22936-138] (ab239358), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT4 / Dot1L antibody [EPR22936-138] (ab239358)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling KMT4 / Dot1L with ab239358 at 1/4000 dilution (0.17 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on spermatocytes of mouse testis. The section was incubated with ab239358 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins

  • Western blot - Anti-KMT4 / Dot1L antibody [EPR22936-138] (ab239358), expandable thumbnail

    Western blot - Anti-KMT4 / Dot1L antibody [EPR22936-138] (ab239358)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 26199140).

    Fresh lysates are recommended on WB test.

    Exposure time: 26 seconds.

    All lanes: Western blot - Anti-KMT4 / Dot1L antibody [EPR22936-138] (ab239358) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 2: 293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

    Lane 3: K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg

    Lane 4: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

    Lane 5: HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 6: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 185 kDa

    Observed band size: 185 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT4 / Dot1L antibody [EPR22936-138] (ab239358), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT4 / Dot1L antibody [EPR22936-138] (ab239358)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling KMT4 / Dot1L with ab239358 at 1/4000 dilution (0.17 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on spermatocytes of rat testis. The section was incubated with ab239358 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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