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Rabbit Recombinant Monoclonal KMT4 / Dot1L antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.

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Images

Sandwich ELISA - Anti-KMT4/Dot1L antibody [EPR22936-138] - BSA and Azide free (Detector) (AB305337), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Expected
Mouse
Expected
Rat
Expected

Expected
Expected

Species

Human, Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones (PubMed:12123582). Binds to DNA (PubMed:12628190).

Alternative names

DOT1-like protein, Histone H3-K79 methyltransferase, Lysine N-methyltransferase 4, H3-K79-HMTase, KMT4, KIAA1814, DOT1L

Recommended products

Rabbit Recombinant Monoclonal KMT4 / Dot1L antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.

Alternative names

DOT1-like protein, Histone H3-K79 methyltransferase, Lysine N-methyltransferase 4, H3-K79-HMTase, KMT4, KIAA1814, DOT1L

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR22936-138

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

KMT4 also known as Dot1L is a histone methyltransferase that catalyzes the methylation of histone H3 at lysine 79. This protein plays a significant role in chromatin remodeling and gene expression regulation. Dot1L has a molecular mass of approximately 171 kDa. It expresses widely in various tissues with high presence in the heart brain and testes indicating its importance in diverse cellular processes.

Biological function summary

Dot1L functions as a critical enzyme influencing transcriptional activation and repression. It does not require a specific formed complex to execute its activity unlike many other methyltransferases that work as part of larger protein complexes. Dot1L interacts directly with nucleosomes and influences gene expression by modifying chromatin structure. This regulation of gene expression is important for the coordination of normal developmental processes and cellular proliferation.

Pathways

Dot1L impacts epigenetic regulation and is integral to the Wnt signaling pathway. This pathway is essential for processes like embryonic development and cell differentiation. Dot1L also plays an essential role in DNA damage response and repair pathways. It collaborates with other proteins like BRG1 a chromatin re-modeling factor to initiate specific genomic responses related to transcriptional control genome stability and repair mechanisms.

Associated diseases and disorders

Dot1L has been linked to mixed lineage leukemia (MLL) and other types of hematological malignancies. The aberrant activity of Dot1L can disrupt normal gene regulatory mechanisms leading to uncontrolled cell proliferation and cancer. Dot1L interacts with MLL fusion proteins that result from chromosomal translocations which are often present in leukemia. Abnormal Dot1L activity is also associated with the progression of other cancers making it a potential therapeutic target in oncology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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