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AB111691

Anti-KMT5A / SETD8 / Pr-SET7 antibody

4

(3 Reviews)

|

(6 Publications)

Rabbit Polyclonal KMT5A / SETD8 / Pr-SET7 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KMT5A aa 50-300.

View Alternative Names

PRSET7, SET07, SET8, SETD8, KMT5A, N-lysine methyltransferase KMT5A, H4-K20-HMTase KMT5A, Histone-lysine N-methyltransferase KMT5A, Lysine N-methyltransferase 5A, Lysine-specific methylase 5A, PR/SET domain-containing protein 07, SET domain-containing protein 8, PR-Set7, PR/SET07

1 Images
Western blot - Anti-KMT5A / SETD8 / Pr-SET7 antibody (AB111691)
  • WB

Supplier Data

Western blot - Anti-KMT5A / SETD8 / Pr-SET7 antibody (AB111691)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-KMT5A / SETD8 / Pr-SET7 antibody (ab111691) at 1/2000 dilution

All lanes:

U87 MG Whole cell extract at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 39 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human KMT5A aa 50-300. The exact immunogen used to generate this antibody is proprietary information.

Q9NQR1

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KMT5A also known as SETD8 or Pr-SET7 is a histone methyltransferase enzyme with a notable role in regulating chromatin dynamics. It specifically catalyzes the monomethylation of histone H4 at lysine 20 (H4K20me1) a modification linked to chromatin compaction and DNA damage response. The molecular mass of KMT5A is approximately 47 kDa. Expression of KMT5A is observed in various tissues including the liver kidney and to some extent in embryonic stem cells indicating its functionality across different stages of development and cell types.
Biological function summary

KMT5A's activity influences several essential cellular processes such as cell cycle progression and DNA repair. As part of a larger chromatin-modulating framework it often functions alongside other chromatin-associated proteins including those from the Polycomb group. It plays a significant role in maintaining genomic stability by marking specific regions of chromatin for certain functions thereby affecting transcription regulation and cellular growth.

Pathways

KMT5A integrates into critical biological mechanisms like the DNA damage response and cell cycle regulation. It links closely with pathways involving p53 and cyclin-dependent kinase (CDK) inhibitors. KMT5A’s histone modification activity influences proteins such as p21 and p16 within the DNA damage signaling network. Its regulatory role here is essential as it facilitates an environment conducive to the DNA repair machinery following genotoxic stress.

KMT5A connects to various pathological states notably cancer and neurodegenerative disorders. Misregulation of KMT5A activity is associated with tumorigenesis where altered levels of H4K20me1 can lead to abnormal cell proliferation. Additionally KMT5A interacts through pathways tied to protein like TP53 influencing cellular responses to stressors typically seen in oncogenesis. Similarly aberrant regulation has implications in cognitive impairments potentially through dysregulated methylation patterns affecting neuronal cell cycle re-entry.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins (PubMed : 12086618, PubMed : 12121615, PubMed : 15964846, PubMed : 17707234, PubMed : 27338793). Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1) (PubMed : 12086618, PubMed : 12121615, PubMed : 15200950, PubMed : 15933069, PubMed : 15933070, PubMed : 15964846, PubMed : 16517599, PubMed : 27338793). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression (PubMed : 12086618, PubMed : 12121615, PubMed : 15200950, PubMed : 15933069, PubMed : 15933070, PubMed : 15964846, PubMed : 16517599). Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes (PubMed : 12086618, PubMed : 12121615, PubMed : 15200950, PubMed : 15933069, PubMed : 15933070, PubMed : 15964846, PubMed : 16517599). Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis (PubMed : 12086618, PubMed : 12121615, PubMed : 15200950, PubMed : 15933069, PubMed : 15933070, PubMed : 15964846, PubMed : 16517599). Involved in chromosome condensation and proper cytokinesis (PubMed : 12086618, PubMed : 12121615, PubMed : 15200950, PubMed : 15933069, PubMed : 15933070, PubMed : 15964846, PubMed : 16517599). Nucleosomes are preferred as substrate compared to free histones (PubMed : 12086618, PubMed : 12121615, PubMed : 15200950, PubMed : 15933069, PubMed : 15933070, PubMed : 15964846, PubMed : 16517599). Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes (PubMed : 17707234). Plays a negative role in TGF-beta response regulation and a positive role in cell migration (PubMed : 23478445).
See full target information KMT5A
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Neuroendocrinology 114:589-601 PubMed38565081

2024

Lysine Methyltransferase 5A Promotes the Progression of Growth Hormone Pituitary Neuroendocrine Tumors through the Wnt/β-Catenin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Junjun Li,Hao Song,Ting Chen,Sixi Zhang,Chao Zhang,Chen Ma,Lingye Zhang,Tengfei Wang,Yuan Qian,Xingli Deng

Molecular metabolism 80:101878 PubMed38218537

2024

Long non-coding RNA LncCplx2 regulates glucose homeostasis and pancreatic β cell function.

Applications

Unspecified application

Species

Unspecified reactive species

Linlin Wang,Liqiao Hu,Xingyue Wang,Zhaoxu Geng,Meng Wan,Junfeng Hao,Huisheng Liu,Yuying Fan,Tao Xu,Zonghong Li

Cell death & disease 12:580 PubMed34091587

2021

LncRNA LINC00473 is involved in the progression of invasive pituitary adenoma by upregulating KMT5A via ceRNA-mediated miR-502-3p evasion.

Applications

Unspecified application

Species

Unspecified reactive species

Junjun Li,Yuan Qian,Chao Zhang,Wei Wang,Yisheng Qiao,Hao Song,Liyan Li,Jiazhi Guo,Di Lu,Xingli Deng

Cell metabolism 33:160-173.e6 PubMed33406400

2021

Di-methylation of CD147-K234 Promotes the Progression of NSCLC by Enhancing Lactate Export.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Wang,Wan Huang,Ruo Chen,Peng Lin,Tao Zhang,Yun-Feng Ni,Hao Li,Jiao Wu,Xiu-Xuan Sun,Jie-Jie Geng,Yu-Meng Zhu,Gang Nan,Wei Zhang,Xi Chen,Ping Zhu,Huijie Bian,Zhi-Nan Chen

Journal of experimental & clinical cancer research 39:238 PubMed33168024

2020

MiRNA-340-5p mediates the functional and infiltrative promotion of tumor-infiltrating CD8 T lymphocytes in human diffuse large B cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yangyang Xu,Zhenchuan Liu,Lixin Lv,Ping Li,Bing Xiu,Wenbin Qian,Aibin Liang

American journal of human genetics 105:108-121 PubMed31204009

2019

RINT1 Bi-allelic Variations Cause Infantile-Onset Recurrent Acute Liver Failure and Skeletal Abnormalities.

Applications

Unspecified application

Species

Unspecified reactive species

Margot A Cousin,Erin Conboy,Jian-She Wang,Dominic Lenz,Tanya L Schwab,Monique Williams,Roshini S Abraham,Sarah Barnett,Mounif El-Youssef,Rondell P Graham,Luz Helena Gutierrez Sanchez,Linda Hasadsri,Georg F Hoffmann,Nathan C Hull,Robert Kopajtich,Reka Kovacs-Nagy,Jia-Qi Li,Daniela Marx-Berger,Valérie McLin,Mark A McNiven,Taofic Mounajjed,Holger Prokisch,Daisy Rymen,Ryan J Schulze,Christian Staufner,Ye Yang,Karl J Clark,Brendan C Lanpher,Eric W Klee
View all publications
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Product promise

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