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AB186006

Anti-KMT6 / EZH2 antibody

5

(5 Reviews)

|

(101 Publications)

Rabbit Polyclonal KMT6 / EZH2 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 101 publications. Immunogen corresponding to Synthetic Peptide within Human EZH2 aa 650 to C-terminus.

View Alternative Names

KMT6, EZH2, Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6 / EZH2 antibody (AB186006)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6 / EZH2 antibody (AB186006)

Human ovarian tissue stained with ab186006 at a diltution of 1 : 1000 to detect EZH2.

Immunoprecipitation - Anti-KMT6 / EZH2 antibody (AB186006)
  • IP

Supplier Data

Immunoprecipitation - Anti-KMT6 / EZH2 antibody (AB186006)

Detection of KMT6 / EZH2 by Western Blot of Immunprecipitate.

Lane 1 : ab186006 at 1 μg/ml labeling KMT6 / EZH2 in 293T whole cell lysate immunoprecipitated using ab186006 at 6μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

Lane 2 : Control IgG

Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-KMT6 / EZH2 antibody (ab186006)

Predicted band size: 85 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6 / EZH2 antibody (AB186006)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6 / EZH2 antibody (AB186006)

Mouse plasmacytoma tissue stained with ab186006 at a diltution of 1 : 1000 to detect EZH2.

Western blot - Anti-KMT6 / EZH2 antibody (AB186006)
  • WB

Supplier Data

Western blot - Anti-KMT6 / EZH2 antibody (AB186006)

Lane 1:

Western blot - Anti-KMT6 / EZH2 antibody (ab186006) at 1 µg

Lanes 2 - 4:

Western blot - Anti-KMT6 / EZH2 antibody (ab186006) at 1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

Mouse TCMK-1 cells.lysate at 50 µg

Predicted band size: 85 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, IP, WB

applications

Immunogen

Synthetic Peptide within Human EZH2 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15910

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KMT6 also known as EZH2 (Enhancer of Zeste Homolog 2) is a protein that functions as a histone methyltransferase. It catalyzes the methylation of histone H3 on lysine 27 (H3K27) a modification associated with gene silencing. EZH2 is a component of the Polycomb Repressive Complex 2 (PRC2) which plays an important role in chromatin remodeling and transcriptional repression. The EZH2 protein has a molecular weight of approximately 87 kDa and its gene is located on chromosome 7. In terms of expression EZH2 is found in various tissues but is notably abundant in growing and dividing cells.
Biological function summary

EZH2 plays a central role in regulating gene expression during development and differentiation. It is a core component of the PRC2 complex which includes other proteins such as EED and SUZ12. Through its catalytic activity EZH2 contributes to the maintenance of the transcriptionally repressed state of genes influencing processes such as cell identity and proliferation. In many cell types EZH2-mediated gene silencing is necessary for normal development and dysregulation can lead to aberrant cellular processes.

Pathways

EZH2 participates in key regulatory networks notably the Wnt signaling pathway and the p53 pathway. Within the Wnt pathway EZH2's activity contributes to controlling gene expression that influences cellular differentiation and proliferation. Meanwhile interaction with the p53 pathway can impact the cellular response to stress and DNA damage highlighting EZH2’s regulatory dimension. Other proteins like β-catenin in the Wnt pathway interact with EZH2 reflecting its integration in these complex signaling networks.

EZH2 has been linked to cancer and other proliferative diseases. Overexpression or mutation of EZH2 is frequently observed in cancers such as prostate and breast cancer where it contributes to unchecked cell growth and tumor progression. In these contexts proteins like p53 can interact abnormally with EZH2 leading to failure in tumor suppression mechanisms. Additionally deregulated EZH2 activity is implicated in certain developmental disorders highlighting its broader impact on cellular and organismal homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2 (PubMed : 22323599, PubMed : 30923826). Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.
See full target information EZH2
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Publications (101)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in endocrinology 16:1574938 PubMed40630095

2025

EZH2 promotes endometriosis progression through estrogen receptor and TNFα expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohan Liu,Liqin Cheng,Liuxuan Huang,Mingyue Li,Qingjun Shen,Donghan Li,Kailing Dai,Yanxia Fu,Min Li,Paul Yao,Liqin Zeng

International journal of chronic obstructive pulmonary disease 20:1543-1553 PubMed40417409

2025

A Peptide Encoded by lncRNA HOXB-AS3 Promotes Cigarette Smoke-Induced Inflammation in Bronchial Epithelial Cells via EZH2-Mediated H3K27me3 Modification.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Lin,Xiaoman Zhou,Yixiu Yang,Pingdong Xie,Quanni Li,Chanyi He,Qi Lin,Xingwei Wei,Yipeng Ding

Translational oncology 45:101962 PubMed38677015

2024

A novel LINC02321 promotes cell proliferation and decreases cisplatin sensitivity in bladder cancer by regulating RUVBL2.

Applications

Unspecified application

Species

Unspecified reactive species

Chuncheng Lu,Hongbin Gao,Haiyuan Li,Ning Luo,Shipeng Fan,Xi Li,Renbin Deng,Danpeng He,Hui Zhao

Journal of cellular and molecular medicine 28:e18216 PubMed38652219

2024

MELK aggravates lung adenocarcinoma by regulating EZH2 ubiquitination and H3K27me3 histone methylation of LATS2.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Yu,Xianrong Xu,Lirong Zhu,Shengjie Chen,Jincheng He

Clinical science (London, England : 1979) 138:251-268 PubMed38362910

2024

Inhibition of the histone methyltransferase EZH2 induces vascular stiffness.

Applications

Unspecified application

Species

Unspecified reactive species

Jaime Ibarrola,Rachel R Xiang,Zhe Sun,Qing Lu,Michael A Hill,Iris Z Jaffe

Journal of cellular and molecular medicine 27:3526-3538 PubMed37698037

2023

Inhibitory role of bone marrow mesenchymal stem cells-derived exosome in non-small-cell lung cancer: microRNA-30b-5p, EZH2 and PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tong Wu,Qi Tian,Ruiji Liu,Ke Xu,Shanshan Shi,Xiudi Zhang,Liming Gao,Xiaobo Yin,Shufeng Xu,Ping Wang

Cells 12: PubMed37759448

2023

Orai3 Calcium Channel Contributes to Oral/Oropharyngeal Cancer Stemness through the Elevation of ID1 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Anthony Nguyen,Youngjae Sung,Sung Hee Lee,Charlotte Ellen Martin,Sonal Srikanth,Wei Chen,Mo K Kang,Reuben H Kim,No-Hee Park,Yousang Gwack,Yong Kim,Ki-Hyuk Shin

Advanced healthcare materials 12:e2301137 PubMed37671812

2023

Fibrin Stiffness Regulates Phenotypic Plasticity of Metastatic Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Heilala,Arttu Lehtonen,Ossi Arasalo,Aino Peura,Juho Pokki,Olli Ikkala, Nonappa,Juha Klefström,Pauliina M Munne

Oncology letters 26:343 PubMed37427349

2023

CRISPR/Cas9‑mediated EZH2 knockout suppresses the proliferation and migration of triple‑negative breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qiqi Mao,Peibin Wu,Haochen Li,Xiaolan Fu,Xuechen Gao,Lei Yang

Nutrients 15: PubMed37375678

2023

Choline Regulates SOX4 through miR-129-5p and Modifies H3K27me3 in the Developing Cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Evan M Paules,Jorge A Silva-Gomez,Walter B Friday,Steve H Zeisel,Isis Trujillo-Gonzalez
View all publications
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