AB228697

Anti-KMT6 / EZH2 antibody

Name: Anti-KMT6 / EZH2 antibody (ab228697) | Abcam
Brand: Abcam Limited
SKU: AB228697
Price: 525 USD
Availability: InStock

(1 Review)

(16 Publications)

Rabbit Polyclonal KMT6 / EZH2 antibody. Suitable for ChIP, WB, ICC/IF and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EZH2.

View Alternative Names

KMT6, EZH2, Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-KMT6 / EZH2 antibody (AB228697)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KMT6 / EZH2 antibody (AB228697)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for KMT6 / EZH2 (green) using ab228697 at 1/200 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

Nuclear counterstain : Hoechst 33342 (blue).

ChIP - Anti-KMT6 / EZH2 antibody (AB228697)

ChIP

Supplier Data

ChIP - Anti-KMT6 / EZH2 antibody (AB228697)

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or ab228697. The precipitated DNA was detected by PCR with primer set targeting to CCND2.

Supplier Data

Western blot - Anti-KMT6 / EZH2 antibody (AB228697)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-KMT6 / EZH2 antibody (ab228697) at 1/1000 dilution

Lane 1:

NCI-H1299 (human lung carcinoma cell line) whole cell lysate at 30 µg

Lane 2:

HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg

Lane 3:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 85 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ChIP, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human EZH2. The exact immunogen used to generate this antibody is proprietary information.

Q15910

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KMT6 also known as EZH2 (Enhancer of Zeste Homolog 2) is a protein that functions as a histone methyltransferase. It catalyzes the methylation of histone H3 on lysine 27 (H3K27) a modification associated with gene silencing. EZH2 is a component of the Polycomb Repressive Complex 2 (PRC2) which plays an important role in chromatin remodeling and transcriptional repression. The EZH2 protein has a molecular weight of approximately 87 kDa and its gene is located on chromosome 7. In terms of expression EZH2 is found in various tissues but is notably abundant in growing and dividing cells.

Biological function summary

EZH2 plays a central role in regulating gene expression during development and differentiation. It is a core component of the PRC2 complex which includes other proteins such as EED and SUZ12. Through its catalytic activity EZH2 contributes to the maintenance of the transcriptionally repressed state of genes influencing processes such as cell identity and proliferation. In many cell types EZH2-mediated gene silencing is necessary for normal development and dysregulation can lead to aberrant cellular processes.

Pathways

EZH2 participates in key regulatory networks notably the Wnt signaling pathway and the p53 pathway. Within the Wnt pathway EZH2's activity contributes to controlling gene expression that influences cellular differentiation and proliferation. Meanwhile interaction with the p53 pathway can impact the cellular response to stress and DNA damage highlighting EZH2’s regulatory dimension. Other proteins like β-catenin in the Wnt pathway interact with EZH2 reflecting its integration in these complex signaling networks.

EZH2 has been linked to cancer and other proliferative diseases. Overexpression or mutation of EZH2 is frequently observed in cancers such as prostate and breast cancer where it contributes to unchecked cell growth and tumor progression. In these contexts proteins like p53 can interact abnormally with EZH2 leading to failure in tumor suppression mechanisms. Additionally deregulated EZH2 activity is implicated in certain developmental disorders highlighting its broader impact on cellular and organismal homeostasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalytic subunit of the PRC2/EED-EZH2 complex, a Polycomb group (PcG) complex that methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene (PubMed : 14532106, PubMed : 15225548, PubMed : 15385962, PubMed : 16618801, PubMed : 16936726, PubMed : 17344414, PubMed : 22323599, PubMed : 24474760, PubMed : 26581166, PubMed : 30026490, PubMed : 30923826). Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively (PubMed : 15231737, PubMed : 17210787, PubMed : 18285464, PubMed : 22323599, PubMed : 30923826). Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2 (PubMed : 22323599, PubMed : 30923826). Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation (PubMed : 19026781). The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (PubMed : 16357870, PubMed : 17200670). Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes (PubMed : 16179254, PubMed : 18086877, PubMed : 20935635). EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA (PubMed : 23063525). Regulates the circadian clock via histone methylation at the promoter of the circadian genes (PubMed : 16717091). Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription (By similarity).

See full target information EZH2

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical and translational hepatology 11:97-109 PubMed36406327

2022

MALAT1-mediated EZH2 Recruitment to the GFER Promoter Region Curbs Normal Hepatocyte Proliferation in Acute Liver Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Li Chen,Xintong Kang,Xiujuan Meng,Liang Huang,Yiting Du,Yilan Zeng,Chunfeng Liao

Cell death & disease 13:661 PubMed35906216

2022

LncRNA ANRIL mediates endothelial dysfunction through BDNF downregulation in chronic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Su,Bing Liu,Huimin Chen,Tingwei Zhang,Tongtong Huang,Yue Liu,Cheng Wang,Qiqi Ma,Qianhui Wang,Zhimei Lv,Rong Wang

Cell death discovery 8:274 PubMed35668081

2022

SMYD2 aggravates gastrointestinal stromal tumor via upregulation of EZH2 and downregulation of TET1.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Ji,Xiaofeng Xu,Cong Long,Jianjiang Wang,Li Ding,Zhizhong Zheng,Huiping Wu,Liu Yang,Lan Tao,Feng Gao

Communications biology 4:1207 PubMed34675360

2021

Speckles and paraspeckles coordinate to regulate HSV-1 genes transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Li,Ziqiang Wang

Oral diseases 29:880-891 PubMed34614259

2021

EZH2-mediated SLC7A11 upregulation via miR-125b-5p represses ferroptosis of TSCC.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Yu,Hesham MohamedAl-Sharani,Bin Zhang

Cell death & disease 12:819 PubMed34462420

2021

Silencing of O-linked N-acetylglucosamine transferase ameliorates hypercalcemia-induced neurotoxicity in renal failure by regulating EZH2/KLF2/CXCL1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yaochen Cao,Xin Chen,Hongming Sun

Frontiers in cell and developmental biology 9:658720 PubMed34422795

2021

Sevoflurane Inhibits Traumatic Brain Injury-Induced Neuron Apoptosis EZH2-Downregulated KLF4/p38 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongyu Wang,Juan Li,Anqi Wang,Zhaoyang Wang,Junmin Wang,Jingjing Yuan,Xin Wei,Fei Xing,Wei Zhang,Na Xing

Molecular medicine reports 24: PubMed34036375

2021

lncRNA TUG1 inhibits the cancer stem cell‑like properties of temozolomide‑resistant glioma cells by interacting with EZH2.

Applications

Unspecified application

Species

Unspecified reactive species

Yiqiang Cao,Wenying Chai,Yonggang Wang,Dang Tang,Dongchuan Shao,Hai Song,Jiang Long

Molecular cancer 20:77 PubMed34006303

2021

KDM6A-ARHGDIB axis blocks metastasis of bladder cancer by inhibiting Rac1.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Liu,Jianfeng Cui,Yajing Zhao,Xiaochen Liu,Lipeng Chen,Yangyang Xia,Yong Wang,Shouzhen Chen,Shuna Sun,Benkang Shi,Yongxin Zou

Cell biology international 44:1544-1555 PubMed32198816

2020

Yin Yang-1 suppresses CD40 ligand-CD40 signaling-mediated anti-inflammatory cytokine interleukin-10 expression in pulmonary adventitial fibroblasts by promoting histone H3 tri-methylation at lysine 27 modification on interleukin-10 promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Yun Pan,Jin-Xiu Yang,Yi-Fei Xu,Wei Mao

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Product promise

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Anti-KMT6 / EZH2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-KMT6 / EZH2 antibody (AB228697)

ChIP - Anti-KMT6 / EZH2 antibody (AB228697)

Western blot - Anti-KMT6 / EZH2 antibody (AB228697)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (16)

Product promise