Anti-KMT6 / EZH2 antibody - ChIP Grade

• ChIP-seq

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ChIP-sequencing - Anti-KMT6 / EZH2 antibody - ChIP Grade (AB195409)

ChIPseq results obtained with ab195409 directed against KMT6 / EZH2.

ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of ab195409. The IP'd DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. The figure shows the peak distribution along the short arm and a 6 Mb region containing several enriched regions of human chromosome 3 (figure A and B, respectively), and in two genomic regions containing the MYT1 gene on chromosome 20 and the HOX cluster on chromosome 7 (figure C and D).

• ChIP

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ChIP - Anti-KMT6 / EZH2 antibody - ChIP Grade (AB195409)

ChIP results obtained with ab195409 directed against KMT6 / EZH2.

ChIP assays were performed using K562 cells, ab195409 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for MYT1 and HOXA9, used as positive control targets, and for the coding regions of the active CCT5 and EIF2S3 genes, used as negative controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

• WB

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Western blot - Anti-KMT6 / EZH2 antibody - ChIP Grade (AB195409)

The antiody was diluted in TBS-Tween containing 5% skimmed milk.

All lanes:

Western blot - Anti-KMT6 / EZH2 antibody - ChIP Grade (ab195409) at 1/1000 dilution

All lanes:

HeLa nuclear extract at 40 µg

Predicted band size: 85 kDa

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