Rabbit Polyclonal KMT6 / EZH2 antibody. Suitable for ChIP, WB, ChIP-seq and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Ezh2 aa 1-350.
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- Cell death discovery 8:1032022LncRNA MEG3 up-regulates SIRT6 by ubiquitinating EZH2 and alleviates nonalcoholic fatty liver disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongmei Zou,Liang Liu,Yinzhen Zeng,Huanhuan Wang,Dongling Dai,Mingguo XuPubMed 35256601
- Journal of microbiology and biotechnology 31:1358-13652021LINC01232 Promotes Gastric Cancer Proliferation through Interacting with EZH2 to Inhibit the Transcription of KLF2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Liu,Zhen Li,Guohua Yu,Ting Wang,Guimei Qu,Yunhui WangPubMed 34409953
- Frontiers in cell and developmental biology 8:5528222020LncRNA MAGI2-AS3 Overexpression Sensitizes Esophageal Cancer Cells to Irradiation Through Down-Regulation of HOXB7 EZH2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenfang Cheng et. al.PubMed 33330444
- Cancer cell international 20:5512020EZH2 promotes the expression of LPA1 by mediating microRNA-139 promoter methylation to accelerate the development of ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongbo Wu et. al.PubMed 33292225
- Molecular oncology 14:3211-32332020Long noncoding RNA LINC01578 drives colon cancer metastasis through a positive feedback loop with the NF-κB/YY1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Liu et. al.PubMed 33040438
- Nature communications 11:36752020Multi-faceted epigenetic dysregulation of gene expression promotes esophageal squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Cao,Hayan Lee,Wei Wu,Aubhishek Zaman,Sean McCorkle,Ming Yan,Justin Chen,Qinghe Xing,Nasa Sinnott-Armstrong,Hongen Xu,M Reza Sailani,Wenxue Tang,Yuanbo Cui,Jia Liu,Hongyan Guan,Pengju Lv,Xiaoyan Sun,Lei Sun,Pengli Han,Yanan Lou,Jing Chang,Jinwu Wang,Yuchi Gao,Jiancheng Guo,Gundolf Schenk,Alan Hunter Shain,Fred G Biddle,Eric Collisson,Michael Snyder,Trever G BivonaPubMed 32699215
- Cancer cell international 20:1162020Long non-coding RNA GAS5 accelerates oxidative stress in melanoma cells by rescuing EZH2-mediated CDKN1C downregulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Xu et. al.PubMed 32308561
- Cell reports 29:1800-1811.e62019A Functional Link between Nuclear RNA Decay and Transcriptional Control Mediated by the Polycomb Repressive Complex 2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWilliam Garland,Itys Comet,Mengjun Wu,Aliaksandra Radzisheuskaya,Leonor Rib,Kristoffer Vitting-Seerup,Marta Lloret-Llinares,Albin Sandelin,Kristian Helin,Torben Heick JensenPubMed 31722198
- Theranostics 9:4717-47292019CDYL promotes the chemoresistance of small cell lung cancer by regulating H3K27 trimethylation at the CDKN1C promoter.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhengang Qiu et. al.PubMed 31367252
- Oncology letters 17:103-1122019Downregulation of long noncoding RNA FENDRR predicts poor prognosis in renal cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWang He,Guangzheng Zhong,Pei Wang,Chun Jiang,Ning Jiang,Jian HuangPubMed 30655744
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Proclin 300, 0.05% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Mouse Ezh2 aa 1-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q61188
Consider this alternative
Reactivity data
Select an application| ChIP | WB | ChIP-seq | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Cynomolgus monkey | Predicted | Predicted | Predicted |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Use 2 μg/ChIP for chromatin prepared from 4x106 cells. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cynomolgus monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cynomolgus monkey | Dilution info - | Notes - |
ChIP-seq
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Use 2 μg/ChIP for chromatin prepared from 4x106 cells |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cynomolgus monkey | Dilution info - | Notes - |
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Target data
Function
Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2 (PubMed:22323599, PubMed:30923826). Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.
Alternative names
KMT6, EZH2, Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6
Recommended products
Rabbit Polyclonal KMT6 / EZH2 antibody. Suitable for ChIP, WB, ChIP-seq and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Ezh2 aa 1-350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Proclin 300, 0.05% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Mouse Ezh2 aa 1-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q61188
- Purification technique
- Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KMT6 also known as EZH2 (Enhancer of Zeste Homolog 2) is a protein that functions as a histone methyltransferase. It catalyzes the methylation of histone H3 on lysine 27 (H3K27) a modification associated with gene silencing. EZH2 is a component of the Polycomb Repressive Complex 2 (PRC2) which plays an important role in chromatin remodeling and transcriptional repression. The EZH2 protein has a molecular weight of approximately 87 kDa and its gene is located on chromosome 7. In terms of expression EZH2 is found in various tissues but is notably abundant in growing and dividing cells.
- Biological function summary
EZH2 plays a central role in regulating gene expression during development and differentiation. It is a core component of the PRC2 complex which includes other proteins such as EED and SUZ12. Through its catalytic activity EZH2 contributes to the maintenance of the transcriptionally repressed state of genes influencing processes such as cell identity and proliferation. In many cell types EZH2-mediated gene silencing is necessary for normal development and dysregulation can lead to aberrant cellular processes.
- Pathways
EZH2 participates in key regulatory networks notably the Wnt signaling pathway and the p53 pathway. Within the Wnt pathway EZH2's activity contributes to controlling gene expression that influences cellular differentiation and proliferation. Meanwhile interaction with the p53 pathway can impact the cellular response to stress and DNA damage highlighting EZH2’s regulatory dimension. Other proteins like β-catenin in the Wnt pathway interact with EZH2 reflecting its integration in these complex signaling networks.
- Associated diseases and disorders
EZH2 has been linked to cancer and other proliferative diseases. Overexpression or mutation of EZH2 is frequently observed in cancers such as prostate and breast cancer where it contributes to unchecked cell growth and tumor progression. In these contexts proteins like p53 can interact abnormally with EZH2 leading to failure in tumor suppression mechanisms. Additionally deregulated EZH2 activity is implicated in certain developmental disorders highlighting its broader impact on cellular and organismal homeostasis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
ChIP - Anti-KMT6 / EZH2 antibody - ChIP Grade (ab195409)
ChIP results obtained with ab195409 directed against KMT6 / EZH2.
ChIP assays were performed using K562 cells, ab195409 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for MYT1 and HOXA9, used as positive control targets, and for the coding regions of the active CCT5 and EIF2S3 genes, used as negative controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ChIP-sequencing - Anti-KMT6 / EZH2 antibody - ChIP Grade (ab195409)
ChIPseq results obtained with ab195409 directed against KMT6 / EZH2.
ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of ab195409. The IP'd DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. The figure shows the peak distribution along the short arm and a 6 Mb region containing several enriched regions of human chromosome 3 (figure A and B, respectively), and in two genomic regions containing the MYT1 gene on chromosome 20 and the HOX cluster on chromosome 7 (figure C and D).
Western blot - Anti-KMT6 / EZH2 antibody - ChIP Grade (ab195409)
The antiody was diluted in TBS-Tween containing 5% skimmed milk.
All lanes: Western blot - Anti-KMT6 / EZH2 antibody - ChIP Grade (ab195409) at 1/1000 dilution
All lanes: HeLa nuclear extract at 40 µg
Predicted band size: 85 kDa
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