AB150433

Anti-KMT6 / EZH2 antibody [EPR9307]

RabMAb
Recombinant
KO Validated
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal KMT6 / EZH2 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

KMT6, EZH2, Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6

2 Images

Lab

Western blot - Anti-KMT6 / EZH2 antibody [EPR9307] (AB150433)

Lane 1 : Wild-type HAP1 cell lysate (40 μg)
Lane 2 : KMT6 / EZH2 knockout HAP1 cell lysate (40 μg)
Lane 3 : HEK293 cell lysate (20 μg)
Lanes 1 - 3 : Merged signal (red and green). Green - ab150433 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab150433 was shown to recognize KMT6 / EZH2 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when KMT6 / EZH2 knockout samples were examined. Wild-type and KMT6 / EZH2 knockout samples were subjected to SDS-PAGE. ab150433 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 dilution respectively and incubated overnight at 4^oC. Blots were developed with IRDye^® 800CW Goat anti-Rabbit IgG (H + L) (ab216773)
and IRDye^® 680 Goat anti-Mouse IgG (H + L) (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-KMT6 / EZH2 antibody [EPR9307] (ab150433)

Predicted band size: 85 kDa

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Western blot - Anti-KMT6 / EZH2 antibody [EPR9307] (AB150433)

All lanes:

Western blot - Anti-KMT6 / EZH2 antibody [EPR9307] (ab150433) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 85 kDa

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Carrier free

Anti-KMT6 / EZH2 antibody [EPR9307] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9307

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KMT6 also known as EZH2 (Enhancer of Zeste Homolog 2) is a protein that functions as a histone methyltransferase. It catalyzes the methylation of histone H3 on lysine 27 (H3K27) a modification associated with gene silencing. EZH2 is a component of the Polycomb Repressive Complex 2 (PRC2) which plays an important role in chromatin remodeling and transcriptional repression. The EZH2 protein has a molecular weight of approximately 87 kDa and its gene is located on chromosome 7. In terms of expression EZH2 is found in various tissues but is notably abundant in growing and dividing cells.

Biological function summary

EZH2 plays a central role in regulating gene expression during development and differentiation. It is a core component of the PRC2 complex which includes other proteins such as EED and SUZ12. Through its catalytic activity EZH2 contributes to the maintenance of the transcriptionally repressed state of genes influencing processes such as cell identity and proliferation. In many cell types EZH2-mediated gene silencing is necessary for normal development and dysregulation can lead to aberrant cellular processes.

Pathways

EZH2 participates in key regulatory networks notably the Wnt signaling pathway and the p53 pathway. Within the Wnt pathway EZH2's activity contributes to controlling gene expression that influences cellular differentiation and proliferation. Meanwhile interaction with the p53 pathway can impact the cellular response to stress and DNA damage highlighting EZH2’s regulatory dimension. Other proteins like β-catenin in the Wnt pathway interact with EZH2 reflecting its integration in these complex signaling networks.

EZH2 has been linked to cancer and other proliferative diseases. Overexpression or mutation of EZH2 is frequently observed in cancers such as prostate and breast cancer where it contributes to unchecked cell growth and tumor progression. In these contexts proteins like p53 can interact abnormally with EZH2 leading to failure in tumor suppression mechanisms. Additionally deregulated EZH2 activity is implicated in certain developmental disorders highlighting its broader impact on cellular and organismal homeostasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2 (PubMed : 22323599, PubMed : 30923826). Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.

See full target information EZH2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:619795 PubMed34409024

2021

EZH2/EHMT2 Histone Methyltransferases Inhibit the Transcription of DLX5 and Promote the Transformation of Myelodysplastic Syndrome to Acute Myeloid Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuanzhen Zheng,Ling Li,Guoxia Li,Yaofang Zhang,Chunxia Dong,Fanggang Ren,Wenliang Chen,Yanping Ma

Theranostics 9:4717-4729 PubMed31367252

2019

CDYL promotes the chemoresistance of small cell lung cancer by regulating H3K27 trimethylation at the CDKN1C promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengang Qiu,Weiliang Zhu,Hui Meng,Lihua Tong,Xi Li,Peng Luo,Lilan Yi,Xiaoli Zhang,Linlang Guo,Ting Wei,Jian Zhang

Journal of neuro-oncology 144:137-146 PubMed31214915

2019

Evaluation of EZH2 expression, BRAF V600E mutation, and CDKN2A/B deletions in epithelioid glioblastoma and anaplastic pleomorphic xanthoastrocytoma.

Applications

Unspecified application

Species

Unspecified reactive species

Junmei Wang,Zhaoxia Liu,Yun Cui,Yuqing Liu,Jingyi Fang,Li Xu,Yanjiao He,Jiang Du,Yujin Su,Wanjing Zou,Zuolin Xu,Guilin Li

Experimental and therapeutic medicine 16:1663-1670 PubMed30186385

2018

MicroRNA-101-3p inhibits proliferation in retinoblastoma cells by targeting EZH2 and HDAC9.

Applications

Unspecified application

Species

Unspecified reactive species

Qifang Jin,Wenfeng He,Leifeng Chen,Yang Yang,Ke Shi,Zhipeng You

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KMT6 / EZH2 antibody [EPR9307]

Western blot - Anti-KMT6 / EZH2 antibody [EPR9307] (AB150433)

Western blot - Anti-KMT6 / EZH2 antibody [EPR9307] (AB150433)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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