Rabbit Recombinant Monoclonal KMT6 / EZH2 antibody. C-terminal. Suitable for ChIC/CUT&RUN-seq, IP, Flow Cyt (Intra), WB, IHC-P and reacts with Human, Mouse samples. Cited in 5 publications.
IgG
Rabbit
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Liquid
Monoclonal
ChIC/CUT&RUN-seq | IP | Flow Cyt (Intra) | WB | IHC-P | |
---|---|---|---|---|---|
Human | Tested | Tested | Tested | Tested | Tested |
Mouse | Expected | Expected | Expected | Tested | Expected |
Rat | Predicted | Predicted | Predicted | Predicted | Predicted |
Monkey | Predicted | Predicted | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 5 µg | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/20 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 | Notes Primary antibody incubation for 30 minutes at 4°C. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/25 | Notes Primary antibody incubation for 1 hour at room temperature. |
Species Human | Dilution info 1/25 | Notes Primary antibody incubation for 1 hour at room temperature. |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat mediated antigen retrieval with EDTA buffer before commencing with IHC staining protocol. Primary antibody incubation for 30 minutes at room temperature. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Monkey | Dilution info - | Notes - |
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Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2 (PubMed:22323599, PubMed:30923826). Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.
KMT6, EZH2, Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6
Rabbit Recombinant Monoclonal KMT6 / EZH2 antibody. C-terminal. Suitable for ChIC/CUT&RUN-seq, IP, Flow Cyt (Intra), WB, IHC-P and reacts with Human, Mouse samples. Cited in 5 publications.
IgG
Rabbit
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Liquid
Monoclonal
SP129
Affinity purification Protein A/G
Purified from TCS by protein A/G.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
This supplementary information is collated from multiple sources and compiled automatically.
KMT6 also known as EZH2 (Enhancer of Zeste Homolog 2) is a protein that functions as a histone methyltransferase. It catalyzes the methylation of histone H3 on lysine 27 (H3K27) a modification associated with gene silencing. EZH2 is a component of the Polycomb Repressive Complex 2 (PRC2) which plays an important role in chromatin remodeling and transcriptional repression. The EZH2 protein has a molecular weight of approximately 87 kDa and its gene is located on chromosome 7. In terms of expression EZH2 is found in various tissues but is notably abundant in growing and dividing cells.
EZH2 plays a central role in regulating gene expression during development and differentiation. It is a core component of the PRC2 complex which includes other proteins such as EED and SUZ12. Through its catalytic activity EZH2 contributes to the maintenance of the transcriptionally repressed state of genes influencing processes such as cell identity and proliferation. In many cell types EZH2-mediated gene silencing is necessary for normal development and dysregulation can lead to aberrant cellular processes.
EZH2 participates in key regulatory networks notably the Wnt signaling pathway and the p53 pathway. Within the Wnt pathway EZH2's activity contributes to controlling gene expression that influences cellular differentiation and proliferation. Meanwhile interaction with the p53 pathway can impact the cellular response to stress and DNA damage highlighting EZH2’s regulatory dimension. Other proteins like β-catenin in the Wnt pathway interact with EZH2 reflecting its integration in these complex signaling networks.
EZH2 has been linked to cancer and other proliferative diseases. Overexpression or mutation of EZH2 is frequently observed in cancers such as prostate and breast cancer where it contributes to unchecked cell growth and tumor progression. In these contexts proteins like p53 can interact abnormally with EZH2 leading to failure in tumor suppression mechanisms. Additionally deregulated EZH2 activity is implicated in certain developmental disorders highlighting its broader impact on cellular and organismal homeostasis.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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ab227648 (purified) at 1/20 dilution (20 μg/ml) immunoprecipitating KMT6 in HEK-293 whole cell lysate.
Lane 1 (input): HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10μg
Lane 2 (+): ab227648 & HEK-293 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab227648 in HEK-293 whole cell lysate
For western blotting, ab227648 (purified at 1/500 dilution (0.632 μg/ml) and veriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution were used.
Blocking and diluting buffer: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)
Predicted band size: 85 kDa
Blocking/Diluting buffer and concentration: 5% NFDM /TBST
All lanes: Western blot - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648) at 0.316 µg/mL
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate at 20 µg
Lane 2: NIH/3T3 (Mouse embryonic fibroblast) cell lysate at 20 µg
Lane 3: Mouse brain tissue lysate at 20 µg
Lane 4: HEK-293 (Human embryonic kidney epithelial cell) cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 0.05 µg/mL
Predicted band size: 85 kDa
Observed band size: 85 kDa
Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling KMT6 / EZH2 with ab227648 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue).
Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for KMT6 / EZH2 using ab227648 at 1/100 dilution in immunohistochemical analysis.
All lanes: Western blot - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648) at 1/25 dilution
All lanes: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Predicted band size: 20 kDa, 85 kDa
Observed band size: 22 kDa
ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2 x 10^5 K-562 (Human chronic myelogenous leukemia lymphoblast) cells and 5 µg of ab227648 [SP129]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
Additional screenshots of mapped reads can be found in the Protocol booklet in the Support and downloads section.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com