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AB227648

Anti-KMT6 / EZH2 antibody [SP129]

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(5 Publications)

Rabbit Recombinant Monoclonal KMT6 / EZH2 antibody. C-terminal. Suitable for ChIC/CUT&RUN-seq, IP, Flow Cyt (Intra), WB, IHC-P and reacts with Human, Mouse samples. Cited in 5 publications.

View Alternative Names

KMT6, EZH2, Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6 / EZH2 antibody [SP129] (AB227648)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6 / EZH2 antibody [SP129] (AB227648)

Formalin-fixed paraffin-embedded human prostate cancer tissue stained for KMT6 / EZH2 using ab227648 at 1/100 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-KMT6 / EZH2 antibody [SP129] (AB227648)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-KMT6 / EZH2 antibody [SP129] (AB227648)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling KMT6 / EZH2 with ab227648 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue).

Western blot - Anti-KMT6 / EZH2 antibody [SP129] (AB227648)
  • WB

Lab

Western blot - Anti-KMT6 / EZH2 antibody [SP129] (AB227648)

Blocking/Diluting buffer and concentration : 5% NFDM /TBST

All lanes:

Western blot - Anti-KMT6 / EZH2 antibody [SP129] (ab227648) at 0.316 µg/mL

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate at 20 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast) cell lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Lane 4:

HEK-293 (Human embryonic kidney epithelial cell) cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 0.05 µg/mL

Predicted band size: 85 kDa

Observed band size: 85 kDa

false

ChIC/CUT&RUN sequencing - Anti-KMT6 / EZH2 antibody [SP129] (AB227648)
  • ChIC/CUT&RUN-seq

Supplier Data

ChIC/CUT&RUN sequencing - Anti-KMT6 / EZH2 antibody [SP129] (AB227648)

ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2 x 10^5 K-562 (Human chronic myelogenous leukemia lymphoblast) cells and 5 µg of ab227648 [SP129]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. Additional screenshots of mapped reads can be found in the Protocol booklet in the Product Protocol section. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

Immunoprecipitation - Anti-KMT6 / EZH2 antibody [SP129] (AB227648)
  • IP

Lab

Immunoprecipitation - Anti-KMT6 / EZH2 antibody [SP129] (AB227648)

ab227648 (purified) at 1/20 dilution (20 μg/ml) immunoprecipitating KMT6 in HEK-293 whole cell lysate.

Lane 1 (input) : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab227648 & HEK-293 whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab227648 in HEK-293 whole cell lysate

For western blotting, ab227648 (purified at 1/500 dilution (0.632 μg/ml) and veriBlot for IP secondary antibody (HRP) (ab131366) at 1/1000 dilution were used.
Blocking and diluting buffer : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-KMT6 / EZH2 antibody [SP129] (ab227648)

Predicted band size: 85 kDa

false

Western blot - Anti-KMT6 / EZH2 antibody [SP129] (AB227648)
  • WB

Supplier Data

Western blot - Anti-KMT6 / EZH2 antibody [SP129] (AB227648)

All lanes:

Western blot - Anti-KMT6 / EZH2 antibody [SP129] (ab227648) at 1/25 dilution

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Predicted band size: 20 kDa,85 kDa

Observed band size: 22 kDa

false

  • Carrier free

    Anti-KMT6 / EZH2 antibody [SP129] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-KMT6 / EZH2 antibody [SP129]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-KMT6 / EZH2 antibody [SP129]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-KMT6 / EZH2 antibody [SP129]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-KMT6 / EZH2 antibody [SP129]

  • 578 PE

    PE Anti-KMT6 / EZH2 antibody [SP129]

  • 660 APC

    APC Anti-KMT6 / EZH2 antibody [SP129]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-KMT6 / EZH2 antibody [SP129]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP129

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ChIC/CUT&RUN-seq, IP, IHC-P, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChICCUTRUNseq" : {"fullname" : "ChIC/CUT&RUN sequencing", "shortname":"ChIC/CUT&RUN-seq"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChICCUTRUNseq-species-checked": "testedAndGuaranteed", "ChICCUTRUNseq-species-dilution-info": "5 µg", "ChICCUTRUNseq-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p>Primary antibody incubation for 30 minutes at 4°C.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/25", "WB-species-notes": "<p>Primary antibody incubation for 1 hour at room temperature.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval with EDTA buffer before commencing with IHC staining protocol.</p><p>Primary antibody incubation for 30 minutes at room temperature.</p>" }, "Mouse": { "ChICCUTRUNseq-species-checked": "guaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/25", "WB-species-notes": "<p>Primary antibody incubation for 1 hour at room temperature.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ChICCUTRUNseq-species-checked": "predicted", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "ChICCUTRUNseq-species-checked": "predicted", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by protein A/G.
Storage buffer
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KMT6 also known as EZH2 (Enhancer of Zeste Homolog 2) is a protein that functions as a histone methyltransferase. It catalyzes the methylation of histone H3 on lysine 27 (H3K27) a modification associated with gene silencing. EZH2 is a component of the Polycomb Repressive Complex 2 (PRC2) which plays an important role in chromatin remodeling and transcriptional repression. The EZH2 protein has a molecular weight of approximately 87 kDa and its gene is located on chromosome 7. In terms of expression EZH2 is found in various tissues but is notably abundant in growing and dividing cells.
Biological function summary

EZH2 plays a central role in regulating gene expression during development and differentiation. It is a core component of the PRC2 complex which includes other proteins such as EED and SUZ12. Through its catalytic activity EZH2 contributes to the maintenance of the transcriptionally repressed state of genes influencing processes such as cell identity and proliferation. In many cell types EZH2-mediated gene silencing is necessary for normal development and dysregulation can lead to aberrant cellular processes.

Pathways

EZH2 participates in key regulatory networks notably the Wnt signaling pathway and the p53 pathway. Within the Wnt pathway EZH2's activity contributes to controlling gene expression that influences cellular differentiation and proliferation. Meanwhile interaction with the p53 pathway can impact the cellular response to stress and DNA damage highlighting EZH2’s regulatory dimension. Other proteins like β-catenin in the Wnt pathway interact with EZH2 reflecting its integration in these complex signaling networks.

EZH2 has been linked to cancer and other proliferative diseases. Overexpression or mutation of EZH2 is frequently observed in cancers such as prostate and breast cancer where it contributes to unchecked cell growth and tumor progression. In these contexts proteins like p53 can interact abnormally with EZH2 leading to failure in tumor suppression mechanisms. Additionally deregulated EZH2 activity is implicated in certain developmental disorders highlighting its broader impact on cellular and organismal homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2 (PubMed : 22323599, PubMed : 30923826). Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.
See full target information EZH2
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 13:657 PubMed35902569

2022

m6A methyltransferase METTL3-induced lncRNA SNHG17 promotes lung adenocarcinoma gefitinib resistance by epigenetically repressing LATS2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Zhang,Shao-Qiang Wang,Li Wang,Hang Lin,Jie-Bo Zhu,Ri Chen,Lin-Feng Li,Yuan-Da Cheng,Chao-Jun Duan,Chun-Fang Zhang

Molecular therapy. Nucleic acids 25:116-126 PubMed34401209

2021

lncRNA H19 facilitates the proliferation and differentiation of human dental pulp stem cells via EZH2-dependent LATS1 methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Du,Xiaoming Shi,Aizhong Guan

Cell death & disease 12:188 PubMed33589600

2021

SChLAP1 promotes prostate cancer development through interacting with EZH2 to mediate promoter methylation modification of multiple miRNAs of chromosome 5 with a DNMT3a-feedback loop.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Huang,Yuxin Tang

Journal of cellular and molecular medicine 24:11858-11873 PubMed32885590

2020

The long non-coding RNA SNHG1 promotes bladder cancer progression by interacting with miR-143-3p and EZH2.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Xiang,Lei Lyu,Tao Huang,Fuxin Zheng,Jingdong Yuan,Chuanhua Zhang,Guosong Jiang

Urologic oncology 36:343.e1-343.e8 PubMed29748098

2018

Multi-institutional evaluation of the prognostic significance of EZH2 expression in high-grade upper tract urothelial carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Nirmish Singla,Laura-Maria Krabbe,Ahmet M Aydin,Vandana Panwar,Solomon L Woldu,Yuval Freifeld,Christopher G Wood,Jose A Karam,Alon Z Weizer,Jay D Raman,Mesut Remzi,Nathalie Rioux-Leclercq,Andrea Haitel,Marco Roscigno,Christian Bolenz,Karim Bensalah,Arthur I Sagalowsky,Shahrokh F Shariat,Yair Lotan,Aditya Bagrodia,Payal Kapur,Vitaly Margulis
View all publications
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chicCutRunSequencingBooklet
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