AB300568

Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (BSA and Azide free)

BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal KMT6 / EZH2 phospho T487 antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra), Dot and reacts with Human, Mouse, Rat, Synthetic peptide - Human samples.

View Alternative Names

KMT6, EZH2, Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (BSA and Azide free) (AB300568)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (BSA and Azide free) (AB300568)

This data was developed using ab300567, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling KMT6 / EZH2 (phos Thr487) with ab300567 at 1/2000 (0.221 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human tonsil without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab300567 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Flow Cytometry (Intracellular) - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (BSA and Azide free) (AB300568)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (BSA and Azide free) (AB300568)

This data was developed using ab300567, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) treated with 20µM nocodazole for 24 hours (Right) / Untreated control (Left) cells labelling KMT6 / EZH2 (phos Thr487) with ab300567 at 1/500 dilution (0.1ug)/ Left and Right (Red) compared with an isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (BSA and Azide free) (AB300568)

This data was developed using ab300567, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling KMT6 / EZH2 (phos Thr487) with ab300567 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (BSA and Azide free) (AB300568)

This data was developed using ab300567, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling KMT6 / EZH2 (phos Thr487) with ab300567 at 1/2000 (0.221 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat colon without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab300567 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (BSA and Azide free) (AB300568)

This data was developed using ab300567, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling KMT6 / EZH2 (phos Thr487) with ab300567 at 1/2000 (0.221 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse spleen without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab300567 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Supplier Data

Western blot - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (BSA and Azide free) (AB300568)

This data was developed using ab300567, the same antibody clone in a different buffer formulation. Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (<a href='/en-us/products/primary-antibodies/kmt6-ezh2-phospho-t487-antibody-epr24903-104-ab300567'>ab300567</a>) at 1/1000 dilution

Lane 1:

HeLa treated with 2mM thymidine for 16hours, and then 10nM nocodazole for 24 hours, whole cell lysate 10 µg (untreated membrane)

Lane 2:

HeLa treated with 2mM thymidine for 16hours, and then 10nM nocodazole for 24 hours, whole cell lysate 10 µg (phosphatase treated membrane)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 90 kDa

false

Exposure time: 26s

Supplier Data

Western blot - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (BSA and Azide free) (AB300568)

This data was developed using ab300567, the same antibody clone in a different buffer formulation. Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Lane 1:

Untreated HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 20 µg

Lane 2:

HeLa treated with 0.5uM nocodazole for 24 hours, whole cell lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 90 kDa

false

Exposure time: 70s

Supplier Data

Dot Blot - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (BSA and Azide free) (AB300568)

This data was developed using ab300567, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (<a href='/en-us/products/primary-antibodies/kmt6-ezh2-phospho-t487-antibody-epr24903-104-ab300567'>ab300567</a>) at 1/1000 dilution

Lane 1:

KMT6/EZH2 (phospho Thr487) peptide a

Lane 2:

KMT6/EZH2 (phospho Thr487) peptide b

Lane 3:

KMT6/EZH2 non-phospho peptide corresponding to the region of phospho peptides a and b

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

Unconjugated

Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24903-104

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

Dot, WB, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Synthetic peptide - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KMT6 also known as EZH2 (Enhancer of Zeste Homolog 2) is a protein that functions as a histone methyltransferase. It catalyzes the methylation of histone H3 on lysine 27 (H3K27) a modification associated with gene silencing. EZH2 is a component of the Polycomb Repressive Complex 2 (PRC2) which plays an important role in chromatin remodeling and transcriptional repression. The EZH2 protein has a molecular weight of approximately 87 kDa and its gene is located on chromosome 7. In terms of expression EZH2 is found in various tissues but is notably abundant in growing and dividing cells.

Biological function summary

EZH2 plays a central role in regulating gene expression during development and differentiation. It is a core component of the PRC2 complex which includes other proteins such as EED and SUZ12. Through its catalytic activity EZH2 contributes to the maintenance of the transcriptionally repressed state of genes influencing processes such as cell identity and proliferation. In many cell types EZH2-mediated gene silencing is necessary for normal development and dysregulation can lead to aberrant cellular processes.

Pathways

EZH2 participates in key regulatory networks notably the Wnt signaling pathway and the p53 pathway. Within the Wnt pathway EZH2's activity contributes to controlling gene expression that influences cellular differentiation and proliferation. Meanwhile interaction with the p53 pathway can impact the cellular response to stress and DNA damage highlighting EZH2’s regulatory dimension. Other proteins like β-catenin in the Wnt pathway interact with EZH2 reflecting its integration in these complex signaling networks.

EZH2 has been linked to cancer and other proliferative diseases. Overexpression or mutation of EZH2 is frequently observed in cancers such as prostate and breast cancer where it contributes to unchecked cell growth and tumor progression. In these contexts proteins like p53 can interact abnormally with EZH2 leading to failure in tumor suppression mechanisms. Additionally deregulated EZH2 activity is implicated in certain developmental disorders highlighting its broader impact on cellular and organismal homeostasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalytic subunit of the PRC2/EED-EZH2 complex, a Polycomb group (PcG) complex that methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene (PubMed : 14532106, PubMed : 15225548, PubMed : 15385962, PubMed : 16618801, PubMed : 16936726, PubMed : 17344414, PubMed : 22323599, PubMed : 24474760, PubMed : 26581166, PubMed : 30026490, PubMed : 30923826). Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively (PubMed : 15231737, PubMed : 17210787, PubMed : 18285464, PubMed : 22323599, PubMed : 30923826). Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2 (PubMed : 22323599, PubMed : 30923826). Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation (PubMed : 19026781). The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (PubMed : 16357870, PubMed : 17200670). Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes (PubMed : 16179254, PubMed : 18086877, PubMed : 20935635). EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA (PubMed : 23063525). Regulates the circadian clock via histone methylation at the promoter of the circadian genes (PubMed : 16717091). Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription (By similarity).

See full target information EZH2 phospho T487

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (BSA and Azide free)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (BSA and Azide free) (AB300568)

Flow Cytometry (Intracellular) - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (BSA and Azide free) (AB300568)

Flow Cytometry (Intracellular) - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (BSA and Azide free) (AB300568)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (BSA and Azide free) (AB300568)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (BSA and Azide free) (AB300568)

Western blot - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (BSA and Azide free) (AB300568)

Western blot - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (BSA and Azide free) (AB300568)

Dot Blot - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (BSA and Azide free) (AB300568)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise