AB6036

Anti-KPNA2 antibody

Name: Anti-KPNA2 antibody (ab6036) | Abcam
Brand: Abcam Limited
SKU: AB6036

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(5 Publications)

Goat Polyclonal KPNA2 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human KPNA2 aa 500 to C-terminus.

View Alternative Names

RCH1, SRP1, KPNA2, Importin subunit alpha-1, Karyopherin subunit alpha-2, RAG cohort protein 1, SRP1-alpha

4 Images

Flow Cytometry - Anti-KPNA2 antibody (AB6036)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-KPNA2 antibody (AB6036)

Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-KPNA2 antibody (AB6036)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-KPNA2 antibody (AB6036)

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and ER/Golgi staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KPNA2 antibody (AB6036)

Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Supplier Data

Western blot - Anti-KPNA2 antibody (AB6036)

Detected by chemiluminescence.

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Western blot - Anti-KPNA2 antibody (ab6036) at 0.1 µg/mL

Lanes 3 - 5:

Western blot - Anti-KPNA2 antibody (ab6036) at 0.03 µg/mL

Lane 1:

Jurkat cell lysate at 35 µg

Lane 2:

CaCo-2 cell lysate at 35 µg

Lane 3:

A549 cell lysate at 35 µg

Lane 4:

MCF7 at 35 µg

Lane 5:

KNRK cell lysate at 35 µg

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, ICC/IF, IHC-P, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human KPNA2 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P52292

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µg/mL", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03-0.1 µg/mL", "WB-species-notes": "A 1 hour primary incubation is recommended for this product.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03-0.1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

GenBank Accession Number – NP_002257.

The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KPNA2 also known as Importin subunit alpha-1 functions as a facilitator for nuclear protein import. It has a mass of about 58 kDa and resides mainly in the cytoplasm and nucleus. This protein binds with specific nuclear localization signals on cargo proteins and assists in their transport into the nucleus through the nuclear pore complex. KPNA2 expression levels vary between tissues but it often appears in higher amounts in proliferative tissues.

Biological function summary

KPNA2 plays an important role in cellular processes by forming a complex with importin beta aiding nuclear-cytoplasmic transport. This transport system is important for regulating gene expression and maintaining cellular homeostasis. KPNA2 influences the correct localization of many signaling and transcription factors impacting numerous cellular functions including cell cycle progression and DNA repair.

Pathways

KPNA2 is a critical player in the nuclear import pathway and intersects with the cell cycle regulation pathway. It is closely associated with proteins such as importin beta which together facilitate the translocation of proteins into the nucleus. KPNA2's function in importing various regulators ensures proper cell cycle progression and responds to cellular stress signals.

KPNA2 is linked to several cancers particularly breast and ovarian cancer. Its overexpression often correlates with poor prognosis in these cancers suggesting a role in tumor progression. KPNA2 interacts with oncogenic proteins like MYC and AURKA in these pathological states influencing tumor growth and survival mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1 (PubMed : 28991411, PubMed : 32130408, PubMed : 7604027, PubMed : 7754385). Binds specifically and directly to substrates containing either a simple or bipartite NLS motif (PubMed : 28991411, PubMed : 32130408, PubMed : 7604027, PubMed : 7754385). Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed : 7604027, PubMed : 7754385). At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediator of PR-DUB complex component BAP1 nuclear import; acts redundantly with KPNA1 and Transportin-1/TNPO1 (PubMed : 35446349).

See full target information KPNA2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of oncology 2022:9973519 PubMed36199790

2022

IRF2 Destabilizes Oncogenic KPNA2 to Modulate the Development of Osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shuchi Xia,Yiqun Ma

PLoS pathogens 18:e1010798 PubMed36007070

2022

An Arginine-Rich Motif in the ORF2 capsid protein regulates the hepatitis E virus lifecycle and interactions with the host cell.

Applications

Unspecified application

Species

Unspecified reactive species

Kévin Hervouet,Martin Ferrié,Maliki Ankavay,Claire Montpellier,Charline Camuzet,Virginie Alexandre,Aïcha Dembélé,Cécile Lecoeur,Arnold Thomas Foe,Peggy Bouquet,David Hot,Thibaut Vausselin,Jean-Michel Saliou,Sophie Salomé-Desnoulez,Alexandre Vandeputte,Laurent Marsollier,Priscille Brodin,Marlène Dreux,Yves Rouillé,Jean Dubuisson,Cécile-Marie Aliouat-Denis,Laurence Cocquerel

Cell reports 31:107549 PubMed32320654

2020

Cellular Importin-α3 Expression Dynamics in the Lung Regulate Antiviral Response Pathways against Influenza A Virus Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Swantje Thiele,Stephanie Stanelle-Bertram,Sebastian Beck,Nancy Mounogou Kouassi,Martin Zickler,Martin Müller,Berfin Tuku,Patricia Resa-Infante,Debby van Riel,Malik Alawi,Thomas Günther,Franziska Rother,Stefanie Hügel,Susanne Reimering,Alice McHardy,Adam Grundhoff,Wolfram Brune,Albert Osterhaus,Michael Bader,Enno Hartmann,Gülsah Gabriel

Journal of cell science 133: PubMed31932502

2020

Karyopherin enrichment at the nuclear pore complex attenuates Ran permeability.

Applications

Unspecified application

Species

Unspecified reactive species

Suncica Barbato,Larisa E Kapinos,Chantal Rencurel,Roderick Y H Lim

Journal of cell science 129:4025-4033 PubMed27609837

2016

RNA-binding protein HuR regulates nuclear import of protein.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhang,Amanda C Vreeland,Noa Noy

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KPNA2 antibody

Flow Cytometry - Anti-KPNA2 antibody (AB6036)

Immunocytochemistry/ Immunofluorescence - Anti-KPNA2 antibody (AB6036)

Immunocytochemistry/ Immunofluorescence - Anti-KPNA2 antibody (AB6036)

Western blot - Anti-KPNA2 antibody (AB6036)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise