Goat Polyclonal KPNA2 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human KPNA2 aa 500 to C-terminus.
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- Journal of oncology 2022:99735192022IRF2 Destabilizes Oncogenic KPNA2 to Modulate the Development of Osteosarcoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuchi Xia,Yiqun MaPubMed 36199790
- PLoS pathogens 18:e10107982022An Arginine-Rich Motif in the ORF2 capsid protein regulates the hepatitis E virus lifecycle and interactions with the host cell.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKévin Hervouet,Martin Ferrié,Maliki Ankavay,Claire Montpellier,Charline Camuzet,Virginie Alexandre,Aïcha Dembélé,Cécile Lecoeur,Arnold Thomas Foe,Peggy Bouquet,David Hot,Thibaut Vausselin,Jean-Michel Saliou,Sophie Salomé-Desnoulez,Alexandre Vandeputte,Laurent Marsollier,Priscille Brodin,Marlène Dreux,Yves Rouillé,Jean Dubuisson,Cécile-Marie Aliouat-Denis,Laurence CocquerelPubMed 36007070
- Cell reports 31:1075492020Cellular Importin-α3 Expression Dynamics in the Lung Regulate Antiviral Response Pathways against Influenza A Virus Infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSwantje Thiele,Stephanie Stanelle-Bertram,Sebastian Beck,Nancy Mounogou Kouassi,Martin Zickler,Martin Müller,Berfin Tuku,Patricia Resa-Infante,Debby van Riel,Malik Alawi,Thomas Günther,Franziska Rother,Stefanie Hügel,Susanne Reimering,Alice McHardy,Adam Grundhoff,Wolfram Brune,Albert Osterhaus,Michael Bader,Enno Hartmann,Gülsah GabrielPubMed 32320654
- Journal of cell science 133:2020Karyopherin enrichment at the nuclear pore complex attenuates Ran permeability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSuncica Barbato et. al.PubMed 31932502
- Journal of cell science 129:4025-40332016RNA-binding protein HuR regulates nuclear import of protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Zhang,Amanda C Vreeland,Noa NoyPubMed 27609837
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human KPNA2 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P52292
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Reactivity data
Select an application| Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted |
| Rat | Expected | Tested | Predicted | Expected |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.03-0.1 µg/mL | Notes A 1 hour primary incubation is recommended for this product. |
Species Rat | Dilution info 0.03-0.1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
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Target data
Function
Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1 (PubMed:28991411, PubMed:32130408, PubMed:7604027, PubMed:7754385). Binds specifically and directly to substrates containing either a simple or bipartite NLS motif (PubMed:28991411, PubMed:32130408, PubMed:7604027, PubMed:7754385). Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed:7604027, PubMed:7754385). At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediator of PR-DUB complex component BAP1 nuclear import; acts redundantly with KPNA1 and Transportin-1/TNPO1 (PubMed:35446349).
Alternative names
RCH1, SRP1, KPNA2, Importin subunit alpha-1, Karyopherin subunit alpha-2, RAG cohort protein 1, SRP1-alpha
Recommended products
Goat Polyclonal KPNA2 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human KPNA2 aa 500 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human KPNA2 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P52292
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
GenBank Accession Number – NP_002257.
The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KPNA2 also known as Importin subunit alpha-1 functions as a facilitator for nuclear protein import. It has a mass of about 58 kDa and resides mainly in the cytoplasm and nucleus. This protein binds with specific nuclear localization signals on cargo proteins and assists in their transport into the nucleus through the nuclear pore complex. KPNA2 expression levels vary between tissues but it often appears in higher amounts in proliferative tissues.
- Biological function summary
KPNA2 plays an important role in cellular processes by forming a complex with importin beta aiding nuclear-cytoplasmic transport. This transport system is important for regulating gene expression and maintaining cellular homeostasis. KPNA2 influences the correct localization of many signaling and transcription factors impacting numerous cellular functions including cell cycle progression and DNA repair.
- Pathways
KPNA2 is a critical player in the nuclear import pathway and intersects with the cell cycle regulation pathway. It is closely associated with proteins such as importin beta which together facilitate the translocation of proteins into the nucleus. KPNA2's function in importing various regulators ensures proper cell cycle progression and responds to cellular stress signals.
- Associated diseases and disorders
KPNA2 is linked to several cancers particularly breast and ovarian cancer. Its overexpression often correlates with poor prognosis in these cancers suggesting a role in tumor progression. KPNA2 interacts with oncogenic proteins like MYC and AURKA in these pathological states influencing tumor growth and survival mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunocytochemistry/ Immunofluorescence - Anti-KPNA2 antibody (ab6036)
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and ER/Golgi staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunocytochemistry/ Immunofluorescence - Anti-KPNA2 antibody (ab6036)
Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Western blot - Anti-KPNA2 antibody (ab6036)
Detected by chemiluminescence.
Lanes 1 - 2: Western blot - Anti-KPNA2 antibody (ab6036) at 0.1 µg/mL
Lanes 3 - 5: Western blot - Anti-KPNA2 antibody (ab6036) at 0.03 µg/mL
Lane 1: Jurkat cell lysate at 35 µg
Lane 2: CaCo-2 cell lysate at 35 µg
Lane 3: A549 cell lysate at 35 µg
Lane 4: MCF7 at 35 µg
Lane 5: KNRK cell lysate at 35 µg
Flow Cytometry - Anti-KPNA2 antibody (ab6036)
Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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