AB84440

Anti-KPNA2 antibody

Name: Anti-KPNA2 antibody (ab84440) | Abcam
Brand: Abcam Limited
SKU: AB84440
Price: 480 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(32 Publications)

Rabbit Polyclonal KPNA2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 32 publications. Immunogen corresponding to Synthetic Peptide within Human KPNA2 aa 450 to C-terminus.

View Alternative Names

RCH1, SRP1, KPNA2, Importin subunit alpha-1, Karyopherin subunit alpha-2, RAG cohort protein 1, SRP1-alpha

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA2 antibody (AB84440)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA2 antibody (AB84440)

Immunohistochemical analysis of KPNA2 in formalin fixed, paraffin embedded human bladder carcinoma tissue, using a 1/250 dilution of ab84440.
Detection : DAB staining.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA2 antibody (AB84440)

Immunohistochemical analysis of KPNA2 in formalin fixed, paraffin embedded human metastatic lymph node tissue, using a 1/250 dilution of ab84440.
Detection : DAB staining.

IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA2 antibody (AB84440)

Immunohistochemical analysis of Human ovarian germ cell tumour, staining KPNA2 with ab84440.

Antigen retrieval was performed by submerging the sample in citrate buffer (pH 6) and microwaving. The sections were blocked with 1% BSA before incubating with primary antibody (1/400). Staining was detected using DAB.

Image from He L et al., PLoS One. 2012;7(9):e42992. doi: 10.1371/journal.pone.0042992. Epub 2012 Sep 4. Fig 4.; doi:10.1371/journal.pone.0042992; September 4, 2012, PLoS ONE 7(9): e42992.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA2 antibody (AB84440)

Immunohistochemical analysis of KPNA2 in formalin fixed, paraffin embedded mouse squamous cell carcinoma tissue, using a 1/250 dilution of ab84440.
Detection : DAB staining.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA2 antibody (AB84440)

Immunohistochemical analysis of KPNA2 in formalin fixed, paraffin embedded mouse teratoma tissue, using a 1/250 dilution of ab84440.
Detection : DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human KPNA2 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P52292

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Concentration is optimized for IHC and not determined

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KPNA2 also known as Importin subunit alpha-1 functions as a facilitator for nuclear protein import. It has a mass of about 58 kDa and resides mainly in the cytoplasm and nucleus. This protein binds with specific nuclear localization signals on cargo proteins and assists in their transport into the nucleus through the nuclear pore complex. KPNA2 expression levels vary between tissues but it often appears in higher amounts in proliferative tissues.

Biological function summary

KPNA2 plays an important role in cellular processes by forming a complex with importin beta aiding nuclear-cytoplasmic transport. This transport system is important for regulating gene expression and maintaining cellular homeostasis. KPNA2 influences the correct localization of many signaling and transcription factors impacting numerous cellular functions including cell cycle progression and DNA repair.

Pathways

KPNA2 is a critical player in the nuclear import pathway and intersects with the cell cycle regulation pathway. It is closely associated with proteins such as importin beta which together facilitate the translocation of proteins into the nucleus. KPNA2's function in importing various regulators ensures proper cell cycle progression and responds to cellular stress signals.

KPNA2 is linked to several cancers particularly breast and ovarian cancer. Its overexpression often correlates with poor prognosis in these cancers suggesting a role in tumor progression. KPNA2 interacts with oncogenic proteins like MYC and AURKA in these pathological states influencing tumor growth and survival mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1 (PubMed : 28991411, PubMed : 32130408, PubMed : 7604027, PubMed : 7754385). Binds specifically and directly to substrates containing either a simple or bipartite NLS motif (PubMed : 28991411, PubMed : 32130408, PubMed : 7604027, PubMed : 7754385). Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed : 7604027, PubMed : 7754385). At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediator of PR-DUB complex component BAP1 nuclear import; acts redundantly with KPNA1 and Transportin-1/TNPO1 (PubMed : 35446349).

See full target information KPNA2

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Publications (32)

Recent publications for all applications. Explore the full list and refine your search

Cancer diagnosis & prognosis 5:1-7 PubMed39758242

2025

High Tumoral KPNA2 Expression Is a PotentialBiomarker for Poor Prognosis in Advanced Neuroblastoma Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Sayaka Otake,Kenjiro Ogushi,Gendensuren Dorjkhorloo,Takehiko Yokobori,Akira Nishi,Hiroyuki Kuwano,Hiroshi Saeki,Ken Shirabe

eLife 13: PubMed39495216

2024

Nuclear translocation of SIRT4 mediates deacetylation of U2AF2 to modulate renal fibrosis through alternative splicing-mediated upregulation of CCN2.

Applications

Unspecified application

Species

Unspecified reactive species

Guangyan Yang,Jiaqing Xiang,Xiaoxiao Yang,Xiaomai Liu,Yanchun Li,Lixing Li,Lin Kang,Zhen Liang,Shu Yang

EMBO molecular medicine 15:e15984 PubMed37792911

2023

Nuclear ERK1/2 signaling potentiation enhances neuroprotection and cognition via Importinα1/KPNA2.

Applications

Unspecified application

Species

Unspecified reactive species

Marzia Indrigo,Ilaria Morella,Daniel Orellana,Raffaele d'Isa,Alessandro Papale,Riccardo Parra,Antonia Gurgone,Daniela Lecca,Anna Cavaccini,Cezar M Tigaret,Alfredo Cagnotto,Kimberley Jones,Simon Brooks,Gian Michele Ratto,Nicholas D Allen,Mariah J Lelos,Silvia Middei,Maurizio Giustetto,Anna R Carta,Raffaella Tonini,Mario Salmona,Jeremy Hall,Kerrie Thomas,Riccardo Brambilla,Stefania Fasano

Histology and histopathology 38:1169-1178 PubMed36583484

2022

MiR-101-3p targets KPNA2 to inhibit the progression of lung squamous cell carcinoma cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Liangliang Dong,Hanliang Jiang,Ting Qiu,Yiming Xu,Enguo Chen,Aihua Huang,Kejing Ying

Cancers 14: PubMed35884546

2022

Identification and Characterization of Cancer-Associated Fibroblast Subpopulations in Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Daeseung Kim,Jeong Seon Kim,Inyoung Cheon,Seo Ree Kim,Sang Hoon Chun,Jae Jun Kim,Sieun Lee,Jung Sook Yoon,Soon Auck Hong,Hye Sung Won,Keunsoo Kang,Young-Ho Ahn,Yoon Ho Ko

Cancer research 82:3088-3101 PubMed35771632

2022

CHD1 Promotes Sensitivity to Aurora Kinase Inhibitors by Suppressing Interaction of AURKA with Its Coactivator TPX2.

Applications

Unspecified application

Species

Unspecified reactive species

Haoyan Li,Yin Wang,Kevin Lin,Varadha Balaji Venkadakrishnan,Martin Bakht,Wei Shi,Chenling Meng,Jie Zhang,Kaitlyn Tremble,Xin Liang,Jian H Song,Xu Feng,Vivien Van,Pingna Deng,Jared K Burks,Ana Aparicio,Khandan Keyomarsi,Junjie Chen,Yue Lu,Himisha Beltran,Di Zhao

Communications biology 5:483 PubMed35590097

2022

SARS-CoV-2 ORF6 disrupts nucleocytoplasmic trafficking to advance viral replication.

Applications

Unspecified application

Species

Unspecified reactive species

Yoichi Miyamoto,Yumi Itoh,Tatsuya Suzuki,Tomohisa Tanaka,Yusuke Sakai,Masaru Koido,Chiaki Hata,Cai-Xia Wang,Mayumi Otani,Kohji Moriishi,Taro Tachibana,Yoichiro Kamatani,Yoshihiro Yoneda,Toru Okamoto,Masahiro Oka

Bioengineered 13:7829-7846 PubMed35291921

2022

Long non-coding RNA HAGLROS facilitates tumorigenesis and progression in hepatocellular carcinoma by sponging miR-26b-5p to up-regulate karyopherin α2 (KPNA2) and inactivate p53 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Gaofeng Tang,Huibo Zhao,Zhantao Xie,Sidong Wei,Guoyong Chen

Journal of translational medicine 19:112 PubMed33731128

2021

KPNA2 interaction with CBX8 contributes to the development and progression of bladder cancer by mediating the PRDM1/c-FOS pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fanchang Zeng,Liumei Luo,Daoyuan Li,Juncheng Guo,Min Guo

Journal of immunology research 2021:6694392 PubMed33728352

2021

Increased Nuclear Transporter KPNA2 Contributes to Tumor Immune Evasion by Enhancing PD-L1 Expression in PDAC.

Applications

Unspecified application

Species

Unspecified reactive species

Kai-Xia Zhou,Shan Huang,Li-Peng Hu,Xue-Li Zhang,Wei-Ting Qin,Yan-Li Zhang,Lin-Li Yao,Yanqiu Yu,Yao-Qi Zhou,Lei Zhu,Jianguang Ji,Zhi-Gang Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KPNA2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA2 antibody (AB84440)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA2 antibody (AB84440)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA2 antibody (AB84440)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA2 antibody (AB84440)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA2 antibody (AB84440)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (32)

Product promise