AB170495

Anti-KPNA2 antibody [EPR11716(B)]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Recombinant Monoclonal KPNA2 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Rat samples. Cited in 3 publications.

View Alternative Names

RCH1, SRP1, KPNA2, Importin subunit alpha-1, Karyopherin subunit alpha-2, RAG cohort protein 1, SRP1-alpha

2 Images

Flow Cytometry (Intracellular) - Anti-KPNA2 antibody [EPR11716(B)] (AB170495)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-KPNA2 antibody [EPR11716(B)] (AB170495)

Intracellular flow cytometric analysis of permeabilized 293T cells labeling KPNA2 with ab170495 at 1/10 dilution (red) or a rabbit IgG negative control (green).

Supplier Data

Western blot - Anti-KPNA2 antibody [EPR11716(B)] (AB170495)

All lanes:

Western blot - Anti-KPNA2 antibody [EPR11716(B)] (ab170495) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Lane 5:

C6 cell lysate at 10 µg

Predicted band size: 57 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11716(B)

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KPNA2 also known as Importin subunit alpha-1 functions as a facilitator for nuclear protein import. It has a mass of about 58 kDa and resides mainly in the cytoplasm and nucleus. This protein binds with specific nuclear localization signals on cargo proteins and assists in their transport into the nucleus through the nuclear pore complex. KPNA2 expression levels vary between tissues but it often appears in higher amounts in proliferative tissues.

Biological function summary

KPNA2 plays an important role in cellular processes by forming a complex with importin beta aiding nuclear-cytoplasmic transport. This transport system is important for regulating gene expression and maintaining cellular homeostasis. KPNA2 influences the correct localization of many signaling and transcription factors impacting numerous cellular functions including cell cycle progression and DNA repair.

Pathways

KPNA2 is a critical player in the nuclear import pathway and intersects with the cell cycle regulation pathway. It is closely associated with proteins such as importin beta which together facilitate the translocation of proteins into the nucleus. KPNA2's function in importing various regulators ensures proper cell cycle progression and responds to cellular stress signals.

KPNA2 is linked to several cancers particularly breast and ovarian cancer. Its overexpression often correlates with poor prognosis in these cancers suggesting a role in tumor progression. KPNA2 interacts with oncogenic proteins like MYC and AURKA in these pathological states influencing tumor growth and survival mechanisms.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1 (PubMed : 28991411, PubMed : 32130408, PubMed : 7604027, PubMed : 7754385). Binds specifically and directly to substrates containing either a simple or bipartite NLS motif (PubMed : 28991411, PubMed : 32130408, PubMed : 7604027, PubMed : 7754385). Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed : 7604027, PubMed : 7754385). At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediator of PR-DUB complex component BAP1 nuclear import; acts redundantly with KPNA1 and Transportin-1/TNPO1 (PubMed : 35446349).

See full target information KPNA2

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Technology in cancer research & treatment 20:15330338211055948 PubMed34816790

2021

MiR-101-3p Suppresses Progression of Cervical Squamous Cell Carcinoma by Targeting and Down-Regulating KPNA2.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Wang,Rongxin Xiao,Biao Yang

Journal of ovarian research 14:71 PubMed34034774

2021

Increased expression of KPNA2 predicts unfavorable prognosis in ovarian cancer patients, possibly by targeting KIF4A signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangrong Cui,Honghong Wang,Xueqing Wu,Kai Huo,Xuan Jing

OncoTargets and therapy 12:6991-7004 PubMed31695414

2019

SNHG8 is upregulated in esophageal squamous cell carcinoma and directly sponges microRNA-411 to increase oncogenicity by upregulating KPNA2.

Applications

Unspecified application

Species

Unspecified reactive species

Huali Song,Jinxia Song,Lianwei Lu,Shoubo Li

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KPNA2 antibody [EPR11716(B)]

Flow Cytometry (Intracellular) - Anti-KPNA2 antibody [EPR11716(B)] (AB170495)

Western blot - Anti-KPNA2 antibody [EPR11716(B)] (AB170495)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise