JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB317093

Anti-KPNA3 antibody [HL1573]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal KPNA3 antibody. Suitable for IHC-P, WB and reacts with Rat, Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human KPNA3 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

QIP2, KPNA3, Importin subunit alpha-4, Importin alpha Q2, Karyopherin subunit alpha-3, SRP1-gamma, Qip2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA3 antibody [HL1573] (AB317093)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA3 antibody [HL1573] (AB317093)

ab317093 detects KPNA3 protein by immunohistochemical analysis.
Sample : Paraffin-embedded rat liver tissue.
KPNA3 stained by ab317093 diluted at 1 : 100.
Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Western blot - Anti-KPNA3 antibody [HL1573] (AB317093)
  • WB

Supplier Data

Western blot - Anti-KPNA3 antibody [HL1573] (AB317093)

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab317093 diluted at 1 : 1000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-KPNA3 antibody [HL1573] (ab317093) at 1/1000 dilution

Lane 1:

HeLa whole cell extract at 30 µg

Lane 2:

HepG2 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-KPNA3 antibody [HL1573] (AB317093)
  • WB

Supplier Data

Western blot - Anti-KPNA3 antibody [HL1573] (AB317093)

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab317093 diluted at 1 : 1000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-KPNA3 antibody [HL1573] (ab317093) at 1/1000 dilution

Lane 1:

Mouse muscle tissue extract at 50 µg

Lane 2:

Mouse spleen tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1573

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human KPNA3 conjugated to Keyhole Limpet Haemocyanin.

O00505

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KPNA3 also known as importin subunit alpha-4 is a protein that mediates the nuclear import of proteins by recognizing nuclear localization signals. Its molecular mass is approximately 58 kDa. KPNA3 is present in various tissues including brain liver and heart which suggests its importance in cellular functions across these organs.
Biological function summary

KPNA3 facilitates the transport of proteins into the nucleus serving as an adapter between cargo proteins and the importin beta subunit. KPNA3 is part of the importin complex which is important for nucleocytoplasmic transport. The efficiency of nuclear protein import depends on KPNA3's ability to bind and release cargo proteins effectively.

Pathways

KPNA3 integrates into the nucleocytoplasmic transport pathways and interacts significantly with importin beta. It participates in the regulation of gene expression by ensuring the proper localization of transcription factors. The protein is also connected to other members of the importin family such as KPNA1 and KPNA2 which similarly serve roles in nuclear import pathways.

KPNA3 has links to various conditions such as viral infections and cancer. The protein can interact with viral proteins to facilitate the transport of viral genomes into the nucleus. In cancer KPNA3 expression levels often correlate with the deregulation of nuclear import pathways influencing tumor progression. KPNA3's interactions with proteins like p53 highlight its relevance in understanding disease mechanisms.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Recognizes NLSs of influenza A virus nucleoprotein probably through ARM repeats 7-9.
See full target information KPNA3
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com