Anti-KPNA4 antibody [EPR26347-21] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA4 antibody [EPR26347-21] - BSA and Azide free (AB302557)

This data was developed using ab302556, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling KPNA4 with ab302556 at 1/5000 (0.109 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on human lung (PMID : 32320654).The section was incubated with ab302556 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA4 antibody [EPR26347-21] - BSA and Azide free (AB302557)

This data was developed using ab302556, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human colon carcinom tissue labeling KPNA4 with ab302556 at 1/5000 (0.109 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on human colon carcinoma (PMID : 31822798).The section was incubated with ab302556 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA4 antibody [EPR26347-21] - BSA and Azide free (AB302557)

This data was developed using ab302556, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling KPNA4 with ab302556 at 1/5000 (0.109 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on human testis (PMID : 9395085).The section was incubated with ab302556 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-KPNA4 antibody [EPR26347-21] - BSA and Azide free (AB302557)

This data was developed using ab302556, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : Human kidney (PMID : 9395085).

All lanes:

Western blot - Anti-KPNA4 antibody [EPR26347-21] (<a href='/en-us/products/primary-antibodies/kpna4-antibody-epr26347-21-ab302556'>ab302556</a>) at 1/1000 dilution

Lane 1:

Human testis tissue lysate 20 µg

Lane 2:

Human kidney tissue lysate 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/4000 dilution

Observed band size: 58 kDa

false

Exposure time: 59s

• WB

Supplier Data

Western blot - Anti-KPNA4 antibody [EPR26347-21] - BSA and Azide free (AB302557)

This data was developed using ab302556, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-KPNA4 antibody [EPR26347-21] (<a href='/en-us/products/primary-antibodies/kpna4-antibody-epr26347-21-ab302556'>ab302556</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) whole cell lysate 20 µg

Lane 2:

A549 (human lu carcinoma epithelial cell) whole cell lysate 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 20 µg

Lane 4:

L-929 (mouse connective tissue fibroblast) whole cell lysate 20 µg

Lane 5:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate 20 µg

Lane 7:

Raji (human burkitts lymphoma b lymphocyte) whole cell lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 58 kDa

false

• WB

Supplier Data

Western blot - Anti-KPNA4 antibody [EPR26347-21] - BSA and Azide free (AB302557)

This data was developed using ab302556, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-KPNA4 antibody [EPR26347-21] (<a href='/en-us/products/primary-antibodies/kpna4-antibody-epr26347-21-ab302556'>ab302556</a>) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate 20 µg

Lane 2:

Mouse ovary tissue lysate 20 µg

Lane 3:

Rat testis tissue lysate 20 µg

Lane 4:

Rat ovary tissue lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 58 kDa

false

Exposure time: 81s