Sample Prep & Detection Kits
Conjugation kitsPurification kitsSample preparation kitsChromogen kitsIHC kitsChIP kitsAccessory Reagents & Controls
Accessory reagents & controlsBiochemicals
BiochemicalsProteins and Peptides
Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
Learn about all product ranges with our product overviews.
Featured events
Make new connections at our global events.
Our programs
New Lab Program
Get a head start with our exclusive new lab discount. Enjoy 20% off and free shipping for three months.
New Biotech Program
Just starting out? Get 15% off and free shipping to your lab for six months.
Product promise
Peace of mind that all products perform as stated.
Product reviews
Leave reviews, get rewarded and help your community.
Trial program
Try untested species and applications to earn money off your next order.
Product Insider Program
Be the first to know about our latest product launches - and unlock exclusive offers and discounts.
Rabbit Polyclonal KPNA5 antibody. N-terminal. Suitable for ICC, WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human KPNA5.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 99% PBS
Liquid
Polyclonal
ICC | WB | IHC-P | ICC/IF | |
---|---|---|---|---|
Human | Expected | Expected | Tested | Expected |
Mouse | Tested | Tested | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 5 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 5.00000-20.00000 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates nuclear import of STAT1 homodimers and STAT1/STAT2 heterodimers by recognizing non-classical NLSs of STAT1 and STAT2 through ARM repeats 8-9. Recognizes influenza A virus nucleoprotein through ARM repeat 7-9 In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
Importin subunit alpha-6, Karyopherin subunit alpha-5, KPNA5
Rabbit Polyclonal KPNA5 antibody. N-terminal. Suitable for ICC, WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human KPNA5.
Importin subunit alpha-6, Karyopherin subunit alpha-5, KPNA5
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 99% PBS
Liquid
Polyclonal
Affinity purification Immunogen
ab189987 is predicted to not cross-react with other Importin alpha family members.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
KPNA5 is important in the transportation of proteins across the nuclear envelope a process that regulates many cellular functions such as cell division signal transduction and transcriptional control. KPNA5 as part of the importin alpha/beta complex regulates the import of proteins like transcription factors and tumor suppressors into the nucleus. The efficient functioning of this complex ensures proper cellular responses and maintenance of cellular homeostasis. By controlling the nuclear-cytoplasmic transport KPNA5 facilitates the precise localization of proteins necessary for normal cell function.
The protein KPNA5 also known as importin alpha 5 belongs to the karyopherin alpha family. Mechanically it plays an important role in the nuclear import of proteins by recognizing and binding to nuclear localization signals (NLS) on cargo proteins. Importin alpha 5 acts as an adaptor bridging cargo proteins with importin beta which facilitates transport through the nuclear pore complex. KPNA5 has a molecular mass of approximately 60 kDa and is expressed in a wide range of tissues including the brain heart and pancreas indicating its involvement in various cellular processes.
KPNA5 is involved in the nuclear transport pathway and is closely associated with the nucleocytoplasmic transport pathway. It interacts with proteins such as importin beta1 for translocation through the nuclear pore complex. This interaction is essential for processes like the regulation of gene expression and signal-dependent nuclear import which are critical for maintaining cellular activities and responses. The precise regulation of these pathways ensures that cells respond appropriately to different physiological signals and stresses.
KPNA5 shows a connection to certain cancers and neurodegenerative diseases. Its role in nuclear-cytoplasmic transport can influence oncogenesis as disruption in the import of cancer-related proteins could lead to tumor progression. Additionally abnormal KPNA5 function may associate with neurodegenerative conditions where impaired protein transport affects neuronal health. In such disorders KPNA5 often interacts with tumor suppressor proteins and other proteins that are important for neuronal function illustrating its potential as a target for therapeutic intervention.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
All lanes: Western blot - Anti-KPNA5 antibody - N-terminal (AB189987) at 1 µg/mL
All lanes: EL4 cell lysate
Predicted band size: 60 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain cortex tissue labeling KPNA5 with ab189987 at 5 μg/ml.
Immunocytochemical analysis of EL4 cells labeling KPNA5 with ab189987 at 5 μg/ml.
Immunofluorescent analysis of EL4 cells labeling KPNA5 with ab189987 at 20 μg/ml.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com