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Rabbit Polyclonal KPNA5 antibody. N-terminal. Suitable for ICC, WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human KPNA5.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 99% PBS

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human KPNA5. The exact immunogen used to generate this antibody is proprietary information. Database link O15131
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCWBIHC-PICC/IF
Human
Expected
Expected
Tested
Expected
Mouse
Tested
Tested
Expected
Tested

Tested
Tested

Species

Mouse

Dilution info

5 µg/mL

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1 µg/mL

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

5 µg/mL

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

5.00000-20.00000 µg/mL

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates nuclear import of STAT1 homodimers and STAT1/STAT2 heterodimers by recognizing non-classical NLSs of STAT1 and STAT2 through ARM repeats 8-9. Recognizes influenza A virus nucleoprotein through ARM repeat 7-9 In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.

Alternative names

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Rabbit Polyclonal KPNA5 antibody. N-terminal. Suitable for ICC, WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human KPNA5.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human KPNA5. The exact immunogen used to generate this antibody is proprietary information. Database link O15131
Purification technique

Affinity purification Immunogen

Specificity

ab189987 is predicted to not cross-react with other Importin alpha family members.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

KPNA5 is important in the transportation of proteins across the nuclear envelope a process that regulates many cellular functions such as cell division signal transduction and transcriptional control. KPNA5 as part of the importin alpha/beta complex regulates the import of proteins like transcription factors and tumor suppressors into the nucleus. The efficient functioning of this complex ensures proper cellular responses and maintenance of cellular homeostasis. By controlling the nuclear-cytoplasmic transport KPNA5 facilitates the precise localization of proteins necessary for normal cell function.

Activity summary

The protein KPNA5 also known as importin alpha 5 belongs to the karyopherin alpha family. Mechanically it plays an important role in the nuclear import of proteins by recognizing and binding to nuclear localization signals (NLS) on cargo proteins. Importin alpha 5 acts as an adaptor bridging cargo proteins with importin beta which facilitates transport through the nuclear pore complex. KPNA5 has a molecular mass of approximately 60 kDa and is expressed in a wide range of tissues including the brain heart and pancreas indicating its involvement in various cellular processes.

Pathways

KPNA5 is involved in the nuclear transport pathway and is closely associated with the nucleocytoplasmic transport pathway. It interacts with proteins such as importin beta1 for translocation through the nuclear pore complex. This interaction is essential for processes like the regulation of gene expression and signal-dependent nuclear import which are critical for maintaining cellular activities and responses. The precise regulation of these pathways ensures that cells respond appropriately to different physiological signals and stresses.

Associated diseases and disorders

KPNA5 shows a connection to certain cancers and neurodegenerative diseases. Its role in nuclear-cytoplasmic transport can influence oncogenesis as disruption in the import of cancer-related proteins could lead to tumor progression. Additionally abnormal KPNA5 function may associate with neurodegenerative conditions where impaired protein transport affects neuronal health. In such disorders KPNA5 often interacts with tumor suppressor proteins and other proteins that are important for neuronal function illustrating its potential as a target for therapeutic intervention.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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4 product images

  • Western blot - Anti-KPNA5 antibody - N-terminal (ab189987), expandable thumbnail

    Western blot - Anti-KPNA5 antibody - N-terminal (ab189987)

    All lanes: Western blot - Anti-KPNA5 antibody - N-terminal (AB189987) at 1 µg/mL

    All lanes: EL4 cell lysate

    Predicted band size: 60 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA5 antibody - N-terminal (ab189987), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA5 antibody - N-terminal (ab189987)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain cortex tissue labeling KPNA5 with ab189987 at 5 μg/ml.

  • Immunocytochemistry - Anti-KPNA5 antibody - N-terminal (ab189987), expandable thumbnail

    Immunocytochemistry - Anti-KPNA5 antibody - N-terminal (ab189987)

    Immunocytochemical analysis of EL4 cells labeling KPNA5 with ab189987 at 5 μg/ml.

  • Immunocytochemistry/ Immunofluorescence - Anti-KPNA5 antibody - N-terminal (ab189987), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-KPNA5 antibody - N-terminal (ab189987)

    Immunofluorescent analysis of EL4 cells labeling KPNA5 with ab189987 at 20 μg/ml.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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