AB189987

Anti-KPNA5 antibody - N-terminal

Name: Anti-KPNA5 antibody - N-terminal (ab189987) | Abcam
Brand: Abcam Limited
SKU: AB189987
Price: 630 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal KPNA5 antibody. N-terminal. Suitable for ICC, WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human KPNA5.

View Alternative Names

Importin subunit alpha-6, Karyopherin subunit alpha-5, KPNA5

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA5 antibody - N-terminal (AB189987)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA5 antibody - N-terminal (AB189987)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain cortex tissue labeling KPNA5 with ab189987 at 5 μg/ml.

Immunocytochemistry/ Immunofluorescence - Anti-KPNA5 antibody - N-terminal (AB189987)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KPNA5 antibody - N-terminal (AB189987)

Immunofluorescent analysis of EL4 cells labeling KPNA5 with ab189987 at 20 μg/ml.

Supplier Data

Immunocytochemistry - Anti-KPNA5 antibody - N-terminal (AB189987)

Immunocytochemical analysis of EL4 cells labeling KPNA5 with ab189987 at 5 μg/ml.

Supplier Data

Western blot - Anti-KPNA5 antibody - N-terminal (AB189987)

All lanes:

Western blot - Anti-KPNA5 antibody - N-terminal (ab189987) at 1 µg/mL

Lane 1:

EL4 cell lysate

Lane 2:

EL4 cell lysate with immunizing peptide

Predicted band size: 60 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC, WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human KPNA5. The exact immunogen used to generate this antibody is proprietary information.

O15131

Specificity

ab189987 is predicted to not cross-react with other Importin alpha family members.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "5 µg/mL", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5-20 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein KPNA5 also known as importin alpha 5 belongs to the karyopherin alpha family. Mechanically it plays an important role in the nuclear import of proteins by recognizing and binding to nuclear localization signals (NLS) on cargo proteins. Importin alpha 5 acts as an adaptor bridging cargo proteins with importin beta which facilitates transport through the nuclear pore complex. KPNA5 has a molecular mass of approximately 60 kDa and is expressed in a wide range of tissues including the brain heart and pancreas indicating its involvement in various cellular processes.

Biological function summary

KPNA5 is important in the transportation of proteins across the nuclear envelope a process that regulates many cellular functions such as cell division signal transduction and transcriptional control. KPNA5 as part of the importin alpha/beta complex regulates the import of proteins like transcription factors and tumor suppressors into the nucleus. The efficient functioning of this complex ensures proper cellular responses and maintenance of cellular homeostasis. By controlling the nuclear-cytoplasmic transport KPNA5 facilitates the precise localization of proteins necessary for normal cell function.

Pathways

KPNA5 is involved in the nuclear transport pathway and is closely associated with the nucleocytoplasmic transport pathway. It interacts with proteins such as importin beta1 for translocation through the nuclear pore complex. This interaction is essential for processes like the regulation of gene expression and signal-dependent nuclear import which are critical for maintaining cellular activities and responses. The precise regulation of these pathways ensures that cells respond appropriately to different physiological signals and stresses.

KPNA5 shows a connection to certain cancers and neurodegenerative diseases. Its role in nuclear-cytoplasmic transport can influence oncogenesis as disruption in the import of cancer-related proteins could lead to tumor progression. Additionally abnormal KPNA5 function may associate with neurodegenerative conditions where impaired protein transport affects neuronal health. In such disorders KPNA5 often interacts with tumor suppressor proteins and other proteins that are important for neuronal function illustrating its potential as a target for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates nuclear import of STAT1 homodimers and STAT1/STAT2 heterodimers by recognizing non-classical NLSs of STAT1 and STAT2 through ARM repeats 8-9. Recognizes influenza A virus nucleoprotein through ARM repeat 7-9 In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.

See full target information KPNA5

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurodegeneration 20:94 PubMed40877951

2025

MS4A6A/Ms4a6d deficiency disrupts neuroprotective microglia functions and promotes inflammation in Alzheimer's disease model.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Shan Jiao,Yi-Jun Ge,Liang-Yu Huang,Ying Liu,Bang-Sheng Wu,Piao-Piao Lian,Yi-Ning Hao,Shan-Shan Han,Yi-Ting Li,Kai-Min Wu,Chen-Yun Wu,Tian-Lin Cheng,Peng Yuan,Jin-Tai Yu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-KPNA5 antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA5 antibody - N-terminal (AB189987)

Immunocytochemistry/ Immunofluorescence - Anti-KPNA5 antibody - N-terminal (AB189987)

Immunocytochemistry - Anti-KPNA5 antibody - N-terminal (AB189987)

Western blot - Anti-KPNA5 antibody - N-terminal (AB189987)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise