Rabbit Polyclonal KPNA6 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human KPNA6.
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Publications
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- Cell death and differentiation 22:1775-842015Notch signaling in response to excitotoxicity induces neurodegeneration via erroneous cell cycle reentry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS Marathe et. al.PubMed 25822340
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human KPNA6. The exact immunogen used to generate this antibody is proprietary information. Database link O60684
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 20 µg/mL | Notes - |
Target data
Function
Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
Alternative names
IPOA7, KPNA6, Importin subunit alpha-7, Karyopherin subunit alpha-6
Recommended products
Rabbit Polyclonal KPNA6 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human KPNA6.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human KPNA6. The exact immunogen used to generate this antibody is proprietary information. Database link O60684
- Purification technique
- Affinity purification Immunogen
- Specificity
ab105350 is predicted to not crossreact with other Importin
alpha family members.- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KPNA6 also known as importin alpha 7 functions in the nuclear import of proteins. It acts by forming a complex with importin beta allowing proteins to pass through the nuclear pore complex. KPNA6 has a molecular mass of approximately 60 kDa. Expression of KPNA6 occurs in several tissues including liver brain and testes emphasizing its widespread role in various physiological processes.
- Biological function summary
KPNA6 facilitates the translocation of proteins containing a nuclear localization signal into the nucleus. It is part of the classical importin alpha/beta complex where it binds cargo proteins and importin beta recognizes the nuclear pore complex. This interaction is essential for the regulation of gene expression DNA replication and cell cycle progression. KPNA6 participates in coordinating these cellular processes by ensuring proteins enter the nucleus with timely precision.
- Pathways
Nuclear transport mechanisms involve KPNA6 prominently. It plays a significant role in the nucleocytoplasmic transport pathway also contributing to the regulation of the cell cycle. Proteins related to these pathways include importin beta which partners with KPNA6 for efficient nuclear protein import. These pathways maintain cellular homeostasis and enable response to cellular stress or changes.
- Associated diseases and disorders
KPNA6 links to cancer progression and certain viral infections. Its dysregulation can affect tumor suppressor proteins and oncogenes leading to abnormal cell growth and cancer. KPNA6's interaction with viral proteins like those from HIV highlights its importance in viral pathogenesis where the virus exploits the nuclear import mechanism for replication. Understanding KPNA6 interactions provides insights into therapeutic targets for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-KPNA6 antibody (ab105350)
All lanes: Western blot - Anti-KPNA6 antibody (ab105350) at 1 µg/mL
All lanes: 293 cell lysate at 15 µg
Predicted band size: 60 kDa
Immunocytochemistry/ Immunofluorescence - Anti-KPNA6 antibody (ab105350)
Immunofluorescence of KPNA6 in 293 cells with ab105350 at 20 ug/mL.
Immunocytochemistry/ Immunofluorescence - Anti-KPNA6 antibody (ab105350)
Immunofluorescence of KPNA6 in 293 cells using ab105350 at 20 ug/ml.
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