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Rabbit Recombinant Monoclonal KPNB1 antibody. Carrier free. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P, WB and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNB1 antibody [EPR26137-52] - BSA and Azide free (AB313371), expandable thumbnail
  • Western blot - Anti-KPNB1 antibody [EPR26137-52] - BSA and Azide free (AB313371), expandable thumbnail
  • Western blot - Anti-KPNB1 antibody [EPR26137-52] - BSA and Azide free (AB313371), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-KPNB1 antibody [EPR26137-52] - BSA and Azide free (AB313371), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-KPNB1 antibody [EPR26137-52] - BSA and Azide free (AB313371), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow Cyt (Intra)IHC-PWBIP
Human
Tested
Tested
Tested
Tested
Not recommended
Mouse
Tested
Tested
Tested
Tested
Not recommended
Rat
Expected
Expected
Tested
Tested
Not recommended

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor (PubMed:10228156, PubMed:11682607, PubMed:11891849, PubMed:19386897, PubMed:20818336, PubMed:24699649, PubMed:7615630, PubMed:9687515). Acting autonomously, serves itself as NLS receptor (PubMed:10228156, PubMed:11682607, PubMed:11891849, PubMed:19386897, PubMed:20818336, PubMed:24699649, PubMed:7615630, PubMed:9687515). Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed:10228156, PubMed:11682607, PubMed:11891849, PubMed:19386897, PubMed:20818336, PubMed:24699649, PubMed:7615630, PubMed:9687515). At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin (PubMed:10228156, PubMed:11682607, PubMed:11891849, PubMed:19386897, PubMed:20818336, PubMed:24699649, PubMed:7615630, PubMed:9687515). The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed:10228156, PubMed:11682607, PubMed:11891849, PubMed:19386897, PubMed:24699649, PubMed:7615630, PubMed:9687515). Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5 (PubMed:11682607, PubMed:9687515). In association with IPO7, mediates the nuclear import of H1 histone (PubMed:10228156). In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones (By similarity). Imports MRTFA, SNAI1 and PRKCI into the nucleus (PubMed:11891849, PubMed:19386897, PubMed:20818336, PubMed:24699649). (Microbial infection) In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.

Alternative names

NTF97, KPNB1, Importin subunit beta-1, Importin-90, Karyopherin subunit beta-1, Nuclear factor p97, Pore targeting complex 97 kDa subunit, PTAC97

Recommended products

Rabbit Recombinant Monoclonal KPNB1 antibody. Carrier free. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR26137-52
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

KPNB1 also known as importin beta plays hard role in nucleocytoplasmic transport. This large protein with a mass approximately 97 kDa shuttles proteins and ribonucleoproteins into the nucleus. KPNB1 expression occurs widely in many tissues reflecting its essential function in cell operations. As a solenoid protein KPNB1 structure allows flexibility needed for diverse interactions with cargo proteins and nuclear pore complexes.

Biological function summary

Importin beta facilitates the import of nuclear localization signal (NLS)-bearing proteins into the nucleus. KPNB1 forms a complex with importin alpha to mediate import processes. Importin alpha binds to the NLS-containing cargo while KPNB1 interacts with the nuclear pore complex. This system oversees important regulation of several cellular processes including signal transduction and cell cycle progression. The protein's role helps to organize cellular response to various stimuli maintaining cellular homeostasis.

Pathways

KPNB1 contributes to regulation in the nuclear import and export pathways. It tightly associates with Ran-GTPase a protein involved in the Ran pathway. This interaction supports directionality and specificity of energy-dependent nuclear transport. Additionally KPNB1 associates with proteins like NUP50 and NUP98 functioning as a gatekeeper at the nuclear pore complex by controlling cargo passage. Through these pathways KPNB1 balances cellular imports and exports critical for cell health and functionality.

Associated diseases and disorders

KPNB1 has links to cancer notably in tumorigenesis and cancer progression. Studies show aberrant KPNB1 expression affects the nuclear import machinery supporting cancer cell proliferation and survival. KPNB1 interacts with oncogenic proteins such as c-Myc influencing transcription regulation in tumor cells. Furthermore KPNB1 holds relevance in neurodegenerative diseases like Alzheimer's where impaired transport function contributes to pathological accumulation of proteins in neuronal cells.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNB1 antibody [EPR26137-52] - BSA and Azide free (ab313371), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNB1 antibody [EPR26137-52] - BSA and Azide free (ab313371)

    This data was developed using Anti-KPNB1 antibody [EPR26137-52] ab313370, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling KPNB1 with Anti-KPNB1 antibody [EPR26137-52] ab313370 at 1/10000 (0.053 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human testis.The section was incubated with Anti-KPNB1 antibody [EPR26137-52] ab313370 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-KPNB1 antibody [EPR26137-52] - BSA and Azide free (ab313371), expandable thumbnail

    Western blot - Anti-KPNB1 antibody [EPR26137-52] - BSA and Azide free (ab313371)

    This data was developed using Anti-KPNB1 antibody [EPR26137-52] ab313370, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Exposure time: 26 seconds

    All lanes: Western blot - Anti-KPNB1 antibody [EPR26137-52] (Anti-KPNB1 antibody [EPR26137-52] ab313370) at 1/1000 dilution

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 2: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

    Lane 3: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

    Lane 4: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 5: Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 6: SK-OV-3 (human ovarian cancer epithelial cell) whole cell lysate at 20 µg

    Lane 7: Human testis tissue lysate at 20 µg

    Lane 8: Mouse testis tissue lysate at 20 µg

    Lane 9: Rat testis tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 97 kDa

    Exposure time: 26s

  • Western blot - Anti-KPNB1 antibody [EPR26137-52] - BSA and Azide free (ab313371), expandable thumbnail

    Western blot - Anti-KPNB1 antibody [EPR26137-52] - BSA and Azide free (ab313371)

    This data was developed using Anti-KPNB1 antibody [EPR26137-52] ab313370, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Exposure time: 26 seconds

    All lanes: Western blot - Anti-KPNB1 antibody [EPR26137-52] (Anti-KPNB1 antibody [EPR26137-52] ab313370) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 10 µg

    Lane 2: HeLa transfected with siRNA specifically targeti KPNB1 whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 97 kDa

    Exposure time: 26s

  • Flow Cytometry (Intracellular) - Anti-KPNB1 antibody [EPR26137-52] - BSA and Azide free (ab313371), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-KPNB1 antibody [EPR26137-52] - BSA and Azide free (ab313371)

    This data was developed using Anti-KPNB1 antibody [EPR26137-52] ab313370, the same antibody clone in a different buffer formulation.
    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling KPNB1 with Anti-KPNB1 antibody [EPR26137-52] ab313370 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-KPNB1 antibody [EPR26137-52] - BSA and Azide free (ab313371), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-KPNB1 antibody [EPR26137-52] - BSA and Azide free (ab313371)

    This data was developed using Anti-KPNB1 antibody [EPR26137-52] ab313370, the same antibody clone in a different buffer formulation.
    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling KPNB1 with Anti-KPNB1 antibody [EPR26137-52] ab313370 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-KPNB1 antibody [EPR26137-52] - BSA and Azide free (ab313371), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-KPNB1 antibody [EPR26137-52] - BSA and Azide free (ab313371)

    This data was developed using Anti-KPNB1 antibody [EPR26137-52] ab313370, the same antibody clone in a different buffer formulation.
    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling KPNB1 with Anti-KPNB1 antibody [EPR26137-52] ab313370 at 1/50 (10.5 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic and nuclear staining in NIH/3T3 cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-KPNB1 antibody [EPR26137-52] - BSA and Azide free (ab313371), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-KPNB1 antibody [EPR26137-52] - BSA and Azide free (ab313371)

    This data was developed using Anti-KPNB1 antibody [EPR26137-52] ab313370, the same antibody clone in a different buffer formulation.
    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling KPNB1 with Anti-KPNB1 antibody [EPR26137-52] ab313370 at 1/50 (10.5 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic and nuclear staining in HeLa cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNB1 antibody [EPR26137-52] - BSA and Azide free (ab313371), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNB1 antibody [EPR26137-52] - BSA and Azide free (ab313371)

    This data was developed using Anti-KPNB1 antibody [EPR26137-52] ab313370, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling KPNB1 with Anti-KPNB1 antibody [EPR26137-52] ab313370 at 1/10000 (0.053 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat testis.The section was incubated with Anti-KPNB1 antibody [EPR26137-52] ab313370 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNB1 antibody [EPR26137-52] - BSA and Azide free (ab313371), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNB1 antibody [EPR26137-52] - BSA and Azide free (ab313371)

    This data was developed using Anti-KPNB1 antibody [EPR26137-52] ab313370, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling KPNB1 with Anti-KPNB1 antibody [EPR26137-52] ab313370 at 1/10000 (0.053 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse testis.The section was incubated with Anti-KPNB1 antibody [EPR26137-52] ab313370 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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