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AB236508

Anti-KPNB1 antibody [OTI6H3]

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(1 Publication)

Mouse Monoclonal KPNB1 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human KPNB1 aa 1-200.

View Alternative Names

NTF97, KPNB1, Importin subunit beta-1, Importin-90, Karyopherin subunit beta-1, Nuclear factor p97, Pore targeting complex 97 kDa subunit, PTAC97

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNB1 antibody [OTI6H3] (AB236508)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNB1 antibody [OTI6H3] (AB236508)

Paraffin-embedded human adult heart tissue stained for KPNB1 using ab236508 at 1/2000 dilution in immunohistochemical analysis.

Western blot - Anti-KPNB1 antibody [OTI6H3] (AB236508)
  • WB

Supplier Data

Western blot - Anti-KPNB1 antibody [OTI6H3] (AB236508)

All lanes:

Western blot - Anti-KPNB1 antibody [OTI6H3] (ab236508) at 1/500 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 35 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell extract at 35 µg

Lane 3:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract at 35 µg

Lane 4:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract at 35 µg

Predicted band size: 97 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI6H3

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human KPNB1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q14974

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p>Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from cell culture supernatant.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KPNB1 also known as importin beta plays hard role in nucleocytoplasmic transport. This large protein with a mass approximately 97 kDa shuttles proteins and ribonucleoproteins into the nucleus. KPNB1 expression occurs widely in many tissues reflecting its essential function in cell operations. As a solenoid protein KPNB1 structure allows flexibility needed for diverse interactions with cargo proteins and nuclear pore complexes.
Biological function summary

Importin beta facilitates the import of nuclear localization signal (NLS)-bearing proteins into the nucleus. KPNB1 forms a complex with importin alpha to mediate import processes. Importin alpha binds to the NLS-containing cargo while KPNB1 interacts with the nuclear pore complex. This system oversees important regulation of several cellular processes including signal transduction and cell cycle progression. The protein's role helps to organize cellular response to various stimuli maintaining cellular homeostasis.

Pathways

KPNB1 contributes to regulation in the nuclear import and export pathways. It tightly associates with Ran-GTPase a protein involved in the Ran pathway. This interaction supports directionality and specificity of energy-dependent nuclear transport. Additionally KPNB1 associates with proteins like NUP50 and NUP98 functioning as a gatekeeper at the nuclear pore complex by controlling cargo passage. Through these pathways KPNB1 balances cellular imports and exports critical for cell health and functionality.

KPNB1 has links to cancer notably in tumorigenesis and cancer progression. Studies show aberrant KPNB1 expression affects the nuclear import machinery supporting cancer cell proliferation and survival. KPNB1 interacts with oncogenic proteins such as c-Myc influencing transcription regulation in tumor cells. Furthermore KPNB1 holds relevance in neurodegenerative diseases like Alzheimer's where impaired transport function contributes to pathological accumulation of proteins in neuronal cells.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor (PubMed : 10228156, PubMed : 11682607, PubMed : 11891849, PubMed : 19386897, PubMed : 20818336, PubMed : 24699649, PubMed : 7615630, PubMed : 9687515). Acting autonomously, serves itself as NLS receptor (PubMed : 10228156, PubMed : 11682607, PubMed : 11891849, PubMed : 19386897, PubMed : 20818336, PubMed : 24699649, PubMed : 7615630, PubMed : 9687515). Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed : 10228156, PubMed : 11682607, PubMed : 11891849, PubMed : 19386897, PubMed : 20818336, PubMed : 24699649, PubMed : 7615630, PubMed : 9687515). At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin (PubMed : 10228156, PubMed : 11682607, PubMed : 11891849, PubMed : 19386897, PubMed : 20818336, PubMed : 24699649, PubMed : 7615630, PubMed : 9687515). The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed : 10228156, PubMed : 11682607, PubMed : 11891849, PubMed : 19386897, PubMed : 24699649, PubMed : 7615630, PubMed : 9687515). Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5 (PubMed : 11682607, PubMed : 9687515). In association with IPO7, mediates the nuclear import of H1 histone (PubMed : 10228156). In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones (By similarity). Imports MRTFA, SNAI1 and PRKCI into the nucleus (PubMed : 11891849, PubMed : 19386897, PubMed : 20818336, PubMed : 24699649).. (Microbial infection) In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.
See full target information KPNB1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Arthritis research & therapy 26:222 PubMed39702240

2024

KLF2 controls the apoptosis of neutrophils and is associated with disease activity of systemic lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Hongshuai Zhao,Zaichuan Lin,Peiwen Zhang,Jiayue Rao,Shumin Xu,Qing Luo,Junming Li
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Product promise

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