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AB122201

Anti-KPRP antibody

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(1 Publication)

Rabbit Polyclonal KPRP antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human KPRP.

View Alternative Names

C1orf45, KPRP, Keratinocyte proline-rich protein, hKPRP

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPRP antibody (AB122201)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPRP antibody (AB122201)

ab122201, at 1/200 dilution, staining KPRP in paraffin-embedded Human skin tissue by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-KPRP antibody (AB122201)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-KPRP antibody (AB122201)

Immunofluorescent staining of Human cell line U-251MG shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab122201 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human KPRP. The exact immunogen used to generate this antibody is proprietary information.

Q5T749

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Recommend PFA Fixation and Triton X-100 treatment</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle|Store undiluted
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KPRP also known as Keratinocyte Proline-Rich Protein or PGR9 is a structural protein involved in skin differentiation processes. This protein has a molecular weight of approximately 19 kDa. KPRP expression primarily occurs in the skin specifically in keratinocytes the predominant cell type in the epidermis. It plays a mechanical role in creating a protective barrier function of the skin by contributing to the formation of the cornified envelope the outermost layer of the skin.
Biological function summary

KPRP participates in skin homeostasis and barrier integrity. It interacts with other structural proteins to aid in the construction and maintenance of the cornified envelope. Although KPRP is not part of a known protein complex its role is significant in epidermal development and repair. It contributes to keratinocyte differentiation affecting the skin's ability to respond to external stressors.

Pathways

KPRP is relevant in skin differentiation and barrier function processes. It is part of pathways related to epidermal development influencing normal skin desquamation. KPRP does not have direct involvement with major pathways like Wnt or Notch but indirectly supports various cascades that ensure efficient skin cell turnover and maturation. KPRP interacts with other proteins such as keratins to establish a resilient skin surface.

KPRP has associations with skin-related conditions like psoriasis and dermatitis. Psoriasis causes changes in keratinocyte proliferation and differentiation where altered expression of KPRP may contribute to disease pathogenesis. Additionally in dermatitis KPRP plays a role in the skin's response to inflammation and environmental triggers. Its interaction with filaggrin another key skin protein provides insight into its involvement in maintaining barrier function and how disruptions can lead to or exacerbate these skin conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information KPRP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of investigative dermatology 139:1867-1875.e7 PubMed30905808

2019

Keratinocyte Proline-Rich Protein Deficiency in Atopic Dermatitis Leads to Barrier Disruption.

Applications

Unspecified application

Species

Unspecified reactive species

Hiraku Suga,Tomonori Oka,Makoto Sugaya,Yasunari Sato,Tsuyoshi Ishii,Hiroyuki Nishida,Shumpei Ishikawa,Masashi Fukayama,Shinichi Sato
View all publications
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Product promise

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