Rabbit Polyclonal KPRP antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human KPRP.
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Publications
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- The Journal of investigative dermatology 139:1867-1875.e72019Keratinocyte Proline-Rich Protein Deficiency in Atopic Dermatitis Leads to Barrier Disruption.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiraku Suga et. al.PubMed 30905808
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human KPRP. The exact immunogen used to generate this antibody is proprietary information. Database link Q5T749
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-4.00000 µg/mL | Notes Recommend PFA Fixation and Triton X-100 treatment |
Target data
Alternative names
C1orf45, KPRP, Keratinocyte proline-rich protein, hKPRP
Recommended products
Rabbit Polyclonal KPRP antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human KPRP.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human KPRP. The exact immunogen used to generate this antibody is proprietary information. Database link Q5T749
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle, Store undiluted
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KPRP also known as Keratinocyte Proline-Rich Protein or PGR9 is a structural protein involved in skin differentiation processes. This protein has a molecular weight of approximately 19 kDa. KPRP expression primarily occurs in the skin specifically in keratinocytes the predominant cell type in the epidermis. It plays a mechanical role in creating a protective barrier function of the skin by contributing to the formation of the cornified envelope the outermost layer of the skin.
- Biological function summary
KPRP participates in skin homeostasis and barrier integrity. It interacts with other structural proteins to aid in the construction and maintenance of the cornified envelope. Although KPRP is not part of a known protein complex its role is significant in epidermal development and repair. It contributes to keratinocyte differentiation affecting the skin's ability to respond to external stressors.
- Pathways
KPRP is relevant in skin differentiation and barrier function processes. It is part of pathways related to epidermal development influencing normal skin desquamation. KPRP does not have direct involvement with major pathways like Wnt or Notch but indirectly supports various cascades that ensure efficient skin cell turnover and maturation. KPRP interacts with other proteins such as keratins to establish a resilient skin surface.
- Associated diseases and disorders
KPRP has associations with skin-related conditions like psoriasis and dermatitis. Psoriasis causes changes in keratinocyte proliferation and differentiation where altered expression of KPRP may contribute to disease pathogenesis. Additionally in dermatitis KPRP plays a role in the skin's response to inflammation and environmental triggers. Its interaction with filaggrin another key skin protein provides insight into its involvement in maintaining barrier function and how disruptions can lead to or exacerbate these skin conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-KPRP antibody (ab122201)
Immunofluorescent staining of Human cell line U-251MG shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab122201 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPRP antibody (ab122201)
ab122201, at 1/200 dilution, staining KPRP in paraffin-embedded Human skin tissue by Immunohistochemistry.
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