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AB195334

Anti-KRAP antibody

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(3 Publications)

Rabbit Polyclonal KRAP antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ITPRID2 aa 1200-1300.

View Alternative Names

CS1, KIAA1927, KRAP, SSFA2, ITPRID2, Protein ITPRID2, Cleavage signal-1 protein, ITPR-interacting domain-containing protein 2, Ki-ras-induced actin-interacting protein, Sperm-specific antigen 2, CS-1

2 Images
Immunoprecipitation - Anti-KRAP antibody (AB195334)
  • IP

Supplier Data

Immunoprecipitation - Anti-KRAP antibody (AB195334)

Immunoprecipitation of 293T whole cell lysate in NETN lysis buffer using ab195334 (lane 1) at 6 μg/mg lysate (0.5 or 1 mg/IP; 20% of IP loaded/lane). Immunoblot analysis of KRAP utilized ab195334 at 1 μg/mL. The lysate in lane 2 is precipitated with an IgG control antibody. Detection utilized chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-KRAP antibody (ab195334)

Predicted band size: 138 kDa

false

Western blot - Anti-KRAP antibody (AB195334)
  • WB

Supplier Data

Western blot - Anti-KRAP antibody (AB195334)

Western blot analysis labeling KRAP with ab195334 at 0.1 ug/ml.

All lanes:

Western blot - Anti-KRAP antibody (ab195334) at 0.1 µg/mL

Lane 1:

HeLa cell lysate at 50 µg

Lane 2:

HEK-293T cell lysate at 50 µg

Lane 3:

Jurkat cell lysate at 50 µg

Predicted band size: 138 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ITPRID2 aa 1200-1300. The exact immunogen used to generate this antibody is proprietary information.

P28290

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Cat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Common marmoset": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Macaque monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab195334 was affinity purified using an epitope specific to KRAP immobilized on a solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KRAP also known as Ki-ras-induced actin-interacting protein is an important target in cell biology. This protein has a mass of approximately 128 kDa and is expressed in various tissues including the liver kidney and colon. It often interacts with cellular components to influence their structures and functions. In these tissues KRAP localizes to the cytoskeletal and membrane compartments playing a role in maintaining cellular integrity.
Biological function summary

KRAP contributes significantly to cellular function regulation by modulating GTPase activity and calcium ion signaling. It functions as part of a complex that includes proteins related to actin filament dynamics and signal transduction. Through these interactions KRAP influences processes such as cell shape and motility. This highlights its role in cytoskeletal organization and intracellular signaling pathways essential for normal cellular operations.

Pathways

KRAP has important involvement in the Rho family GTPase signaling and calcium signaling pathways. These pathways are integral to various cellular processes including cell migration division and differentiation. KRAP interacts closely with proteins like Rac1 and Cdc42 which are key players in cytoskeletal reorganization and signal relay. Such interactions underline its significance in facilitating cellular responses to external and internal cues.

KRAP's malfunction can relate to conditions like cancer and neurodegenerative diseases. Abnormal expression or regulation of KRAP affects cell structure and signaling leading to tumorigenesis or degeneration. KRAP also connects to proteins such as Ras and amyloid-beta in these diseases linking its structural and signaling roles to pathologies. Understanding its role could guide strategies for therapeutic intervention or disease management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information ITPRID2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Experimental & molecular medicine 53:1902-1910 PubMed34921223

2021

Epigenetic silencing of UBXN8 contributes to leukemogenesis in t(8;21) acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Erna Yang,Wei Guan,Desheng Gong,Jieying Li,Caixia Han,Juan Zhang,Hong Wang,Synat Kang,Xuefeng Gao,Yonghui Li,Li Yu

European journal of histochemistry : EJH 65: PubMed34538046

2021

miRNA-877-5p inhibits malignant progression of prostate cancer by directly targeting SSFA2.

Applications

Unspecified application

Species

Unspecified reactive species

Wanchun Wang,Jun Yi,Degang Dong,Wenli Mao,Xuanyu Wang,Zhangren Yan

Experimental and therapeutic medicine 21:549 PubMed33850521

2021

MicroRNA-363-3p inhibits tumor cell proliferation and invasion in oral squamous cell carcinoma cell lines by targeting SSFA2.

Applications

Unspecified application

Species

Unspecified reactive species

Liangming Zhu,Lei Zhang,Ying Tang,Fang Zhang,Chao Wan,Liang Xu,Ping Guo
View all publications
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Product promise

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