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AB275876

Anti-KRAS antibody [EPR23474-76]

  • 20ul selling size
  • KO Validated
  • RabMAb
  • Recombinant
  • What is this?

3

(8 Reviews)

|

(18 Publications)

Anti-KRAS antibody [EPR23474-76] (ab275876) is a rabbit monoclonal antibody detecting KRAS in Western Blot, IP. Suitable for Human.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

KRAS2, RASK2, KRAS, GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras

4 Images
Immunoprecipitation - Anti-KRAS antibody [EPR23474-76] (AB275876)
  • IP

Unknown

Immunoprecipitation - Anti-KRAS antibody [EPR23474-76] (AB275876)

KRAS was immunoprecipitated from 0.35 mg Human brain tissue lysate with ab275876 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275876 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Human brain tissue lysate 10 ug

Lane 2 : ab275876 IP in Human brain tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab275876 in human brain tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-KRAS antibody [EPR23474-76] (ab275876)

Predicted band size: 22 kDa

Observed band size: 21 kDa

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Western blot - Anti-KRAS antibody [EPR23474-76] (AB275876)
  • WB

Lab

Western blot - Anti-KRAS antibody [EPR23474-76] (AB275876)

Anti-KRAS antibody [EPR23474-76] (ab275876) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab275876 was shown to bind specifically to KRAS. A band was observed at 21 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in KRAS knockout cell line ab276083 (knockout cell lysate ab284205). To generate this image, wild-type and KRAS knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% BSA in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were VeriBlot for IP Detection Reagent (HRP) (ab131366) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-KRAS antibody [EPR23474-76] (ab275876) at 1/1000 dilution

Lane 1:

Wild-type HCT 116 cell lysate at 10 µg

Lane 2:

KRAS knockout HCT 116 cell lysate at 10 µg

Observed band size: 21 kDa

false

Western blot - Anti-KRAS antibody [EPR23474-76] (AB275876)
  • WB

Lab

Western blot - Anti-KRAS antibody [EPR23474-76] (AB275876)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes

All lanes:

Western blot - Anti-KRAS antibody [EPR23474-76] (ab275876) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 40 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 22 kDa

Observed band size: 21 kDa

false

Western blot - Anti-KRAS antibody [EPR23474-76] (AB275876)
  • WB

Lab

Western blot - Anti-KRAS antibody [EPR23474-76] (AB275876)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-KRAS antibody [EPR23474-76] (ab275876) at 1/1000 dilution

Lane 1:

His-tagged human KRAS recombinant protein, 10 ng

Lane 2:

His-tagged human HRAS recombinant protein (aa 1-186), 20 ng

Lane 3:

His-tagged human NRAS recombinant protein (aa 1-186), 20 ng

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 22 kDa

Observed band size: 21 kDa

false

  • 805 Alexa Fluor® 790

    Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 790) preadsorbed

  • Biotin

    Donkey Anti-Rabbit IgG H&L (Biotin)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23474-76

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>This antibody is not suitable for mouse or rat samples.</p>

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Reactivity data

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Product details

What is this antibody validated in?
Anti-KRAS antibody [EPR23474-76] (ab275876) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP) in Human samples.

What is the molecular weight of KRAS?
Anti-KRAS [EPR23474-76] (ab275876) specifically detects a band for KRAS (UniProt: P01116) at a molecular weight of 22kDa.

Reviewed by scientists
Anti-KRAS [EPR23474-76] (ab275876) has over 5 independent reviews from customers.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-KRAS antibody [EPR23474-76] (ab275876) has been confirmed by Western blot testing in KRAS Knockout HCT 116 cell line, ab276083.

Other related products
We have a range of other formats of antibody clone [EPR23474-76] also available for your convenience: ab275876, Carrier free - ab275885

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The KRAS protein also known as Kirsten rat sarcoma viral oncogene homolog is a small GTPase weighing around 21 kDa. It plays an important role in cell signaling pathways and is widely expressed in various tissues. As a molecular switch KRAS cycles between an active GTP-bound state and an inactive GDP-bound state regulating cell proliferation differentiation and survival. KRAS mutations are often studied using various cell lines including the HCT116 line which is a human colorectal carcinoma cell line with a KRAS mutant background. Western blot analysis commonly helps in investigating KRAS protein expression and activity levels.
Biological function summary

KRAS influences many cellular processes. It is not part of a complex but acts alone within its pathways. It modulates signal transduction processes which are significant for the control of proliferation apoptosis and cell cycle progress. Mutations within this protein such as KRAS G12V and KRAS G12D lead to continuous activation and can cause uncontrolled cell growth which links them to various cancers. KRAS commonly interacts with proteins like RAF kinase and PI3K signaling components to execute its functions.

Pathways

KRAS is an important component in at least two important signaling pathways: the MAPK/ERK pathway and the PI3K/AKT pathway. These pathways play major roles in regulating gene expression that drives cell growth and division. In the MAPK/ERK pathway KRAS activates RAF which subsequently initiates a phosphorylation cascade that stimulates ERK. This protein also interacts with elements of the PI3K/AKT pathway influencing cellular survival and metabolism. These interactions position KRAS as a vital oncogene in cancer biology.

KRAS mutations are important in the development and progression of colorectal and pancreatic cancers. The persistence of active KRAS leads to continuous signaling that drives tumor growth and resistance to apoptosis. KRAS-associated cancers often show poor prognosis partly due to limited therapeutic options. Its interaction with proteins like EGFR (Epidermal Growth Factor Receptor) in various cancers highlights complex treatment challenges as these proteins can provide alternative signaling routes further complicating disease management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed : 20949621, PubMed : 39809765). Plays an important role in the regulation of cell proliferation (PubMed : 22711838, PubMed : 23698361). Activates MAPK1/MAPK3 resulting in phosphorylation and ultimately degradation of GJA1 (By similarity). Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner (PubMed : 24623306).
See full target information KRAS
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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Advanced pharmaceutical bulletin 15:416-427 PubMed40922737

2025

Inhibitory Effects of Citrus-Derived Flavonoids Hesperidin and Hesperetin on SARS-CoV-2 Spike-Mediated Syncytia Formation Using In Vitro Cell Model.

Applications

Unspecified application

Species

Unspecified reactive species

Dennaya Kumara,Hayfa Salsabila Harsan,Endah Puji Septisetyani,Pekik Wiji Prasetyaningrum,Komang Alit Paramitasari,Mukh Syaifudin,Okid Parama Astirin,Muthi Ikawati,Edy Meiyanto

British journal of pharmacology 182:3923-3951 PubMed40344208

2025

A mitochondria-targeting and G-quadruplex structure-binding ligand inducing calcium overload and ferroptosis in human cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bo-Xin Zheng,Wei Long,Yao-Xun Zeng,Meng-Ting She,Yingying Zheng,Wen-De Zheng,Ya-Kun Wang,Ka-Hin Chan,Alan Siu-Lun Leung,Chun-Ming Chan,Yu-Jing Lu,Wing-Leung Wong

The Journal of clinical investigation 135: PubMed40091835

2025

Targeting ubiquitin-independent proteasome with small molecule increases susceptibility in pan-KRAS-mutant cancer models.

Applications

Unspecified application

Species

Unspecified reactive species

Shihui Shen,Qiansen Zhang,Yuhan Wang,Hui Chen,Shuangming Gong,Yun Liu,Conghao Gai,Hansen Chen,Enhao Zhu,Bo Yang,Lin Liu,Siyuan Cao,Mengting Zhao,Wenjie Ren,Mengjuan Li,Zhuoya Peng,Lu Zhang,Shaoying Zhang,Juwen Shen,Bianhong Zhang,Patrick Kh Lee,Kun Li,Lei Li,Huaiyu Yang

BMC pregnancy and childbirth 25:21 PubMed39789492

2025

High taurocholic acid concentration induces ferroptosis by downregulating FTH1 expression in intrahepatic cholestasis of pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Jian Zeng,Hua-Jing Yang,Ying-Jie Gu,Meng-Nan Yang,Meng-Ru Sun,Sheng-Kai Cheng,Yan-Yan Hou,Wei Gu

Science advances 10:eadp0684 PubMed39413197

2024

Semaphorin 3D promotes pancreatic ductal adenocarcinoma progression and metastasis through macrophage reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Noelle R J Thielman,Vanessa Funes,Sanjana Davuluri,Hector E Ibanez,Wei-Chih Sun,Juan Fu,Keyu Li,Stephen Muth,Xingyi Pan,Kenji Fujiwara, Dwayne L Thomas Ii,MacKenzie Henderson,Selina Shiqing Teh,Qingfeng Zhu,Elizabeth Thompson,Elizabeth M Jaffee,Alex Kolodkin,Fengxi Meng,Lei Zheng

Military Medical Research 10:68 PubMed38124228

2023

Elevated FBXL6 activates both wild-type KRAS and mutant KRAS and drives HCC tumorigenesis via the ERK/mTOR/PRELID2/ROS axis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hao-Jun Xiong,Hong-Qiang Yu,Jie Zhang,Lei Fang,Di Wu,Xiao-Tong Lin,Chuan-Ming Xie

Epigenetics 18:2254976 PubMed37691391

2023

DNMT1 expression partially dictates 5-Azacytidine sensitivity and correlates with RAS/MEK/ERK activity in gastric cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhangqian Chen,Lin Zhang,Yang Yang,Haiming Liu,Xiaoyu Kang,Yongzhan Nie,Daiming Fan

World journal of gastroenterology 29:4542-4556 PubMed37621755

2023

MiR-204-3p overexpression inhibits gastric carcinoma cell proliferation by inhibiting the MAPK pathway and RIP1/MLK1 necroptosis pathway to promote apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Li,Joanna J Tibenda,Yi Nan,Shi-Cong Huang,Na Ning,Guo-Qing Chen,Yu-Hua Du,Ya-Ting Yang,Fan-Di Meng,Ling Yuan

Journal of cellular and molecular medicine 27:1637-1652 PubMed37226642

2023

KRAS mutation-induced TOPK overexpression contributes to tumour progression in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Cai,Shuo Yao,Yanmei Zou,Hui Lu,Xiuqiong Chen,Yali Wang,Kun Zheng,Feng Zhu,Yihua Wang,Hua Xiong,Junfei Zhu

Aging 15:3839-3856 PubMed37171392

2023

Exploring the molecular mechanism of glycyrrhetinic acid in the treatment of gastric cancer based on network pharmacology and experimental validation.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Li,Yuhua Du,Shicong Huang,Yi Yang,Doudou Lu,Junfei Zhang,Yan Chen,Lei Zhang,Yi Nan,Ling Yuan
View all publications
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