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AB326684

Anti-KRAS + HRAS + NRAS antibody [SR1329]

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Rabbit Recombinant Monoclonal RASN antibody. Suitable for WB, ICC/IF and reacts with Human, Rat samples.

View Alternative Names

HRAS1, NRAS, GTPase NRas, Transforming protein N-Ras

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-KRAS + HRAS + NRAS antibody [SR1329] (AB326684)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KRAS + HRAS + NRAS antibody [SR1329] (AB326684)

Immunofluorescent analysis of HeLa cells, using KRAS + HRAS + NRAS Antibody. 4% polyformaldehyde fixed. 0.1% tritonX-100 permeable. DAPI stains the nucleus.

Western blot - Anti-KRAS + HRAS + NRAS antibody [SR1329] (AB326684)
  • WB

Supplier Data

Western blot - Anti-KRAS + HRAS + NRAS antibody [SR1329] (AB326684)

All lanes : Primary antibody at 1/1000 dilution

Lane1 : 293T cell lysate.Lane2 : C6 cell lysate. (Proteins at 20ug per lane)Secondary : Goat Anti-Rabbit IgG(HRP) at 1/20000 dilution. Predicted band size : 21kDa. Observed band size : 21kDa

All lanes:

Western blot - Anti-KRAS + HRAS + NRAS antibody [SR1329] (ab326684) at 1/1000 dilution

Lane 1:

293T cell lysate at 20 µg

Lane 2:

C6 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG(HRP) at 1/20000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SR1329

Isotype

IgG

Carrier free

No

Reacts with

Human, Rat

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
See full target information NRAS

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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