Rabbit Polyclonal KREMEN1 antibody. C-terminal. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human KREMEN1.
Images
Publications
Filter by
- Reproduction & fertility :2023Presence of KREMEN receptors for DKK1 in the preimplantation bovine embryo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThiago Fernandes Amaral,Yao Xiao,Surawich Jeensuk,Tatiane Silva Maia,Camila J Cuellar,Chloe A Gingerich,Tracy L Scheffler,Peter J HansenPubMed 37582174
- Journal of neuroinflammation 19:1292022DKK3 ameliorates neuropathic pain via inhibiting ASK-1/JNK/p-38-mediated microglia polarization and neuroinflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLong-Qing Zhang,Shao-Jie Gao,Jia Sun,Dan-Yang Li,Jia-Yi Wu,Fan-He Song,Dai-Qiang Liu,Ya-Qun Zhou,Wei MeiPubMed 35658977
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human KREMEN1. The exact immunogen used to generate this antibody is proprietary information. Database link Q96MU8
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Predicted | Predicted |
| Rat | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.50000-1.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Receptor for Dickkopf proteins. Cooperates with DKK1/2 to inhibit Wnt/beta-catenin signaling by promoting the endocytosis of Wnt receptors LRP5 and LRP6. In the absence of DKK1, potentiates Wnt-beta-catenin signaling by maintaining LRP5 or LRP6 at the cell membrane. Can trigger apoptosis in a Wnt-independent manner and this apoptotic activity is inhibited upon binding of the ligand DKK1. Plays a role in limb development; attenuates Wnt signaling in the developing limb to allow normal limb patterning and can also negatively regulate bone formation. Modulates cell fate decisions in the developing cochlea with an inhibitory role in hair cell fate specification.
Alternative names
KREMEN, KRM1, KREMEN1, Kremen protein 1, Dickkopf receptor, Kringle domain-containing transmembrane protein 1, Kringle-containing protein marking the eye and the nose
Recommended products
Rabbit Polyclonal KREMEN1 antibody. C-terminal. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human KREMEN1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human KREMEN1. The exact immunogen used to generate this antibody is proprietary information. Database link Q96MU8
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KREMEN1 also known as Kringle Containing Transmembrane Protein 1 is a transmembrane protein with a mass of approximately 56 kDa. It acts as a receptor involved in the regulation of the Wnt-signaling pathway. This protein participates in modulating cellular functions and development. KREMEN1 expression occurs in various tissues with significant presence noted in the brain kidney and lung. Its role as an antagonist in the pathway marks its importance in maintaining cellular homeostasis.
- Biological function summary
KREMEN1 interacts critically with other components of the Wnt pathway such as Dickkopf-1 (DKK1) to mediate cellular signaling. These interactions form a receptor complex with Lipoprotein receptor-related protein 6 (LRP6) which plays a significant role in inhibiting Wnt/beta-catenin signaling. This inhibition is important for processes such as cell differentiation and proliferation. KREMEN1's involvement in these processes highlights its contribution to controlling cellular behavior.
- Pathways
KREMEN1 participates primarily in the Wnt signaling pathway. It regulates this pathway by affecting the LRP6 co-receptor in conjunction with DKK1. Another significant pathway involving KREMEN1 is the bone morphogenetic protein (BMP) signaling pathway. Here its interactions illustrate a complex interplay with proteins like noggin and chordin contributing to embryo development and bone formation processes.
- Associated diseases and disorders
Alterations in KREMEN1 expression or function can relate to cancer and bone disorders. In cancers aberrant Wnt signaling often facilitated through dysregulation of KREMEN1 and DKK1 interaction contributes to tumor development and progression. Bone developmental disorders such as osteoporosis may arise from disruptions in KREMEN1's role in bone morphogenetic protein pathways. Understanding KREMEN1's connection with these conditions proves important in therapeutic development and research.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KREMEN1 antibody - C-terminal (ab211285)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast tissue labeling KREMEN1 with ab211285 at 5 μg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KREMEN1 antibody - C-terminal (ab211285)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labeling KREMEN1 with ab211285 at 5 μg/ml.
Western blot - Anti-KREMEN1 antibody - C-terminal (ab211285)
Lane 1: Western blot - Anti-KREMEN1 antibody - C-terminal (ab211285) at 0.5 µg/mL
Lane 2: Western blot - Anti-KREMEN1 antibody - C-terminal (ab211285) at 1 µg/mL
All lanes: Rat small intestine tissue lysate
Predicted band size: 52 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com