AB211285

Anti-KREMEN1 antibody - C-terminal

Name: Anti-KREMEN1 antibody - C-terminal (ab211285) | Abcam
Brand: Abcam Limited
SKU: AB211285
Price: 585 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(3 Publications)

Rabbit Polyclonal KREMEN1 antibody. C-terminal. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human KREMEN1.

View Alternative Names

KREMEN, KRM1, KREMEN1, Kremen protein 1, Dickkopf receptor, Kringle domain-containing transmembrane protein 1, Kringle-containing protein marking the eye and the nose

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KREMEN1 antibody - C-terminal (AB211285)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KREMEN1 antibody - C-terminal (AB211285)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast tissue labeling KREMEN1 with ab211285 at 5 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KREMEN1 antibody - C-terminal (AB211285)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labeling KREMEN1 with ab211285 at 5 μg/ml.

Supplier Data

Western blot - Anti-KREMEN1 antibody - C-terminal (AB211285)

Lane 1:

Western blot - Anti-KREMEN1 antibody - C-terminal (ab211285) at 0.5 µg/mL

Lane 2:

Western blot - Anti-KREMEN1 antibody - C-terminal (ab211285) at 1 µg/mL

All lanes:

Rat small intestine tissue lysate

Predicted band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human KREMEN1. The exact immunogen used to generate this antibody is proprietary information.

Q96MU8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KREMEN1 also known as Kringle Containing Transmembrane Protein 1 is a transmembrane protein with a mass of approximately 56 kDa. It acts as a receptor involved in the regulation of the Wnt-signaling pathway. This protein participates in modulating cellular functions and development. KREMEN1 expression occurs in various tissues with significant presence noted in the brain kidney and lung. Its role as an antagonist in the pathway marks its importance in maintaining cellular homeostasis.

Biological function summary

KREMEN1 interacts critically with other components of the Wnt pathway such as Dickkopf-1 (DKK1) to mediate cellular signaling. These interactions form a receptor complex with Lipoprotein receptor-related protein 6 (LRP6) which plays a significant role in inhibiting Wnt/beta-catenin signaling. This inhibition is important for processes such as cell differentiation and proliferation. KREMEN1's involvement in these processes highlights its contribution to controlling cellular behavior.

Pathways

KREMEN1 participates primarily in the Wnt signaling pathway. It regulates this pathway by affecting the LRP6 co-receptor in conjunction with DKK1. Another significant pathway involving KREMEN1 is the bone morphogenetic protein (BMP) signaling pathway. Here its interactions illustrate a complex interplay with proteins like noggin and chordin contributing to embryo development and bone formation processes.

Alterations in KREMEN1 expression or function can relate to cancer and bone disorders. In cancers aberrant Wnt signaling often facilitated through dysregulation of KREMEN1 and DKK1 interaction contributes to tumor development and progression. Bone developmental disorders such as osteoporosis may arise from disruptions in KREMEN1's role in bone morphogenetic protein pathways. Understanding KREMEN1's connection with these conditions proves important in therapeutic development and research.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for Dickkopf proteins. Cooperates with DKK1/2 to inhibit Wnt/beta-catenin signaling by promoting the endocytosis of Wnt receptors LRP5 and LRP6. In the absence of DKK1, potentiates Wnt-beta-catenin signaling by maintaining LRP5 or LRP6 at the cell membrane. Can trigger apoptosis in a Wnt-independent manner and this apoptotic activity is inhibited upon binding of the ligand DKK1. Plays a role in limb development; attenuates Wnt signaling in the developing limb to allow normal limb patterning and can also negatively regulate bone formation. Modulates cell fate decisions in the developing cochlea with an inhibitory role in hair cell fate specification.

See full target information KREMEN1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:110604 PubMed39252971

2024

Patient-derived tumor organoids mimic treatment-induced DNA damage response in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bernarda Majc,Anamarija Habič,Marta Malavolta,Miloš Vittori,Andrej Porčnik,Roman Bošnjak,Jernej Mlakar,Alenka Matjašič,Andrej Zupan,Marija Skoblar Vidmar,Tamara Lah Turnšek,Aleksander Sadikov,Barbara Breznik,Metka Novak

Reproduction & fertility : PubMed37582174

2023

Presence of KREMEN receptors for DKK1 in the preimplantation bovine embryo.

Applications

Unspecified application

Species

Unspecified reactive species

Thiago Fernandes Amaral,Yao Xiao,Surawich Jeensuk,Tatiane Silva Maia,Camila J Cuellar,Chloe A Gingerich,Tracy L Scheffler,Peter J Hansen

Journal of neuroinflammation 19:129 PubMed35658977

2022

DKK3 ameliorates neuropathic pain via inhibiting ASK-1/JNK/p-38-mediated microglia polarization and neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Long-Qing Zhang,Shao-Jie Gao,Jia Sun,Dan-Yang Li,Jia-Yi Wu,Fan-He Song,Dai-Qiang Liu,Ya-Qun Zhou,Wei Mei

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KREMEN1 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KREMEN1 antibody - C-terminal (AB211285)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KREMEN1 antibody - C-terminal (AB211285)

Western blot - Anti-KREMEN1 antibody - C-terminal (AB211285)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise