JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB235356

Anti-KRT35 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal KRT35 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human KRT35.

View Alternative Names

HHA5, HKA5, KRTHA5, KRT35, Keratin-35, K35

3 Images
Western blot - Anti-KRT35 antibody (AB235356)
  • WB

Supplier Data

Western blot - Anti-KRT35 antibody (AB235356)

All lanes:

Western blot - Anti-KRT35 antibody (ab235356) at 1/500 dilution

All lanes:

PC-3 (human prostate adenocarcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 50 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT35 antibody (AB235356)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT35 antibody (AB235356)

Paraffin-embedded human prostate cancer tissue stained for KRT35 using ab235356 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT35 antibody (AB235356)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT35 antibody (AB235356)

Paraffin-embedded human skin tissue stained for KRT35 using ab235356 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human KRT35.

Q92764

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KRT35 also known as Keratin 35 is a type I keratin protein. As a structural protein with a mass of around 44 kDa KRT35 is primarily found in the hair shaft cortex where it aids in maintaining the integrity of hair. The protein belongs to the broader keratin family which includes many other keratins classified by acidic or basic nature. KRT35 is expressed mainly in hair follicle cells and relates to the cytoskeleton serving as an important component that supports cellular structure.
Biological function summary

KRT35 contributes to the mechanical stability and resilience of hair by forming intermediate filaments which are part of the hair's structural matrix. The protein interacts within a complex network of type I and type II keratins enabling a robust structural framework necessary for healthy hair development. KRT35's role in hair morphology is especially significant during hair follicle formation and cycling contributing to the keratinization process that forms the hair shaft.

Pathways

KRT35 operates within the keratinization and epithelial cell differentiation pathways. It closely interacts with other type I and II keratin proteins like KRT85 and KRT86 creating a network that influences hair and epidermal tissue formation. Understanding these interactions helps interpret how KRT35 is involved in complex biological systems and how it maintains the structure of epithelial cells.

Malfunction or mutations of KRT35 associate with hair shaft disorders such as monilethrix an autosomal dominant condition causing fragile hair. It shows connection with other keratin proteins like KRT86 where mutations can similarly lead to defects in hair structure. Additionally changes in KRT35 expression can affect hair growth and quality emphasizing its importance in maintaining healthy hair phenotype.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information KRT35
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical endocrinology and metabolism 107:3328-3340 PubMed36074910

2022

Premature Ovarian Insufficiency in CLPB Deficiency: Transcriptomic, Proteomic and Phenotypic Insights.

Applications

Unspecified application

Species

Unspecified reactive species

Elena J Tucker,Megan J Baker,Daniella H Hock,Julia T Warren,Sylvie Jaillard,Katrina M Bell,Rajini Sreenivasan,Shabnam Bakhshalizadeh,Chloe A Hanna,Nikeisha J Caruana,Saskia B Wortmann,Shamima Rahman,Robert D S Pitceathly,Jean Donadieu,Aurelia Alimi,Vincent Launay,Paul Coppo,Sophie Christin-Maitre,Gorjana Robevska,Jocelyn van den Bergen,Brianna L Kline,Katie L Ayers,Phoebe N Stewart,David A Stroud,Diana Stojanovski,Andrew H Sinclair
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB109287

Anti-KAP1 antibody [EPR5216]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody [EPR5216] (AB109287)

  • 5
  • 4
Primary Antibodies

AB7800

Anti-Cytokeratin 14 antibody [LL002]

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [LL002] (AB7800)

  • 5
  • 27
Primary Antibodies

AB109545

Anti-KAP1 antibody [EPR5249]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-KAP1 antibody [EPR5249] (AB109545)

  • 2
  • 1
Primary Antibodies

AB76318

Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker

RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (AB76318)

  • 5
  • 14

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com