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AB154313

Anti-KRT6B antibody

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(4 Publications)

Rabbit Polyclonal K2C6B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KRT6B aa 100 to C-terminus.

View Alternative Names

K6B, KRTL1, KRT6B, Cytokeratin-6B, Keratin-6B, Type-II keratin Kb10, CK-6B

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-KRT6B antibody (AB154313)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-KRT6B antibody (AB154313)

Immunofluorescence analysis of methanol-fixed HeLa cells, labeling KRT6B using ab154313 at 1/500 dilution. Right panel costained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6B antibody (AB154313)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6B antibody (AB154313)

Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling KRT6B using ab154313 at 1/500 dilution.

Western blot - Anti-KRT6B antibody (AB154313)
  • WB

Unknown

Western blot - Anti-KRT6B antibody (AB154313)

7.5% SDS PAGE

All lanes:

Western blot - Anti-KRT6B antibody (ab154313) at 1/5000 dilution

All lanes:

HeLa cell lysate at 1 µg

Predicted band size: 60 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human KRT6B aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P04259

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KRT6B also known as Keratin 6B or CK6B is a type II cytoskeletal keratin. This protein has a molecular mass of approximately 60 kDa. It is mostly found in epithelial cells particularly in stratified squamous epithelia such as the epidermis hair follicles and oral mucosa. KRT6B participates in forming a structural network inside cells providing resilience to mechanical stress and contributing to the integrity and function of epithelial layers.
Biological function summary

KRT6B engages in cellular roles across various tissues. It is involved in the keratinocyte activation cycle and plays a part in wound healing processes. KRT6B forms a heterodimer complex with type I keratins like KRT16 and KRT17 stabilizing intermediate filaments that maintain cell structure. This assembly assists in skin health and influences cellular behaviors such as migration and differentiation especially during tissue repair.

Pathways

KRT6B integrates into signaling pathways like the Notch and Wnt pathways which regulate differentiation and proliferation in epithelial tissues. Within these pathways KRT6B interacts with proteins such as Notch receptors and components of the Wnt signaling cascade. Its association with these pathways highlights its role in cellular fate decisions and epidermal homeostasis reflecting its relevance to both normal and pathophysiological processes.

KRT6B links to conditions like pachyonychia congenita and psoriasis. Mutations in KRT6B can cause pachyonychia congenita where it affects nail and skin integrity. Additionally in psoriasis abnormal KRT6B expression relates to the rapid turnover of keratinocytes. Its interaction with keratin pairs like KRT16 and KRT17 during these disorders highlights its role in disease mechanisms and suggests potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information KRT6B
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 28:402 PubMed39234585

2024

PIN1‑silencing mitigates keratinocyte proliferation and the inflammatory response in psoriasis by activating mitochondrial autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Xia,Jin Li,Hongshan Yuan,Wenliang Yan

Genome medicine 16:20 PubMed38297291

2024

Integrative analysis of spatial and single-cell transcriptome data from human pancreatic cancer reveals an intermediate cancer cell population associated with poor prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Seongryong Kim,Galam Leem,Junjeong Choi,Yongjun Koh,Suho Lee,Sang-Hee Nam,Jin Su Kim,Chan Hee Park,Ho Kyoung Hwang,Kyoung Il Min,Jung Hyun Jo,Hee Seung Lee,Moon Jae Chung,Jeong Youp Park,Seung Woo Park,Si Young Song,Eui-Cheol Shin,Chang Moo Kang,Seungmin Bang,Jong-Eun Park

Andrology 9:657-664 PubMed33290605

2020

Testicular steroid sulfatase overexpression is associated with Leydig cell dysfunction in primary spermatogenic failure.

Applications

Unspecified application

Species

Unspecified reactive species

Maria C Lardone,Ian N Reyes,Eliana Ortiz,Antonio Piottante,Cristián Palma,Mauricio Ebensperger,Andrea Castro

Biochemical pharmacology 178:114048 PubMed32446889

2020

Novel epoxy-tiglianes stimulate skin keratinocyte wound healing responses and re-epithelialization via protein kinase C activation.

Applications

Unspecified application

Species

Unspecified reactive species

Rachael L Moses,Glen M Boyle,Rachel A Howard-Jones,Rachel J Errington,Jenny P Johns,Victoria Gordon,Paul Reddell,Robert Steadman,Ryan Moseley
View all publications
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