AB227643

Anti-KRT6C antibody [SP87]

20ul selling size
BOND RX™ Validated
RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal K2C6C antibody. Suitable for Flow Cyt (Intra), WB, IHC-P, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

KRT6E, KRT6C, Cytokeratin-6C, Cytokeratin-6E, Keratin K6h, Keratin-6C, Type-II keratin Kb12, CK-6C, CK-6E, K6C

20 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

Formalin-fixed paraffin-embedded human bladder tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

Formalin-fixed paraffin-embedded human stomach tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

Formalin-fixed paraffin-embedded human rectal adenocarcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

Formalin-fixed paraffin-embedded human ovarian adenocarcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-KRT6C antibody [SP87] (AB227643)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-KRT6C antibody [SP87] (AB227643)

Flow cytometry analysis of HaCaT (human skin keratinocyte) labeling KRT6C with purified ab227643 at 1/200 dilution (0.48 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

Formalin-fixed paraffin-embedded human placenta tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

Formalin-fixed paraffin-embedded human colon adenocarcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-KRT6C antibody [SP87] (AB227643)

Flow cytometric analysis of A431 (human epidermoid carcinoma cell line) cell line labeling KRT6C with ab227643 at 1/100 dilution (green) compared with a negative control rabbit IgG (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

Formalin-fixed paraffin-embedded human bladder transitional cell carcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-KRT6C antibody [SP87] (AB227643)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-KRT6C antibody [SP87] (AB227643)

Immunocytochemistry/ Immunofluorescence analysis of HaCaT (human skin keratinocyte) cells labeling KRT6C with purified ab227643 at 1/50 (1.92 μg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

Formalin-fixed paraffin-embedded human prostate tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

Formalin-fixed paraffin-embedded human lung squamous cell carcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

Formalin-fixed paraffin-embedded human breast tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate carcinoma tissue sections labeling KRT6C with ab227643 at 1/200 dilution (0.12 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Leica DS9800 (Bond™ Polymer Refine Detection) was used as a secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human prostate carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227643 for 10 mins at room temperature.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

Formalin-fixed paraffin-embedded human prostate carcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat skin tissue sections labeling KRT6C with ab227643 at 1/200 dilution (0.12 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Leica DS9800 (Bond™ Polymer Refine Detection) was used as a secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the rat skin, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227643 for 10 mins at room temperature.

Immunohistochemistry (Frozen sections) - Anti-KRT6C antibody [SP87] (AB227643)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-KRT6C antibody [SP87] (AB227643)

Immunohistochemistry (Frozen) analysis of rat skin tissue section labeling KRT6C with purified ab227643 at 1/25 (3.84μg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-KRT6C antibody [SP87] (AB227643)

Immunohistochemistry (Frozen) analysis of mouse skin tissue section labeling KRT6C with purified ab227643 at 1/25 (3.84μg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse skin tissue sections labeling KRT6C with ab227643 at 1/200 dilution (0.12 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Leica DS9800 (Bond™ Polymer Refine Detection) was used as a secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the mouse skin, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227643 for 10 mins at room temperature.

Supplier Data

Western blot - Anti-KRT6C antibody [SP87] (AB227643)

All lanes:

Western blot - Anti-KRT6C antibody [SP87] (ab227643) at 1/25 dilution

All lanes:

A431 (human epidermoid carcinoma cell line) whole cell lysate

Predicted band size: 60 kDa

true

Carrier free

Anti-KRT6C antibody [SP87] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP87

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, Flow Cyt (Intra), ICC/IF, IHC-Fr, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/200", "FlowCytIntra-species-notes": "Incubate with ab227643 for 30 minutes at 4°C.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/25", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "Boil tissue section in 10mM citrate, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Incubate with ab227643 for 10 minutes at room temperature.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "Boil tissue section in 10mM citrate, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Incubate with ab227643 for 10 minutes at room temperature.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/200", "IHCFr-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "Boil tissue section in 10mM citrate, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Incubate with ab227643 for 10 minutes at room temperature.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/200", "IHCFr-species-notes": "" }, "Cow": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS by protein A/G.

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KRT6C also known as Keratin 6C is a type II cytokeratin involved mechanically in structural support and protection in epithelial tissues. The protein has a molecular mass of approximately 59-60 kDa. It is mainly expressed in stratified epithelia including the skin mucosal linings and other keratinized surfaces. KRT6C provides resilience and strength to cells by forming keratin intermediate filaments that create a robust cytoskeletal network.

Biological function summary

Within various stratified epithelial tissues KRT6C ensures cellular integrity and facilitates protection against mechanical stress. As part of the keratin intermediate filament protein family KRT6C often interacts with other keratins typically forming complexes with KRT16 and KRT17 which enhance cell stability and responsiveness to damage. This protein plays a role in wound healing and cellular proliferation.

Pathways

KRT6C participates in several key biological processes including wound healing and the stress response pathway. In these pathways KRT6C functions closely with other proteins such as KRT16 which modulates cellular responses to environmental stressors and helps repair the epithelial barrier. The integration of KRT6C in these pathways highlights its importance in maintaining epithelial health and facilitating rapid response to injury.

KRT6C has significant connections with pachyonychia congenita a rare genetic disorder characterized by nail dystrophy and thickened skin. This condition often involves mutations in the KRT6C gene disrupting its interaction with KRT16 and KRT17 which compromises structural integrity of the skin and nail tissues. Furthermore KRT6C has implications in psoriasis a chronic inflammatory skin disorder where its altered expression may influence disease progression or severity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information KRT6C

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology 38:2867-2880 PubMed37565747

2023

Assessing the double-edged of extracellular signal-regulated kinase/CCAAT-enhancer-binding protein beta signaling pathway in arsenic-induced skin damage and its potential foodborne interventions.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Yang,Dexiu Hu,Sufei Du,Liping Wu,Maoyuan Gong,Yuhong Zhang,Xingcan Yang,Yang Yang,Ruobi Chen,Yuyan Xu,Qibing Zeng

Science advances 5:eaax5851 PubMed31633026

2019

MIBI-TOF: A multiplexed imaging platform relates cellular phenotypes and tissue structure.

Applications

Mass Cytometry

Species

Unspecified reactive species

Leeat Keren,Marc Bosse,Steve Thompson,Tyler Risom,Kausalia Vijayaragavan,Erin McCaffrey,Diana Marquez,Roshan Angoshtari,Noah F Greenwald,Harris Fienberg,Jennifer Wang,Neeraja Kambham,David Kirkwood,Garry Nolan,Thomas J Montine,Stephen J Galli,Robert West,Sean C Bendall,Michael Angelo

Cell 174:1373-1387.e19 PubMed30193111

2018

A Structured Tumor-Immune Microenvironment in Triple Negative Breast Cancer Revealed by Multiplexed Ion Beam Imaging.

Applications

Mass Cytometry

Species

Unspecified reactive species

Leeat Keren,Marc Bosse,Diana Marquez,Roshan Angoshtari,Samir Jain,Sushama Varma,Soo-Ryum Yang,Allison Kurian,David Van Valen,Robert West,Sean C Bendall,Michael Angelo

PloS one 10:e0125255 PubMed25955027

2015

Initiation and characterization of small cell lung cancer patient-derived xenografts from ultrasound-guided transbronchial needle aspirates.

Applications

Unspecified application

Species

Unspecified reactive species

Wade C Anderson,Michael B Boyd,Jorge Aguilar,Brett Pickell,Amy Laysang,Marybeth A Pysz,Sheila Bheddah,Johanna Ramoth,Brian C Slingerland,Scott J Dylla,Edmundo R Rubio

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KRT6C antibody [SP87]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

Flow Cytometry (Intracellular) - Anti-KRT6C antibody [SP87] (AB227643)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

Flow Cytometry (Intracellular) - Anti-KRT6C antibody [SP87] (AB227643)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

Immunocytochemistry/ Immunofluorescence - Anti-KRT6C antibody [SP87] (AB227643)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

Immunohistochemistry (Frozen sections) - Anti-KRT6C antibody [SP87] (AB227643)

Immunohistochemistry (Frozen sections) - Anti-KRT6C antibody [SP87] (AB227643)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] (AB227643)

Western blot - Anti-KRT6C antibody [SP87] (AB227643)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise