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AB240985

Anti-KRT6C antibody [SP87] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal K2C6C antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P, IHC-Fr, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

KRT6E, KRT6C, Cytokeratin-6C, Cytokeratin-6E, Keratin K6h, Keratin-6C, Type-II keratin Kb12, CK-6C, CK-6E, K6C

19 Images
Flow Cytometry (Intracellular) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)

Intracellular flow cytometric analysis of A431 (human epidermoid carcinoma cell line) cell line labeling KRT6C with ab227643 at 1/100 dilution (green) compared with a negative control rabbit IgG (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)

Formalin-fixed, paraffin-embedded human stomach tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)

Formalin-fixed, paraffin-embedded human rectal adenocarcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)

Flow Cytometry (Intracellular) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)

Intracellular Flow Cytometry analysis of HaCaT (human skin keratinocyte) labeling KRT6C with purified ab227643 at 1/200 dilution (0.48μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor®488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)

Formalin-fixed, paraffin-embedded human placenta tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)

Formalin-fixed, paraffin-embedded human bladder tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)

Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)

Immunocytochemistry/ Immunofluorescence - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)

Immunocytochemistry/ Immunofluorescence analysis of HaCaT (human skin keratinocyte) cells labeling KRT6C with purified ab227643 at 1/50 (1.92 μg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)

Formalin-fixed, paraffin-embedded human prostate tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)

Formalin-fixed, paraffin-embedded human breast tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)

This data was developed using the same antibody clone in a different buffer formulation (ab227643).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate carcinoma tissue sections labeling KRT6C with ab227643 at 1/200 dilution (0.12 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Leica DS9800 (Bond™ Polymer Refine Detection) was used as a secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human prostate carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227643 for 10 mins at room temperature.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)

Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)

This data was developed using the same antibody clone in a different buffer formulation (ab227643).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat skin tissue sections labeling KRT6C with ab227643 at 1/200 dilution (0.12 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Leica DS9800 (Bond™ Polymer Refine Detection) was used as a secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the rat skin, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227643 for 10 mins at room temperature.

Immunohistochemistry (Frozen sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)

Immunohistochemistry (Frozen) analysis of rat skin tissue section labeling KRT6C with purified ab227643 at 1/25 (3.84μg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643).

Immunohistochemistry (Frozen sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)

Immunohistochemistry (Frozen) analysis of mouse skin tissue section labeling KRT6C with purified ab227643 at 1/25 (3.84μg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (AB240985)

This data was developed using the same antibody clone in a different buffer formulation (ab227643).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse skin tissue sections labeling KRT6C with ab227643 at 1/200 dilution (0.12 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Leica DS9800 (Bond™ Polymer Refine Detection) was used as a secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the mouse skin, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227643 for 10 mins at room temperature.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP87

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), ICC/IF, IHC-Fr, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p>Boil tissue section in 10mM citrate, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Incubate with <a href='/en-us/products/primary-antibodies/krt6c-antibody-sp87-ab227643'>ab227643</a> for 10 minutes at room temperature.</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p>Primary antibody incubation for 30 minutes at 4°C.</p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p>Boil tissue section in 10mM citrate, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Incubate with <a href='/en-us/products/primary-antibodies/krt6c-antibody-sp87-ab227643'>ab227643</a> for 10 minutes at room temperature.</p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p>Boil tissue section in 10mM citrate, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Incubate with <a href='/en-us/products/primary-antibodies/krt6c-antibody-sp87-ab227643'>ab227643</a> for 10 minutes at room temperature.</p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab240985 is the carrier-free version of ab227643.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by protein A/G.
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KRT6C also known as Keratin 6C is a type II cytokeratin involved mechanically in structural support and protection in epithelial tissues. The protein has a molecular mass of approximately 59-60 kDa. It is mainly expressed in stratified epithelia including the skin mucosal linings and other keratinized surfaces. KRT6C provides resilience and strength to cells by forming keratin intermediate filaments that create a robust cytoskeletal network.
Biological function summary

Within various stratified epithelial tissues KRT6C ensures cellular integrity and facilitates protection against mechanical stress. As part of the keratin intermediate filament protein family KRT6C often interacts with other keratins typically forming complexes with KRT16 and KRT17 which enhance cell stability and responsiveness to damage. This protein plays a role in wound healing and cellular proliferation.

Pathways

KRT6C participates in several key biological processes including wound healing and the stress response pathway. In these pathways KRT6C functions closely with other proteins such as KRT16 which modulates cellular responses to environmental stressors and helps repair the epithelial barrier. The integration of KRT6C in these pathways highlights its importance in maintaining epithelial health and facilitating rapid response to injury.

KRT6C has significant connections with pachyonychia congenita a rare genetic disorder characterized by nail dystrophy and thickened skin. This condition often involves mutations in the KRT6C gene disrupting its interaction with KRT16 and KRT17 which compromises structural integrity of the skin and nail tissues. Furthermore KRT6C has implications in psoriasis a chronic inflammatory skin disorder where its altered expression may influence disease progression or severity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information KRT6C
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular cancer 20:123 PubMed34579723

2021

p113 isoform encoded by CUX1 circular RNA drives tumor progression via facilitating ZRF1/BRD4 transactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Yang,Anpei Hu,Yanhua Guo,Jianqun Wang,Dan Li,Xiaojing Wang,Shikai Jin,Boling Yuan,Shuang Cai,Yi Zhou,Qilan Li,Guo Chen,Haiyang Gao,Liduan Zheng,Qiangsong Tong
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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