Rabbit Recombinant Monoclonal K2C6C antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P, IHC-Fr, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
pH: 7.2 - 7.4
Constituents: PBS
ICC/IF | WB | IHC-P | IHC-Fr | Flow Cyt (Intra) | |
---|---|---|---|---|---|
Human | Tested | Tested | Tested | Expected | Tested |
Mouse | Expected | Expected | Tested | Tested | Expected |
Rat | Expected | Expected | Tested | Tested | Expected |
Cow | Predicted | Predicted | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/200 | Notes Boil tissue section in 10mM citrate, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Incubate with Anti-KRT6C antibody [SP87] ab227643 for 10 minutes at room temperature. |
Species Rat | Dilution info 1/200 | Notes Boil tissue section in 10mM citrate, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Incubate with Anti-KRT6C antibody [SP87] ab227643 for 10 minutes at room temperature. |
Species Human | Dilution info 1/200 | Notes Boil tissue section in 10mM citrate, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Incubate with Anti-KRT6C antibody [SP87] ab227643 for 10 minutes at room temperature. |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Primary antibody incubation for 30 minutes at 4°C. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info - | Notes - |
KRT6E, KRT6C, Cytokeratin-6C, Cytokeratin-6E, Keratin K6h, Keratin-6C, Type-II keratin Kb12, CK-6C, CK-6E, K6C
Rabbit Recombinant Monoclonal K2C6C antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P, IHC-Fr, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
pH: 7.2 - 7.4
Constituents: PBS
Purified from TCS by protein A/G.
ab240985 is the carrier-free version of Anti-KRT6C antibody [SP87] ab227643.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
KRT6C also known as Keratin 6C is a type II cytokeratin involved mechanically in structural support and protection in epithelial tissues. The protein has a molecular mass of approximately 59-60 kDa. It is mainly expressed in stratified epithelia including the skin mucosal linings and other keratinized surfaces. KRT6C provides resilience and strength to cells by forming keratin intermediate filaments that create a robust cytoskeletal network.
Within various stratified epithelial tissues KRT6C ensures cellular integrity and facilitates protection against mechanical stress. As part of the keratin intermediate filament protein family KRT6C often interacts with other keratins typically forming complexes with KRT16 and KRT17 which enhance cell stability and responsiveness to damage. This protein plays a role in wound healing and cellular proliferation.
KRT6C participates in several key biological processes including wound healing and the stress response pathway. In these pathways KRT6C functions closely with other proteins such as KRT16 which modulates cellular responses to environmental stressors and helps repair the epithelial barrier. The integration of KRT6C in these pathways highlights its importance in maintaining epithelial health and facilitating rapid response to injury.
KRT6C has significant connections with pachyonychia congenita a rare genetic disorder characterized by nail dystrophy and thickened skin. This condition often involves mutations in the KRT6C gene disrupting its interaction with KRT16 and KRT17 which compromises structural integrity of the skin and nail tissues. Furthermore KRT6C has implications in psoriasis a chronic inflammatory skin disorder where its altered expression may influence disease progression or severity.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using the same antibody clone in a different buffer formulation (Anti-KRT6C antibody [SP87] ab227643).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate carcinoma tissue sections labeling KRT6C with Anti-KRT6C antibody [SP87] ab227643 at 1/200 dilution (0.12 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Leica DS9800 (Bond™ Polymer Refine Detection) was used as a secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human prostate carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with Anti-KRT6C antibody [SP87] ab227643 for 10 mins at room temperature.
Immunocytochemistry/ Immunofluorescence analysis of HaCaT (human skin keratinocyte) cells labeling KRT6C with purified Anti-KRT6C antibody [SP87] ab227643 at 1/50 (1.92 μg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KRT6C antibody [SP87] ab227643).
Intracellular Flow Cytometry analysis of HaCaT (human skin keratinocyte) labeling KRT6C with purified Anti-KRT6C antibody [SP87] ab227643 at 1/200 dilution (0.48μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black). Unlableled control - Unlabelled cells (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KRT6C antibody [SP87] ab227643).
This data was developed using the same antibody clone in a different buffer formulation (Anti-KRT6C antibody [SP87] ab227643).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat skin tissue sections labeling KRT6C with Anti-KRT6C antibody [SP87] ab227643 at 1/200 dilution (0.12 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Leica DS9800 (Bond™ Polymer Refine Detection) was used as a secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the rat skin, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with Anti-KRT6C antibody [SP87] ab227643 for 10 mins at room temperature.
This data was developed using the same antibody clone in a different buffer formulation (Anti-KRT6C antibody [SP87] ab227643).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse skin tissue sections labeling KRT6C with Anti-KRT6C antibody [SP87] ab227643 at 1/200 dilution (0.12 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Leica DS9800 (Bond™ Polymer Refine Detection) was used as a secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the mouse skin, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with Anti-KRT6C antibody [SP87] ab227643 for 10 mins at room temperature.
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for KRT6C using Anti-KRT6C antibody [SP87] ab227643 at 1/200 dilution in immunohistochemical analysis.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KRT6C antibody [SP87] ab227643)
Immunohistochemistry (Frozen) analysis of rat skin tissue section labeling KRT6C with purified Anti-KRT6C antibody [SP87] ab227643 at 1/25 (3.84μg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KRT6C antibody [SP87] ab227643).
Immunohistochemistry (Frozen) analysis of mouse skin tissue section labeling KRT6C with purified Anti-KRT6C antibody [SP87] ab227643 at 1/25 (3.84μg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KRT6C antibody [SP87] ab227643).
Intracellular flow cytometric analysis of A431 (human epidermoid carcinoma cell line) cell line labeling KRT6C with Anti-KRT6C antibody [SP87] ab227643 at 1/100 dilution (green) compared with a negative control rabbit IgG (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KRT6C antibody [SP87] ab227643).
Formalin-fixed, paraffin-embedded human breast tissue stained for KRT6C using Anti-KRT6C antibody [SP87] ab227643 at 1/200 dilution in immunohistochemical analysis.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KRT6C antibody [SP87] ab227643)
Formalin-fixed, paraffin-embedded human stomach tissue stained for KRT6C using Anti-KRT6C antibody [SP87] ab227643 at 1/200 dilution in immunohistochemical analysis.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KRT6C antibody [SP87] ab227643)
Formalin-fixed, paraffin-embedded human prostate tissue stained for KRT6C using Anti-KRT6C antibody [SP87] ab227643 at 1/200 dilution in immunohistochemical analysis.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KRT6C antibody [SP87] ab227643)
Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for KRT6C using Anti-KRT6C antibody [SP87] ab227643 at 1/200 dilution in immunohistochemical analysis.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KRT6C antibody [SP87] ab227643)
Formalin-fixed, paraffin-embedded human rectal adenocarcinoma tissue stained for KRT6C using Anti-KRT6C antibody [SP87] ab227643 at 1/200 dilution in immunohistochemical analysis.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KRT6C antibody [SP87] ab227643)
Formalin-fixed, paraffin-embedded human bladder tissue stained for KRT6C using Anti-KRT6C antibody [SP87] ab227643 at 1/200 dilution in immunohistochemical analysis.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KRT6C antibody [SP87] ab227643)
Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for KRT6C using Anti-KRT6C antibody [SP87] ab227643 at 1/200 dilution in immunohistochemical analysis.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KRT6C antibody [SP87] ab227643)
Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for KRT6C using Anti-KRT6C antibody [SP87] ab227643 at 1/200 dilution in immunohistochemical analysis.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KRT6C antibody [SP87] ab227643)
Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for KRT6C using Anti-KRT6C antibody [SP87] ab227643 at 1/200 dilution in immunohistochemical analysis.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KRT6C antibody [SP87] ab227643)
Formalin-fixed, paraffin-embedded human placenta tissue stained for KRT6C using Anti-KRT6C antibody [SP87] ab227643 at 1/200 dilution in immunohistochemical analysis.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KRT6C antibody [SP87] ab227643)
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com