AB55407

Anti-KRT81 antibody [3B10-5B10]

Name: Anti-KRT81 antibody [3B10-5B10] (ab55407) | Abcam
Brand: Abcam Limited
SKU: AB55407
Price: 715 USD
Availability: InStock

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(4 Publications)

Mouse Monoclonal KRT81 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human KRT81.

View Alternative Names

KRTHB1, MLN137, KRT81, Hair keratin K2.9, Keratin-81, Metastatic lymph node 137 gene protein, Type II hair keratin Hb1, Type-II keratin Kb21, ghHKb1, K81, MLN 137, ghHb1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT81 antibody [3B10-5B10] (AB55407)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT81 antibody [3B10-5B10] (AB55407)

KRT81 antibody (ab55407) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human esophagus. This was producted using the ascites version of the product.

Immunocytochemistry/ Immunofluorescence - Anti-KRT81 antibody [3B10-5B10] (AB55407)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-KRT81 antibody [3B10-5B10] (AB55407)

KRT81 antibody (ab55407) used in immunofluorescence at 10ug/ml on HeLa cells. This was producted using the ascites version of the product.

Unknown

Western blot - Anti-KRT81 antibody [3B10-5B10] (AB55407)

KRT81 antibody (ab55407) at 1ug/lane + HepG2 cell lysate at 25ug/lane. This was producted using the ascites version of the product.

All lanes:

Western blot - Anti-KRT81 antibody [3B10-5B10] (ab55407)

Predicted band size: 192 kDa,55 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3B10-5B10

Isotype

IgG2b

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human KRT81.

Q14533

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 27/June/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Purification notes

Purified from TCS.

Storage buffer

pH: 7.4

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KRT81 also known as Keratin 81 or Hair Keratin K2.9 is a type II keratin protein with a molecular mass of approximately 55 kDa. This protein is mechanical forming intermediate filaments that provide structural support in the cells. KRT81 is primarily found in hair follicles especially in the cortex of hair fibers where it plays a role in the formation and stabilization of hair structure. Its highly regulated expression is critical during hair development and growth.

Biological function summary

KRT81 contributes to the rigidity and integrity of hair by interacting within a keratin complex. This complex includes other keratin proteins that support the polymerization process needed for filament formation. The presence of KRT81 in non-dividing differentiating cells in the hair follicle indicates its important role during the hair cycle stages ensuring the mechanical strength needed for protective and sensory functions.

Pathways

Several biological processes depend on the structural role of KRT81. It is part of the keratinization pathway which is essential for hair formation impacting the assembly of hair alongside other keratin proteins such as KRT31 and KRT35. These proteins work together to create the strong keratin structure necessary for healthy hair development. KRT81 interacts within a network that influences hair shaft differentiation.

KRT81 mutations can lead to hair-related conditions. One such disorder is Monilethrix characterized by beaded hair and hair shaft abnormalities. In Monilethrix changes in KRT81 can disrupt the protein network impacting other keratins like KRT83 and leading to fragile hair. Understanding these connections helps in developing therapies aimed at treating hair-related disorders by targeting specific points in the keratin protein network.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information KRT81

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:77 PubMed39819676

2025

Single-cell transcriptomic analysis reveals characteristic feature of macrophage reprogramming in liver Mallory-Denk bodies pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Zixuan Fang,Bei Zhong,Yi Shi,Wanmei Zhou,Maoping Huang,Samuel W French,Xiaoping Tang,Hui Liu

Molecular cancer 21:133 PubMed35733218

2022

Single-cell RNA-seq reveals the genesis and heterogeneity of tumor microenvironment in pancreatic undifferentiated carcinoma with osteoclast-like giant-cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xinbo Wang,Jiaying Miao,Sizhen Wang,Rongxi Shen,Shuo Zhang,Yurao Tian,Min Li,Daojun Zhu,Anlong Yao,Wei Bao,Qun Zhang,Xingming Tang,Xingyun Wang,Jieshou Li

Theranostics 11:5650-5674 PubMed33897873

2021

Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Zhang,Keke Yu,Lifang Ma,Zijun Qian,Xiaoting Tian,Yayou Miao,Yongjie Niu,Xin Xu,Susu Guo,Yueyue Yang,Zhixian Wang,Xiangfei Xue,Chuanjia Gu,Wentao Fang,Jiayuan Sun,Yongchun Yu,Jiayi Wang

Molecular medicine reports 13:2159-66 PubMed26781175

2016

TLR4‑dependent signaling pathway modulation: A novel mechanism by which pioglitazone protects against nutritional fibrotic steatohepatitis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghua Du,Xuemin Niu,Rongqi Wang,Suxian Zhao,Lingbo Kong,Yuguo Zhang,Yuemin Nan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KRT81 antibody [3B10-5B10]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT81 antibody [3B10-5B10] (AB55407)

Immunocytochemistry/ Immunofluorescence - Anti-KRT81 antibody [3B10-5B10] (AB55407)

Western blot - Anti-KRT81 antibody [3B10-5B10] (AB55407)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise