Rabbit Polyclonal KRTHA3B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human KRT33B aa 100 to C-terminus.
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Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human KRT33B aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q14525
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Alternatively Tris-EDTA buffer (pH8.0) may be used. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000.00000 - 1/20000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Alternative names
Keratin-33B, K33B, HHA3-II, KRT33B, HKA3B, KRTHA3B
Recommended products
Rabbit Polyclonal KRTHA3B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human KRT33B aa 100 to C-terminus.
Alternative names
Keratin-33B, K33B, HHA3-II, KRT33B, HKA3B, KRTHA3B
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human KRT33B aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q14525
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KRTHA3B also known as keratin 83 is a type II hair keratin protein with a molecular mass of approximately 50 kDa. This protein functions mechanically as a structural component in the assembly of intermediate filaments in hair and nail tissues. KRTHA3B forms a critical part of the cytoskeleton in these cells conferring resilience and structural integrity. It expresses prominently in hair follicles and nail beds which is essential for maintaining the strength and rigidity of keratinized tissues.
- Biological function summary
Keratins like KRTHA3B are key in building the fibrous framework necessary for tissue stability and function in epithelial cells. KRTHA3B associates with other keratin proteins forming complex networks that are involved in the mechanical support of epithelial cells. Such networks maintain the shape and mechanical resistance required by the cells particularly in the rapidly growing and dividing cells in hair follicles. This protein contributes to filament stabilization ensuring that the structure remains tight and resistant to physical stress.
- Pathways
Keratin proteins including KRTHA3B play essential roles in epithelial cell differentiation and structural maintenance. They integrate into pathways such as epithelial-mesenchymal transition (EMT) and cell adhesion playing roles in cellular adhesion dynamics. Related proteins include keratin 19 and keratin 14 which participate together to maintain epithelial cell integrity and function. These pathways ensure structural stability and reliable barrier functions in tissues.
- Associated diseases and disorders
The mutation or deregulation of KRTHA3B can associate with conditions such as hair disorders and some nail dystrophies. Keratin-related disorders often result from dysfunctional filament assembly or structure affecting the affected tissues' resilience. Connected proteins in these disorders include keratin 5 and keratin 17 which are involved in similar filament assembly processes and can contribute to appearance and functionality defects when mutated or expressed incorrectly.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-KRTHA3B antibody (ab154339)
10% SDS PAGE
All lanes: Western blot - Anti-KRTHA3B antibody (ab154339) at 1/10000 dilution
All lanes: HeLa whole cell lysate at 30 µg
Predicted band size: 24 kDa, 46 kDa
Immunocytochemistry/ Immunofluorescence - Anti-KRTHA3B antibody (ab154339)
Immunofluorescence analysis of methanol-fixed HeLa cells labeling KRTHA3B with ab154339 at 1/500 dilution (green) and counterstained with Hoechst 3342 in bottom panel (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRTHA3B antibody (ab154339)
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, labeling KRTHA3B with ab154339 at 1/500 dilution
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