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AB154339

Anti-KRTHA3B antibody

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(1 Publication)

Rabbit Polyclonal KRTHA3B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Dog samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human KRT33B aa 100 to C-terminus.

View Alternative Names

HHA3-II, HKA3B, KRTHA3B, KRT33B, Keratin-33B, K33B

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRTHA3B antibody (AB154339)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRTHA3B antibody (AB154339)

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, labeling KRTHA3B with ab154339 at 1/500 dilution

Immunocytochemistry/ Immunofluorescence - Anti-KRTHA3B antibody (AB154339)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-KRTHA3B antibody (AB154339)

Immunofluorescence analysis of methanol-fixed HeLa cells labeling KRTHA3B with ab154339 at 1/500 dilution (green) and counterstained with Hoechst 3342 in bottom panel (blue).

Western blot - Anti-KRTHA3B antibody (AB154339)
  • WB

Unknown

Western blot - Anti-KRTHA3B antibody (AB154339)

10% SDS PAGE

All lanes:

Western blot - Anti-KRTHA3B antibody (ab154339) at 1/10000 dilution

All lanes:

HeLa whole cell lysate at 30 µg

Predicted band size: 24 kDa,46 kDa

false

Western blot - Anti-KRTHA3B antibody (AB154339)
  • WB

Supplier Data

Western blot - Anti-KRTHA3B antibody (AB154339)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-KRTHA3B antibody (ab154339) at 1/10000 dilution

All lanes:

MDCK Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Dog

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human KRT33B aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q14525

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Alternatively Tris-EDTA buffer (pH8.0) may be used.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Dog": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KRTHA3B also known as keratin 83 is a type II hair keratin protein with a molecular mass of approximately 50 kDa. This protein functions mechanically as a structural component in the assembly of intermediate filaments in hair and nail tissues. KRTHA3B forms a critical part of the cytoskeleton in these cells conferring resilience and structural integrity. It expresses prominently in hair follicles and nail beds which is essential for maintaining the strength and rigidity of keratinized tissues.
Biological function summary

Keratins like KRTHA3B are key in building the fibrous framework necessary for tissue stability and function in epithelial cells. KRTHA3B associates with other keratin proteins forming complex networks that are involved in the mechanical support of epithelial cells. Such networks maintain the shape and mechanical resistance required by the cells particularly in the rapidly growing and dividing cells in hair follicles. This protein contributes to filament stabilization ensuring that the structure remains tight and resistant to physical stress.

Pathways

Keratin proteins including KRTHA3B play essential roles in epithelial cell differentiation and structural maintenance. They integrate into pathways such as epithelial-mesenchymal transition (EMT) and cell adhesion playing roles in cellular adhesion dynamics. Related proteins include keratin 19 and keratin 14 which participate together to maintain epithelial cell integrity and function. These pathways ensure structural stability and reliable barrier functions in tissues.

The mutation or deregulation of KRTHA3B can associate with conditions such as hair disorders and some nail dystrophies. Keratin-related disorders often result from dysfunctional filament assembly or structure affecting the affected tissues' resilience. Connected proteins in these disorders include keratin 5 and keratin 17 which are involved in similar filament assembly processes and can contribute to appearance and functionality defects when mutated or expressed incorrectly.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information KRT33B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 9:19089 PubMed31836741

2019

MicroRNA-509-3p inhibits cellular migration, invasion, and proliferation, and sensitizes osteosarcoma to cisplatin.

Applications

Unspecified application

Species

Unspecified reactive species

Sagar L Patil,Asha Palat,Yinghong Pan,Kimal Rajapakshe,Rachna Mirchandani,Maria Bondesson,Jason T Yustein,Cristian Coarfa,Preethi H Gunaratne
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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