AB174272

Anti-KRTHB3 antibody [EPR12333]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal KRTHB3 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

KRTHB3, KRT83, Hair keratin K2.10, Keratin-83, Type II hair keratin Hb3, Type-II keratin Kb23, K83

1 Images

Supplier Data

Western blot - Anti-KRTHB3 antibody [EPR12333] (AB174272)

All lanes:

Western blot - Anti-KRTHB3 antibody [EPR12333] (ab174272) at 1/1000 dilution

Lane 1:

Human skin lysate at 10 µg

Lane 2:

C6 lysate at 10 µg

Lane 3:

NIH 3T3 lysate at 10 µg

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12333

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KRTHB3 also known as Type I Hair Keratin 3 is a structural protein that plays an integral role in the formation of hair and nail cells. Its molecular mass is approximately 66 kDa. It is expressed in hair follicles and nails where it contributes to the robustness and integrity of these tissues. The protein is a component of the intermediate filament family which provides mechanical strength to cells by forming part of the cytoskeleton.

Biological function summary

The functional units of hair and nail cells utilize KRTHB3 as part of the cytoskeletal framework ensuring the structural integrity essential for normal growth and development. KRTHB3 is also an important component of the keratin filament network which forms a fibrous matrix. It often works in tandem with other keratin proteins such as KRTHB1 and KRTHB2 forming heterodimers that create a durable structure in keratinized tissues.

Pathways

KRTHB3 operates as a constituent of the keratinization process and plays a major role in the epithelial cell differentiation pathway. These pathways are important for the development of hair nails and skin. In these processes KRTHB3 interacts with other structural proteins like desmoplakin and envoplakin which are integral for cell adhesion and forming the epidermis barrier.

KRTHB3 mutations or dysregulation can relate to conditions like alopecia and nail dystrophy. Abnormalities in the expression of KRTHB3 can lead to weakened hair or nail structures due to improper keratin formation. In some cases these conditions may also involve interactions with other proteins such as filaggrin a protein known to contribute to skin barrier function and hydration exacerbating the severity of these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information KRT83

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of microbiology and biotechnology 32:938-948 PubMed35791074

2022

CircCOL1A2 Sponges MiR-1286 to Promote Cell Invasion and Migration of Gastric Cancer by Elevating Expression of USP10 to Downregulate RFC2 Ubiquitination Level.

Applications

Unspecified application

Species

Unspecified reactive species

Hang Li,Lixin Chai,Zujun Ding,Huabo He

Amino acids 54:591-600 PubMed34181092

2021

Love is in the hair: arginine methylation of human hair proteins as novel cardiovascular biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

Alistair James Marsden,David R J Riley,Stefan Birkett,Quentin Rodriguez-Barucg,Barbara-Ann Guinn,Sean Carroll,Lee Ingle,Thozhukat Sathyapalan,Pedro Beltran-Alvarez

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KRTHB3 antibody [EPR12333]

Western blot - Anti-KRTHB3 antibody [EPR12333] (AB174272)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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