AB36618

Anti-KSHV ORF45 antibody [2D4A5]

Name: Anti-KSHV ORF45 antibody [2D4A5] (ab36618) | Abcam
Brand: Abcam Limited
SKU: AB36618

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(7 Publications)

Mouse Monoclonal KSHV ORF45 antibody. Suitable for WB, ICC, ELISA and reacts with Recombinant fragment - Human gammaherpesvirus 8, Human gammaherpesvirus 8 samples. Cited in 7 publications.

2 Images

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Immunocytochemistry - Anti-KSHV ORF45 antibody [2D4A5] (AB36618)

Immunochemistry staining of TPA-induced BCBL-1 cells, using ab36618. HRP-anti-mouse was used as the secondary antibody before colour development with DAB.

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Western blot - Anti-KSHV ORF45 antibody [2D4A5] (AB36618)

Based on the analysis of the mRNA sequence, ORF45 is expected to encode a protein of 407 amino acids with a molecular mass of about 50 kDa. However, ORF45 was displayed as a diffuse band at the position of 78 kDa on a Western blot after SDS-PAGE. This observation implied that ORF45 might be highly post-translationally modified, resulting in its reduced mobility in denaturing SDS-PAGE. A survey of the primary amino acid sequence of ORF45 revealed multiple potential sites of phosphorylation, including a serine-rich region and other consensus sites for phosphorylation by the serine/threonine kinase, casein kinase II, and protein kinase C (Zhu & Yuan, 2003).

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Western blot - Anti-KSHV ORF45 antibody [2D4A5] (ab36618)

Predicted band size: 43 kDa

Observed band size: 78 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2D4A5

Isotype

IgG1

Carrier free

Reacts with

Human gammaherpesvirus 8

Applications

WB, ICC, ELISA

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human gammaherpesvirus 8": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/200 - 1/1000", "ICC-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/10000", "ELISA-species-notes": "" }, "Recombinant fragment - Human gammaherpesvirus 8": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KSHV ORF45 also known as Kaposi’s sarcoma-associated herpesvirus ORF45 functions as an immediate-early protein. Researchers have identified its molecular weight around 48 kDa. It expresses in the cytoplasm of infected cells during the lytic cycle of KSHV a human herpesvirus. This protein inhibits the antiviral response by interacting with host cell signaling pathways making it an important component of the virus's lifecycle.

Biological function summary

The function of KSHV ORF45 impacts cellular processes that are essential for viral replication and immune evasion. This protein interacts with several signaling molecules such as RSK and ERK which are part of the host cell's defense mechanisms. It prevents cellular apoptosis and supports viral gene expression by inhibiting the host's innate immune response. KSHV ORF45 does not exist as part of a larger protein complex but operates through direct protein-protein interactions.

Pathways

Research shows that KSHV ORF45 modifies the MAPK/ERK signaling pathway. This pathway is critical for cell proliferation and survival aiding the virus in evading host immunity and establishing infection. The protein closely links with RSK1/2 important in transducing ERK signals. This modification affects the host cell's ability to mount an effective antiviral response supporting viral pathogenesis.

Delineating the role of KSHV ORF45 connects it to Kaposi's sarcoma and primary effusion lymphoma. These malignancies occur frequently in individuals with compromised immune systems like those with AIDS. KSHV ORF45 indirectly links with proteins such as LANA and vIL-6 which contribute to cellular transformation and oncogenesis. Understanding this protein’s role offers insights into potential therapeutic targets for KSHV-related malignancies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

See full target information KSHV ORF45

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:4125 PubMed38374336

2024

Pacritinib inhibits proliferation of primary effusion lymphoma cells and production of viral interleukin-6 induced cytokines.

Applications

Unspecified application

Species

Unspecified reactive species

Yiquan Wu,Victoria Wang,Robert Yarchoan

Journal of virology 91: PubMed28515293

2017

Virus-Like Vesicles of Kaposi's Sarcoma-Associated Herpesvirus Activate Lytic Replication by Triggering Differentiation Signaling.

Applications

Species

Unspecified reactive species

Danyang Gong,Xinghong Dai,Yuchen Xiao,Yushen Du,Travis J Chapa,Jeffrey R Johnson,Xinmin Li,Nevan J Krogan,Hongyu Deng,Ting-Ting Wu,Ren Sun

Oncotarget 8:50342-50358 PubMed28881567

2017

Restoration of immune surface molecules in Kaposi sarcoma-associated herpes virus infected cells by lenalidomide and pomalidomide.

Applications

Species

Human

David A Davis,Suraj Mishra,Holda A Anagho,Ashley I Aisabor,Prabha Shrestha,Victoria Wang,Yuki Takamatsu,Kenji Maeda,Hiroaki Mitsuya,Jerome B Zeldis,Robert Yarchoan

PloS one 11:e0162574 PubMed27611973

2016

Profile of Exosomal and Intracellular microRNA in Gamma-Herpesvirus-Infected Lymphoma Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Shiho Hoshina,Tsuyoshi Sekizuka,Michiyo Kataoka,Hideki Hasegawa,Hiromichi Hamada,Makoto Kuroda,Harutaka Katano

mBio 6:e00668 PubMed26045540

2015

Kaposi Sarcoma Herpesvirus Induces HO-1 during De Novo Infection of Endothelial Cells via Viral miRNA-Dependent and -Independent Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Botto,Jennifer E Totonchy,Jean K Gustin,Ashlee V Moses

Journal of virology 86:12717-30 PubMed22973026

2012

MicroRNAs and unusual small RNAs discovered in Kaposi's sarcoma-associated herpesvirus virions.

Applications

Unspecified application

Species

Unspecified reactive species

Xianzhi Lin,Xiaofan Li,Deguang Liang,Ke Lan

Journal of virology 82:2230-40 PubMed18077711

2007

SIAH-1 interacts with the Kaposi's sarcoma-associated herpesvirus-encoded ORF45 protein and promotes its ubiquitylation and proteasomal degradation.

Applications

Species

Unspecified reactive species

Rinat Abada,Tsofia Dreyfuss-Grossman,Yifat Herman-Bachinsky,Haim Geva,Shiri-Rivka Masa,Ronit Sarid

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KSHV ORF45 antibody [2D4A5]

Immunocytochemistry - Anti-KSHV ORF45 antibody [2D4A5] (AB36618)

Western blot - Anti-KSHV ORF45 antibody [2D4A5] (AB36618)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise