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AB68483

Anti-KSR1 antibody [EPR2421Y]

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(9 Publications )

Anti-KSR1 antibody [EPR2421Y] (ab68483) is a rabbit monoclonal antibody detecting KSR1 in Western Blot, IHC-P. Suitable for Human, Mouse, Rat.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Trusted since 2008
6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KSR1 antibody [EPR2421Y] (AB68483)
  • IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KSR1 antibody [EPR2421Y] (AB68483)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue sections labeling KSR1 with Purified ab68483 at 1 : 100 dilution (1.49 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

Western blot - Anti-KSR1 antibody [EPR2421Y] (AB68483)
  • WB

Lab

Western blot - Anti-KSR1 antibody [EPR2421Y] (AB68483)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-KSR1 antibody [EPR2421Y] (ab68483) at 1/1000 dilution

All lanes:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 102 kDa

Observed band size: 110 kDa

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Western blot - Anti-KSR1 antibody [EPR2421Y] (AB68483)
  • WB

Lab

Western blot - Anti-KSR1 antibody [EPR2421Y] (AB68483)

Lane 1 : Wild-type HAP1 cell lysate (40 μg)
Lane 2 : KSR1 knockout HAP1 cell lysate (40 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : HEK293 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab68483 observed at 120 kDa. Red - loading control, ab8245, observed at 37 kDa.

Unpurified ab68483 was shown to recognize KSR1 when KSR1 knockout samples were used, along with additional cross-reactive bands. Wild-type and KSR1 knockout samples were subjected to SDS-PAGE. ab68483 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1 : 10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 : 10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-KSR1 antibody [EPR2421Y] (ab68483)

Predicted band size: 102 kDa

false

Western blot - Anti-KSR1 antibody [EPR2421Y] (AB68483)
  • WB

Lab

Western blot - Anti-KSR1 antibody [EPR2421Y] (AB68483)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-KSR1 antibody [EPR2421Y] (ab68483) at 1/1000 dilution

All lanes:

MEF (Mouse embryonic fibroblast (immortalized)) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 102 kDa

Observed band size: 110 kDa

false

Western blot - Anti-KSR1 antibody [EPR2421Y] (AB68483)
  • WB

Lab

Western blot - Anti-KSR1 antibody [EPR2421Y] (AB68483)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-KSR1 antibody [EPR2421Y] (ab68483) at 1/1000 dilution

All lanes:

Rat1 (Rat embryonic fibroblast) whole cell lysates

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 102 kDa

Observed band size: 110 kDa

false

Western blot - Anti-KSR1 antibody [EPR2421Y] (AB68483)
  • WB

Unknown

Western blot - Anti-KSR1 antibody [EPR2421Y] (AB68483)

All lanes:

Western blot - Anti-KSR1 antibody [EPR2421Y] (ab68483) at 1/2000 dilution

All lanes:

HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate at 10 µg

Secondary

All lanes:

goat anti rabbit HRP at 1/2000 dilution

Predicted band size: 102 kDa

Observed band size: 120 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2421Y

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-KSR1 antibody [EPR2421Y] (ab68483) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.

What is the molecular weight of KSR1?
Anti-KSR1 [EPR2421Y] (ab68483) specifically detects a band for KSR1 (UniProt: Q8IVT5) at a molecular weight of 102kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-KSR1 antibody [EPR2421Y] (ab68483) has been confirmed by Western blot testing in KSR1 Knockout HAP1 cells.

Other related products
We have a range of other formats of antibody clone [EPR2421Y] also available for your convenience: ab68483, Carrier free - ab227109, Alexa Fluor® 488 - ab320300, Alexa Fluor® 647 - ab320301, Alexa Fluor® 555 - ab320302, Alexa Fluor® 594 - ab320303, PE - ab320304, APC - ab320305, Alexa Fluor® 750 - ab321693

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KSR1 also known as Kinase Suppressor of Ras 1 serves as an important scaffold protein in cellular signaling. Weighing around 87 kDa KSR1 localizes in both the cytoplasm and the membrane of cells and shows expression in various tissues including the brain lung and ovaries. KSR1 does not have kinase activity itself but influences the activity of other kinases through its interactions.
Biological function summary

KSR1 exerts a regulatory role by organizing multi-protein complexes necessary for effective Ras and Raf signaling. This protein helps assemble the mitogen-activated protein kinase (MAPK) cascade complex which plays an essential role in cell growth and differentiation. As an important scaffolding component KSR1 enhances the efficiency of signal transduction pathways by facilitating the spatial organization of kinases involved in MAPK/ERK signaling.

Pathways

Signaling through KSR1 significantly impacts the MAPK/ERK and Ras pathways both of which are important for transmitting extracellular signals to the nucleus. KSR1 interacts with c-Raf MEK1/2 and ERK1/2 within these pathways forming a bridge that allows effective transduction of signals necessary for various cellular responses. By providing a platform for these kinases KSR1 ensures precise regulation of the signaling processes that control cell proliferation and survival.

Dysfunction in KSR1 activity associates with cancer and neurodegenerative disorders. KSR1 aberrations can contribute to oncogenic Ras-driven cancers due to its central role in controlling the Ras signaling pathway. Furthermore irregular KSR1 expression also links to neurodegenerative diseases through its impact on MAPK/ERK signaling. Proteins such as c-Raf and ERK1/2 which interact with KSR1 also play roles in these pathologies emphasizing the potential of KSR1 as a target for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of a multiprotein signaling complex which promotes phosphorylation of Raf family members and activation of downstream MAP kinases (By similarity). Independently of its kinase activity, acts as MAP2K1/MEK1 and MAP2K2/MEK2-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1 or MAP2K2/MEK2, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 and/or MAP2K2/MEK2 (PubMed : 29433126). Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP (By similarity). Its kinase activity is unsure (By similarity). Some protein kinase activity has been detected in vitro, however the physiological relevance of this activity is unknown (By similarity).
See full target information KSR1

Alternative Names

KSR, KSR1, Kinase suppressor of Ras 1
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Molecular oncology 19:452-473 PubMed39263917

2024

ERK1/2 mitogen-activated protein kinase dimerization is essential for the regulation of cell motility.

Applications

Unspecified application

Species

Unspecified reactive species

Dalia de la Fuente-Vivas,Vincenzo Cappitelli,Rocío García-Gómez,Sara Valero-Díaz,Camilla Amato,Javier Rodriguéz,Santiago Duro-Sánchez,Alexander von Kriegsheim,Michael Grusch,José Lozano,Joaquín Arribas,Berta Casar,Piero Crespo

Science advances 9:eadd7969 PubMed36791195

2023

Scaffold coupling: ERK activation by trans-phosphorylation across different scaffold protein species.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Martín-Vega,Laura Ruiz-Peinado,Rocío García-Gómez,Ana Herrero,Dalia de la Fuente-Vivas,Swetha Parvathaneni,Rubén Caloto,Marta Morante,Alex von Kriegsheim,Xosé R Bustelo,David B Sacks,Berta Casar,Piero Crespo

The Journal of biological chemistry 298:102121 PubMed35697074

2022

A novel antiproliferative PKCα-Ras-ERK signaling axis in intestinal epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Navneet Kaur,Michelle A Lum,Robert E Lewis,Adrian R Black,Jennifer D Black

Molecular oncology 16:3066-3081 PubMed35313064

2022

KSR induces RAS-independent MAPK pathway activation and modulates the efficacy of KRAS inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Guillem Paniagua,Harrys K C Jacob,Oksana Brehey,Sara García-Alonso,Carmen G Lechuga,Tirso Pons,Monica Musteanu,Carmen Guerra,Matthias Drosten,Mariano Barbacid

Journal of translational medicine 19:342 PubMed34372882

2021

ELK4 promotes the development of gastric cancer by inducing M2 polarization of macrophages through regulation of the KDM5A-PJA2-KSR1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zheng,Hongmei Xu,Ya Di,Lanlan Chen,Jiao Liu,Liying Kang,Liming Gao

eLife 10: PubMed33970103

2021

KSR1- and ERK-dependent translational regulation of the epithelial-to-mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Chaitra Rao,Danielle E Frodyma,Siddesh Southekal,Robert A Svoboda,Adrian R Black,Chittibabu Guda,Tomohiro Mizutani,Hans Clevers,Keith R Johnson,Kurt W Fisher,Robert E Lewis

The Journal of biological chemistry 296:100577 PubMed33766558

2021

Calmodulin influences MAPK signaling by binding KSR1.

Applications

Unspecified application

Species

Unspecified reactive species

Swetha Parvathaneni,Zhigang Li,David B Sacks

Acta biochimica et biophysica Sinica 50:807-816 PubMed30020400

2018

Effect of lentiviral vector-mediated KSR1 gene silencing on the proliferation of renal tubular epithelial cells and expression of inflammatory factors in a rat model of ischemia/reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Bai,Guanghong Han,Kaimin Guo,Lili Yu,Xiadong Du,Ying Xu

Nature 554:549-553 PubMed29433126

2018

MEK drives BRAF activation through allosteric control of KSR proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Hugo Lavoie,Malha Sahmi,Pierre Maisonneuve,Sara A Marullo,Neroshan Thevakumaran,Ting Jin,Igor Kurinov,Frank Sicheri,Marc Therrien
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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