Rabbit Recombinant Monoclonal KSR1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-KSR1 antibody [EPR2421Y] - BSA and Azide free (AB227109), expandable thumbnail](https://content.abcam.com/products/images/ksr1-antibody-epr2421y-bsa-and-azide-free-ab227109--western-blot-img143788.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-KSR1 antibody [EPR2421Y] - BSA and Azide free (AB227109)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KSR1 antibody [EPR2421Y] - BSA and Azide free (AB227109), expandable thumbnail](https://content.abcam.com/products/images/ksr1-antibody-epr2421y-bsa-and-azide-free-ab227109--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img45527.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KSR1 antibody [EPR2421Y] - BSA and Azide free (AB227109)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | Flow Cyt | WB | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Tested |
| Mouse | Expected | Not recommended | Not recommended | Not recommended | Tested |
| Rat | Expected | Not recommended | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse and rat samples. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Part of a multiprotein signaling complex which promotes phosphorylation of Raf family members and activation of downstream MAP kinases (By similarity). Independently of its kinase activity, acts as MAP2K1/MEK1 and MAP2K2/MEK2-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1 or MAP2K2/MEK2, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 and/or MAP2K2/MEK2 (PubMed:29433126). Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP (By similarity). Its kinase activity is unsure (By similarity). Some protein kinase activity has been detected in vitro, however the physiological relevance of this activity is unknown (By similarity).
Alternative names
KSR, KSR1, Kinase suppressor of Ras 1
Recommended products
Rabbit Recombinant Monoclonal KSR1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR2421Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab227109 is the carrier-free version of Anti-KSR1 antibody [EPR2421Y] ab68483.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KSR1 also known as Kinase Suppressor of Ras 1 serves as an important scaffold protein in cellular signaling. Weighing around 87 kDa KSR1 localizes in both the cytoplasm and the membrane of cells and shows expression in various tissues including the brain lung and ovaries. KSR1 does not have kinase activity itself but influences the activity of other kinases through its interactions.
- Biological function summary
KSR1 exerts a regulatory role by organizing multi-protein complexes necessary for effective Ras and Raf signaling. This protein helps assemble the mitogen-activated protein kinase (MAPK) cascade complex which plays an essential role in cell growth and differentiation. As an important scaffolding component KSR1 enhances the efficiency of signal transduction pathways by facilitating the spatial organization of kinases involved in MAPK/ERK signaling.
- Pathways
Signaling through KSR1 significantly impacts the MAPK/ERK and Ras pathways both of which are important for transmitting extracellular signals to the nucleus. KSR1 interacts with c-Raf MEK1/2 and ERK1/2 within these pathways forming a bridge that allows effective transduction of signals necessary for various cellular responses. By providing a platform for these kinases KSR1 ensures precise regulation of the signaling processes that control cell proliferation and survival.
- Associated diseases and disorders
Dysfunction in KSR1 activity associates with cancer and neurodegenerative disorders. KSR1 aberrations can contribute to oncogenic Ras-driven cancers due to its central role in controlling the Ras signaling pathway. Furthermore irregular KSR1 expression also links to neurodegenerative diseases through its impact on MAPK/ERK signaling. Proteins such as c-Raf and ERK1/2 which interact with KSR1 also play roles in these pathologies emphasizing the potential of KSR1 as a target for therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-KSR1 antibody [EPR2421Y] - BSA and Azide free (ab227109), expandable thumbnail](https://content.abcam.com/products/images/ksr1-antibody-epr2421y-bsa-and-azide-free-ab227109--western-blot-img143788.jpg "Western blot - Anti-KSR1 antibody [EPR2421Y] - BSA and Azide free (ab227109)")
Western blot - Anti-KSR1 antibody [EPR2421Y] - BSA and Azide free (ab227109)
This WB data was generated using the same anti-KSR1 antibody clone, EPR2421Y, in a different buffer formulation (cat# Anti-KSR1 antibody [EPR2421Y] ab68483).
Lane 1: Wild-type HAP1 cell lysate (40 μg)
Lane 2: KSR1 knockout HAP1 cell lysate (40 μg)
Lane 3: HeLa cell lysate (20 μg)
Lane 4: HEK293 cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - Anti-KSR1 antibody [EPR2421Y] ab68483 observed at 120 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.Unpurified Anti-KSR1 antibody [EPR2421Y] ab68483 was shown to recognize KSR1 when KSR1 knockout samples were used, along with additional cross-reactive bands. Wild-type and KSR1 knockout samples were subjected to SDS-PAGE. Anti-KSR1 antibody [EPR2421Y] ab68483 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-KSR1 antibody [EPR2421Y] (Anti-KSR1 antibody [EPR2421Y] ab68483)
Predicted band size: 102 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KSR1 antibody [EPR2421Y] - BSA and Azide free (ab227109), expandable thumbnail](https://content.abcam.com/products/images/ksr1-antibody-epr2421y-bsa-and-azide-free-ab227109--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img45527.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KSR1 antibody [EPR2421Y] - BSA and Azide free (ab227109)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KSR1 antibody [EPR2421Y] - BSA and Azide free (ab227109)
This IHC data was generated using the same anti-KSR1 antibody clone, EPR2421Y, in a different buffer formulation (cat# Anti-KSR1 antibody [EPR2421Y] ab68483).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue sections labeling KSR1 with Purified Anti-KSR1 antibody [EPR2421Y] ab68483 at 1:100 dilution (1.49 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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