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AB129111

Anti-KSR1 (phospho S392) antibody [EPR2359(2)]

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(1 Publication)

Rabbit Recombinant Monoclonal KSR1 phospho S392 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KSR, KSR1, Kinase suppressor of Ras 1

3 Images
Western blot - Anti-KSR1 (phospho S392) antibody [EPR2359(2)] (AB129111)
  • WB

Lab

Western blot - Anti-KSR1 (phospho S392) antibody [EPR2359(2)] (AB129111)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-KSR1 (phospho S392) antibody [EPR2359(2)] (ab129111) at 1/1000 dilution

Lane 1:

Untreated HEK293 cell lysate at 10 µg

Lane 2:

HEK293 cell lysate treated with Calcyculin A at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 102 kDa

Observed band size: 110 kDa

false

Western blot - Anti-KSR1 (phospho S392) antibody [EPR2359(2)] (AB129111)
  • WB

Unknown

Western blot - Anti-KSR1 (phospho S392) antibody [EPR2359(2)] (AB129111)

All lanes:

Western blot - Anti-KSR1 (phospho S392) antibody [EPR2359(2)] (ab129111) at 1/1000 dilution

Lane 1:

293T cell lysate, untreated at 10 µg

Lane 2:

293T cell lysate treated with Calyculin A at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 102 kDa

false

OI-RD Scanning - Anti-KSR1 (phospho S392) antibody [EPR2359(2)] (AB129111)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-KSR1 (phospho S392) antibody [EPR2359(2)] (AB129111)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2359(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KSR1 also known as Kinase Suppressor of Ras 1 serves as an important scaffold protein in cellular signaling. Weighing around 87 kDa KSR1 localizes in both the cytoplasm and the membrane of cells and shows expression in various tissues including the brain lung and ovaries. KSR1 does not have kinase activity itself but influences the activity of other kinases through its interactions.
Biological function summary

KSR1 exerts a regulatory role by organizing multi-protein complexes necessary for effective Ras and Raf signaling. This protein helps assemble the mitogen-activated protein kinase (MAPK) cascade complex which plays an essential role in cell growth and differentiation. As an important scaffolding component KSR1 enhances the efficiency of signal transduction pathways by facilitating the spatial organization of kinases involved in MAPK/ERK signaling.

Pathways

Signaling through KSR1 significantly impacts the MAPK/ERK and Ras pathways both of which are important for transmitting extracellular signals to the nucleus. KSR1 interacts with c-Raf MEK1/2 and ERK1/2 within these pathways forming a bridge that allows effective transduction of signals necessary for various cellular responses. By providing a platform for these kinases KSR1 ensures precise regulation of the signaling processes that control cell proliferation and survival.

Dysfunction in KSR1 activity associates with cancer and neurodegenerative disorders. KSR1 aberrations can contribute to oncogenic Ras-driven cancers due to its central role in controlling the Ras signaling pathway. Furthermore irregular KSR1 expression also links to neurodegenerative diseases through its impact on MAPK/ERK signaling. Proteins such as c-Raf and ERK1/2 which interact with KSR1 also play roles in these pathologies emphasizing the potential of KSR1 as a target for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of a multiprotein signaling complex which promotes phosphorylation of Raf family members and activation of downstream MAP kinases (By similarity). Independently of its kinase activity, acts as MAP2K1/MEK1 and MAP2K2/MEK2-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1 or MAP2K2/MEK2, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 and/or MAP2K2/MEK2 (PubMed : 29433126). Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP (By similarity). Its kinase activity is unsure (By similarity). Some protein kinase activity has been detected in vitro, however the physiological relevance of this activity is unknown (By similarity).
See full target information KSR1 pS392
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 13:946637 PubMed36199693

2022

EPM2A acts as a protective factor in prostate cancer, evidence from a real-world patient cohort.

Applications

Unspecified application

Species

Unspecified reactive species

Qintao Ge,Jiawei Li,Junyue Tao,Rui Gao,Chen Jin,Jun Zhou,Meng Zhang,Zongyao Hao,Jialin Meng,Chaozhao Liang
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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