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AB278551

Anti-KSR1 (phospho S392) antibody [KSR1S392-3A4]

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Rabbit Recombinant Monoclonal KSR1 phospho S392 antibody. Suitable for Flow Cyt and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human KSR1 phospho S392.

View Alternative Names

KSR, KSR1, Kinase suppressor of Ras 1

3 Images
Flow Cytometry - Anti-KSR1 (phospho S392) antibody [KSR1S392-3A4] (AB278551)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-KSR1 (phospho S392) antibody [KSR1S392-3A4] (AB278551)

Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells secondary antibody only negative control (blue) treated with imatinib (red) treated with PV (green) treated with PV + blocked with non-phospho peptide (violet) or treated with PV + blocked with phospho-peptide (brown) using ab278551 at 0.05 μg/ml.

Flow Cytometry - Anti-KSR1 (phospho S392) antibody [KSR1S392-3A4] (AB278551)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-KSR1 (phospho S392) antibody [KSR1S392-3A4] (AB278551)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells untreated and unstained as negative control (blue) or treated with lambda phosphatase and stained (red) or untreated and stained (green) using ab278551 at 0.1 μg/ml.

Flow Cytometry - Anti-KSR1 (phospho S392) antibody [KSR1S392-3A4] (AB278551)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-KSR1 (phospho S392) antibody [KSR1S392-3A4] (AB278551)

Flow cytometric analysis of NIH/3T3 (mouse embryo fibroblast cell line) cells secondary antibody only (blue) or 0.1 μg/ml of isotype control (orange) or ab278551 (green).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

KSR1S392-3A4

Isotype

IgG

Light chain type

kappa

Carrier free

No

Reacts with

Human, Mouse

Applications

Flow Cyt

applications

Immunogen

Synthetic Peptide within Human KSR1 phospho S392. The exact immunogen used to generate this antibody is proprietary information.

Q8IVT5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KSR1 also known as Kinase Suppressor of Ras 1 serves as an important scaffold protein in cellular signaling. Weighing around 87 kDa KSR1 localizes in both the cytoplasm and the membrane of cells and shows expression in various tissues including the brain lung and ovaries. KSR1 does not have kinase activity itself but influences the activity of other kinases through its interactions.
Biological function summary

KSR1 exerts a regulatory role by organizing multi-protein complexes necessary for effective Ras and Raf signaling. This protein helps assemble the mitogen-activated protein kinase (MAPK) cascade complex which plays an essential role in cell growth and differentiation. As an important scaffolding component KSR1 enhances the efficiency of signal transduction pathways by facilitating the spatial organization of kinases involved in MAPK/ERK signaling.

Pathways

Signaling through KSR1 significantly impacts the MAPK/ERK and Ras pathways both of which are important for transmitting extracellular signals to the nucleus. KSR1 interacts with c-Raf MEK1/2 and ERK1/2 within these pathways forming a bridge that allows effective transduction of signals necessary for various cellular responses. By providing a platform for these kinases KSR1 ensures precise regulation of the signaling processes that control cell proliferation and survival.

Dysfunction in KSR1 activity associates with cancer and neurodegenerative disorders. KSR1 aberrations can contribute to oncogenic Ras-driven cancers due to its central role in controlling the Ras signaling pathway. Furthermore irregular KSR1 expression also links to neurodegenerative diseases through its impact on MAPK/ERK signaling. Proteins such as c-Raf and ERK1/2 which interact with KSR1 also play roles in these pathologies emphasizing the potential of KSR1 as a target for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of a multiprotein signaling complex which promotes phosphorylation of Raf family members and activation of downstream MAP kinases (By similarity). Independently of its kinase activity, acts as MAP2K1/MEK1 and MAP2K2/MEK2-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1 or MAP2K2/MEK2, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 and/or MAP2K2/MEK2 (PubMed : 29433126). Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP (By similarity). Its kinase activity is unsure (By similarity). Some protein kinase activity has been detected in vitro, however the physiological relevance of this activity is unknown (By similarity).
See full target information KSR1 phospho S392
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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