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AB173377

Anti-KSR2 antibody

4

(1 Review)

|

(2 Publications)

Mouse Polyclonal KSR2 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Kinase suppressor of Ras 2 aa 100 to C-terminus.

View Alternative Names

Kinase suppressor of Ras 2, hKSR2, KSR2

1 Images
Western blot - Anti-KSR2 antibody (AB173377)
  • WB

Supplier Data

Western blot - Anti-KSR2 antibody (AB173377)

All lanes:

Western blot - Anti-KSR2 antibody (ab173377) at 1 µg/mL

Lane 1:

KSR2-transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Predicted band size: 108 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human Kinase suppressor of Ras 2 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6VAB6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The KSR2 protein also known as Kinase Suppressor of Ras 2 operates as a scaffold protein. It functions by assembling a protein complex that is important for signal transduction. KSR2 has a molecular weight of approximately 104 kDa. This protein is widely expressed in various tissues most notably in the brain and adipose tissue where it plays key roles in cellular communication and metabolic regulation.
Biological function summary

Kinase Suppressor of Ras 2 participates in the scaffolding of protein complexes required for MEK/ERK signaling. KSR2 contributes to the regulation of cellular energy homeostasis and metabolic processes. It also acts as a facilitator that enhances the signaling efficiency of the MAPK pathway allowing proper transduction of growth and developmental signals. Its role in the modulation of energy balance involves effects on both glucose and fatty acid metabolism.

Pathways

KSR2 interacts notably with the MEK/ERK and AMPK signaling pathways. KSR2 modulates the MAPK pathway facilitating the phosphorylation of ERK an essential step for transmitting signals from membrane receptors to the nucleus. In the AMPK pathway KSR2 interacts indirectly with AMPK-related proteins impacting energy balance and cellular stress responses. Through these pathways KSR2 links with key proteins like Raf MEK and LKB1 important for signal transduction and energy regulation.

KSR2 plays a significant role in obesity and type 2 diabetes. Mutations in KSR2 can lead to severe obesity accompanied by insulin resistance indicating its importance in regulating metabolism and energy consumption. Through these disorders KSR2 links with proteins such as AMPK which influences energy homeostasis and insulin signaling. Addressing KSR2 dysfunction may have therapeutic potential for metabolic diseases highlighting its importance in health and disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Location-regulated scaffold connecting MEK to RAF. Has very low protein kinase activity and can phosphorylate MAP2K1 at several Ser and Thr residues with very low efficiency (in vitro). Acts as MAP2K1/MEK1-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 (PubMed : 29433126). Interaction with BRAF enhances KSR2-mediated phosphorylation of MAP2K1 (in vitro). Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.
See full target information Kinase suppressor of Ras 2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 27:101560 PubMed36252281

2022

Chloride intracellular channel 1 promotes esophageal squamous cell carcinoma proliferation via mTOR signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Huiwu Geng,Cheng Feng,Zhangran Sun,Xu Fan,Yiqing Xie,Jinghua Gu,Libin Fan,Gang Liu,Chao Li,Rick F Thorne,Xu Dong Zhang,Xinying Li,Xiaoying Liu

Communications biology 5:780 PubMed35918402

2022

Targeted inhibition of ubiquitin signaling reverses metabolic reprogramming and suppresses glioblastoma growth.

Applications

Unspecified application

Species

Unspecified reactive species

Rossella Delle Donne,Rosa Iannucci,Laura Rinaldi,Luca Roberto,Maria A Oliva,Emanuela Senatore,Domenica Borzacchiello,Luca Lignitto,Giorgio Giurato,Francesca Rizzo,Assunta Sellitto,Francesco Chiuso,Salvatore Castaldo,Giovanni Scala,Virginia Campani,Valeria Nele,Giuseppe De Rosa,Chiara D'Ambrosio,Corrado Garbi,Andrea Scaloni,Alessandro Weisz,Concetta Ambrosino,Antonella Arcella,Antonio Feliciello
View all publications
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