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AB202022

Anti-Ku70 antibody [2F7F5]

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(5 Publications)

Mouse Monoclonal Ku70 antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human, Rat, Transfected cell lysate - Human, Recombinant fragment samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human XRCC6 aa 1-250.

View Alternative Names

G22P1, XRCC6, DNA repair protein Ku70, 5'-deoxyribose-5-phosphate lyase Ku70, 70 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, CTC box-binding factor 75 kDa subunit, DNA repair protein XRCC6, Lupus Ku autoantigen protein p70, Thyroid-lupus autoantigen, X-ray repair complementing defective repair in Chinese hamster cells 6, X-ray repair cross-complementing protein 6, 5'-dRP lyase Ku70, CTC75, CTCBF, Ku70, TLAA

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku70 antibody [2F7F5] (AB202022)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku70 antibody [2F7F5] (AB202022)

Immunohistochemical analysis of paraffin embedded Human endometrial cancer tissues labeling Ku70 using ab202022 at 1/200 dilution, followed by DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku70 antibody [2F7F5] (AB202022)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku70 antibody [2F7F5] (AB202022)

Immunohistochemical analysis of paraffin embedded Human cervical cancer tissues labeling Ku70 using ab202022 at 1/200 dilution, followed by DAB staining.

Flow Cytometry - Anti-Ku70 antibody [2F7F5] (AB202022)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Ku70 antibody [2F7F5] (AB202022)

Flow cytometric analysis of A431 cells labeling Ku70 using ab202022 (green) at 1/200 dilution, and negative control (red).

Western blot - Anti-Ku70 antibody [2F7F5] (AB202022)
  • WB

Supplier Data

Western blot - Anti-Ku70 antibody [2F7F5] (AB202022)

All lanes:

Western blot - Anti-Ku70 antibody [2F7F5] (ab202022) at 1/500 dilution

Lane 1:

HeLa cell lysate at 1 µg

Lane 2:

PC-2 cell lysate at 1 µg

Lane 3:

A549 cell lysate at 1 µg

Lane 4:

A431 cell lysate at 1 µg

Lane 5:

HepG2 cell lysate at 1 µg

Lane 6:

K562 cell lysate at 1 µg

Predicted band size: 70 kDa

false

Western blot - Anti-Ku70 antibody [2F7F5] (AB202022)
  • WB

Supplier Data

Western blot - Anti-Ku70 antibody [2F7F5] (AB202022)

All lanes:

Western blot - Anti-Ku70 antibody [2F7F5] (ab202022) at 1/500 dilution

Lane 1:

Non transfected HEK293 cell lysate at 1 µg

Lane 2:

Ku70 (aa: 6-214)-hIgGFc transfected HEK293 cell lysate at 1 µg

Predicted band size: 70 kDa

false

Western blot - Anti-Ku70 antibody [2F7F5] (AB202022)
  • WB

Supplier Data

Western blot - Anti-Ku70 antibody [2F7F5] (AB202022)

Expected MW is 49.7 kDa

All lanes:

Western blot - Anti-Ku70 antibody [2F7F5] (ab202022) at 1/500 dilution

All lanes:

Ku70 (aa: 6-214) recombinant protein. at 1 µg

Predicted band size: 70 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2F7F5

Isotype

IgG1

Carrier free

No

Reacts with

Rat, Human

Applications

Flow Cyt, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human XRCC6 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P12956

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant fragment": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell lysate - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ku70 also known as XRCC6 or K331 is a DNA repair protein with a molecular mass of approximately 70 kDa. It belongs to the Ku protein family which includes Ku80. This protein expresses mainly in the nucleus of cells across various tissue types. As a part of a heterodimer together with Ku80 Ku70 forms the Ku70/Ku80 complex which binds to free DNA ends allowing DNA repair processes to occur efficiently.
Biological function summary

The Ku70 protein plays a significant role in maintaining genomic stability. It is an important component of the non-homologous end joining (NHEJ) pathway a primary mechanism for repairing double-strand breaks in DNA. By forming the Ku70/Ku80 complex it accurately aligns DNA ends to facilitate repair and prevent chromosomal abnormalities. The Ku70 protein also involves in telomere maintenance and can influence cell cycle regulation.

Pathways

Ku70 contributes mainly to the NHEJ DNA repair pathway. This pathway is important for cellular response to DNA damage and involves other proteins such as DNA-PKcs (DNA-dependent protein kinase catalytic subunit) and XRCC4. Ku70's interaction with DNA-PKcs enables the activation of its kinase activity which is essential for the repair process. Additionally Ku70 is involved in the homologous recombination pathway where it interacts with proteins like Rad51 to ensure repair fidelity.

Research links the Ku70 protein to cancer and neurodegenerative diseases. Dysregulation or mutations affecting Ku70 can lead to genomic instability contributing to cancer development. Ku70 also shows connections to neurodegenerative conditions like Alzheimer's disease where its role in DNA repair and apoptosis regulation may impact neuronal cell survival. Interactions with p53 a protein important for DNA damage response further underline Ku70's influence in these disease contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

DNA-binding protein critical for the DNA damage response, specifically in repairing double-strand breaks (DSBs) via the classical non-homologous end joining (NHEJ) pathway. It forms a heterodimer with XRCC5 (Ku80), creating the Ku70 : Ku80 heterodimer (Ku complex), which serves as a DNA end-binding complex. It primarily binds DSBs and recruits essential repair factors, assembling the core long-range NHEJ complex to facilitate the alignment and ligation of broken DNA ends (PubMed : 11493912, PubMed : 20493174, PubMed : 33854234, PubMed : 34352203, PubMed : 9742108). This pathway ensures the rapid repair of cytotoxic and mutagenic DSBs and contributes to the generation of diversity in T-cell receptors and antibodies through mechanisms such as V(D)J recombination (PubMed : 9742108). Likely acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), catalyzing the beta-elimination of the 5'-deoxyribose-5-phosphate at abasic sites near DSBs. This activity cleans the termini of abasic sites, a common form of nucleotide damage, preparing broken ends for ligation (PubMed : 20383123). It may also possess 3'-5' DNA helicase activity, although this has not been confirmed in vivo, and its physiological significance remains unclear (PubMed : 7957065). Beyond DNA repair, the protein contributes to telomere maintenance (PubMed : 29490055). It is also implicated in transcriptional regulation, acting as a cofactor for various transcription factors (PubMed : 12145306, PubMed : 8621488). It plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed : 28712728). Can also bind RNAs and recruits PRKDC to a wide range of cellular RNAs, including the U3 small nucleolar RNA, playing a role in the biogenesis of ribosomal RNAs (PubMed : 32103174). Additionally, it negatively regulates apoptosis by interacting with BAX, sequestering it from the mitochondria, and may possess deubiquitination activity targeting BAX (PubMed : 15023334, PubMed : 18362350, PubMed : 35545041).
See full target information XRCC6
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Experimental hematology & oncology 13:109 PubMed39497152

2024

Nuclear porcupine mediates XRCC6/Ku70 S-palmitoylation in the DNA damage response.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Chen,Mingming Xiao,Yaqi Mo,Jinlu Ma,Yamei Han,Qing Li,Qinghua Zeng,Rebecca J Boohaker,Joshua Fried,Yonghe Li,Han Wang,Bo Xu

Cancer biology & therapy 25:2402588 PubMed39286953

2024

EX527, a sirtuins 1 inhibitor, sensitizes T-cell leukemia to death receptor-mediated apoptosis by downregulating cellular FLICE inhibitory protein.

Applications

Unspecified application

Species

Unspecified reactive species

Rongqi Guo,Yihui Wei,Yating Du,Luyue Liu,Haoqi Zhang,Ruiying Ren,Ruili Sun,Tingting Zhang,Xiwen Xiong,Lijun Zhao,Hongfei Wang,Xiaofang Guo,Xiaofei Zhu

Nature communications 13:1825 PubMed35383176

2022

Structure-based design of stabilized recombinant influenza neuraminidase tetramers.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Ellis,Julia Lederhofer,Oliver J Acton,Yaroslav Tsybovsky,Sally Kephart,Christina Yap,Rebecca A Gillespie,Adrian Creanga,Audrey Olshefsky,Tyler Stephens,Deleah Pettie,Michael Murphy,Claire Sydeman,Maggie Ahlrichs,Sidney Chan,Andrew J Borst,Young-Jun Park,Kelly K Lee,Barney S Graham,David Veesler,Neil P King,Masaru Kanekiyo

Frontiers in oncology 11:689063 PubMed34222016

2021

A Fibrinogen Alpha Fragment Mitigates Chemotherapy-Induced Rearrangements.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Eberle,Rahel Stefanie Wiehe,Boris Gole,Liska Jule Mattis,Anja Palmer,Ludger Ständker,Wolf-Georg Forssmann,Jan Münch,J Christof M Gebhardt,Lisa Wiesmüller

Nature communications 11:3531 PubMed32669601

2020

Sequential role of RAD51 paralog complexes in replication fork remodeling and restart.

Applications

Unspecified application

Species

Unspecified reactive species

Matteo Berti,Federico Teloni,Sofija Mijic,Sebastian Ursich,Jevgenij Fuchs,Maria Dilia Palumbieri,Jana Krietsch,Jonas A Schmid,Edwige B Garcin,Stéphanie Gon,Mauro Modesti,Matthias Altmeyer,Massimo Lopes
View all publications
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Product promise

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