Rabbit Polyclonal Ku80 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human XRCC6.
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- Cancer medicine 11:3809-38192022RAD18 confers radioresistance of esophagus squamous cell carcinoma through regulating p-DNA-PKcs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoqing Li,Shitao Zou,Liangsu Zhou,Aidi Gao,Jing Xu,Chao He,Jundong Zhou,Shuhua Wu,Yihong ChenPubMed 35426246
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human XRCC6. The exact immunogen used to generate this antibody is proprietary information. Database link P12956
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Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
Associated Products
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Target data
Function
Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA (PubMed:11493912, PubMed:12145306, PubMed:7957065, PubMed:8621488). Required for double-strand break repair and V(D)J recombination (PubMed:11493912, PubMed:12145306, PubMed:7957065, PubMed:8621488). Also has a role in chromosome translocation (PubMed:11493912, PubMed:12145306, PubMed:7957065, PubMed:8621488). The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner (PubMed:11493912, PubMed:12145306, PubMed:7957065, PubMed:8621488). It works in the 3'-5' direction (PubMed:11493912, PubMed:12145306, PubMed:7957065, PubMed:8621488). During NHEJ, the XRCC5-XRRC6 dimer performs the recognition step: it recognizes and binds to the broken ends of the DNA and protects them from further resection (PubMed:11493912, PubMed:12145306, PubMed:7957065, PubMed:8621488). Binding to DNA may be mediated by XRCC6 (PubMed:11493912, PubMed:12145306, PubMed:7957065, PubMed:8621488). The XRCC5-XRRC6 dimer acts as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold (PubMed:11493912, PubMed:12145306, PubMed:20383123, PubMed:7957065, PubMed:8621488). The XRCC5-XRRC6 dimer is probably involved in stabilizing broken DNA ends and bringing them together (PubMed:12145306, PubMed:20383123, PubMed:7957065, PubMed:8621488). The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step (PubMed:12145306, PubMed:20383123, PubMed:7957065, PubMed:8621488). The XRCC5-XRRC6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks (PubMed:20383123). XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined (PubMed:20383123). The XRCC5-XRRC6 dimer together with APEX1 acts as a negative regulator of transcription (PubMed:8621488). In association with NAA15, the XRCC5-XRRC6 dimer binds to the osteocalcin promoter and activates osteocalcin expression (PubMed:12145306). As part of the DNA-PK complex, involved in the early steps of ribosome assembly by promoting the processing of precursor rRNA into mature 18S rRNA in the small-subunit processome (PubMed:32103174). Binding to U3 small nucleolar RNA, recruits PRKDC and XRCC5/Ku86 to the small-subunit processome (PubMed:32103174). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed:28712728).
Additional Targets
XRCC6
Alternative names
G22P2, XRCC5, X-ray repair cross-complementing protein 5, 86 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit, CTC box-binding factor 85 kDa subunit, DNA repair protein XRCC5, Ku80, Ku86, Lupus Ku autoantigen protein p86, Nuclear factor IV, Thyroid-lupus autoantigen, X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining), CTC85, CTCBF, TLAA
Recommended products
Rabbit Polyclonal Ku80 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human XRCC6.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human XRCC6. The exact immunogen used to generate this antibody is proprietary information. Database link P12956
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ku70 and Ku80 also known collectively as the Ku70/Ku80 complex are proteins that play a vital role in DNA repair. Ku70 and Ku80 are subunits of a heterodimeric protein with Ku70 approximately 70 kDa and Ku80 around 80 kDa in mass. These proteins are typically expressed in the nucleus of eukaryotic cells where they recognize and bind to DNA double-strand break ends. The Ku70/Ku80 complex serves as a scaffold for the assembly of other proteins involved in non-homologous end joining (NHEJ) an important pathway for repairing these DNA breaks.
- Biological function summary
Ku70 and Ku80 form a ring-shaped structure that binds tightly around DNA ends facilitating high-fidelity repair processes. They are essential for maintaining genomic stability by ensuring accurate repair of DNA that can otherwise lead to chromosomal aberrations. Ku70/80 operates as part of the DNA-PK complex working alongside DNA-PKcs (DNA-dependent protein kinase catalytic subunit) to promote the NHEJ pathway. This complex orchestrates the repair process by protecting DNA ends and recruiting other essential repair factors.
- Pathways
NHEJ and V(D)J recombination are two critical biological pathways where Ku70/Ku80 functions. NHEJ is a major pathway for repairing double-strand breaks especially in the context of DNA damage by radiation or spontaneous genomic instability. V(D)J recombination on the other hand is essential for generating diversity in antigen receptors during immune response. Within these pathways the Ku70/Ku80 complex works closely with DNA ligase IV and XRCC4 to facilitate the final ligation step thereby ensuring effective DNA repair and recombination.
- Associated diseases and disorders
The malfunction of Ku70/Ku80 can lead to severe immunodeficiency disorders and increase the risk of cancer. Deficiencies in the Ku70/Ku80 complex result in a compromised NHEJ repair mechanism leading to increased susceptibility to genomic instability and associated malignancies. Additionally the improper function of Ku70/Ku80 is linked to neurodegenerative diseases due to failed DNA repair also involving other proteins like PARP1 (Poly [ADP-ribose] polymerase 1) highlighting the importance of the complex in maintaining neural health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-Ku70 + Ku80 antibody - C-terminal (ab166822)
All lanes: Western blot - Anti-Ku70 + Ku80 antibody - C-terminal (ab166822) at 1/500 dilution
Lane 1: LoVo cell lysate
Lane 2: LoVo cell lysate with synthesized peptide
Predicted band size: 70 kDa, 82 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku70 + Ku80 antibody - C-terminal (ab166822)
Immunohistochemical analysis of formalin fixed, paraffin embedded Human kidney tissue labeling Ku70 + Ku80 with ab166822 at 5 µg/ml.
Immunocytochemistry/ Immunofluorescence - Anti-Ku70 + Ku80 antibody - C-terminal (ab166822)
Immunofluorescence analysis of A549 cells labeling Ku70 + Ku80 with ab166822 at 1/100. The panel on the right is treated with the synthesized peptide.
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