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AB80592

Anti-Ku80 antibody [EPR3468]

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(54 Publications)

Anti-Ku80 antibody [EPR3468] (ab80592) is a rabbit monoclonal antibody detecting Ku80 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 40 publications
- Trusted since 2009

View Alternative Names

G22P2, XRCC5, X-ray repair cross-complementing protein 5, 86 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit, CTC box-binding factor 85 kDa subunit, DNA repair protein XRCC5, Ku80, Ku86, Lupus Ku autoantigen protein p86, Nuclear factor IV, Thyroid-lupus autoantigen, X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining), CTC85, CTCBF, TLAA

4 Images
Flow Cytometry (Intracellular) - Anti-Ku80 antibody [EPR3468] (AB80592)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Ku80 antibody [EPR3468] (AB80592)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Ku80 with unpurified ab80592 at 1/20 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

Immunoprecipitation - Anti-Ku80 antibody [EPR3468] (AB80592)
  • IP

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Immunoprecipitation - Anti-Ku80 antibody [EPR3468] (AB80592)

ab80592 (purified) at 1/500 dilution (1.86 © : g/ml) immunoprecipitating Ku80 in HeLa whole cell lysate.
Lane 1 (input) : HeLa(Human cervix adenocarcinoma epithelial cell) whole cell lysate 10© : g
Lane 2 (+) : ab80592 & HeLa whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab80592 in HeLa whole cell lysate
For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1/1000 dilution.
Blocking and diluting buffer : 5% NFDM /TBST .

All lanes:

Immunoprecipitation - Anti-Ku80 antibody [EPR3468] (ab80592)

Predicted band size: 83 kDa

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Western blot - Anti-Ku80 antibody [EPR3468] (AB80592)
  • WB

Lab

Western blot - Anti-Ku80 antibody [EPR3468] (AB80592)

All lanes:

Western blot - Anti-Ku80 antibody [EPR3468] (ab80592) at 1/3000 dilution

Lane 1:

PC-12(Rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Lane 2:

NIH/3T3(Mouse embryonic fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 83 kDa

Observed band size: 83 kDa

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Western blot - Anti-Ku80 antibody [EPR3468] (AB80592)
  • WB

Unknown

Western blot - Anti-Ku80 antibody [EPR3468] (AB80592)

All lanes:

Western blot - Anti-Ku80 antibody [EPR3468] (ab80592) at 1/50000 dilution

Lane 1:

A549 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

MCF7 cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 83 kDa

Observed band size: 83 kDa

false

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Ku80 antibody [EPR3468]

  • Carrier free

    Anti-Ku80 antibody [EPR3468] - BSA and Azide free

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Ku80 antibody [EPR3468]

  • HRP

    HRP Anti-Ku80 antibody [EPR3468]

  • 578 PE

    PE Anti-Ku80 antibody [EPR3468]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3468

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, Flow Cyt (Intra), WB, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p>The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p>The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p>The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-Ku80 antibody [EPR3468] (ab80592) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of Ku80?
Anti-Ku80 [EPR3468] (ab80592) specifically detects a band for Ku80 (UniProt: P13010) at a molecular weight of 83kDa.

Trusted by the scientific community
Anti-Ku80 [EPR3468] (ab80592) was first used in a scientific publication in 2009 and has been cited over 40 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR3468] also available for your convenience: ab80592, Alexa Fluor® 488 - ab198586, Alexa Fluor® 647 - ab198587, HRP - ab199096, PE - ab225046, Carrier free - ab232381

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Ku80 protein also known as Ku p70 Ku antigen p70 and by gene identifiers such as Ku80 hum39 and 5c5 functions critically in DNA repair processes. With a molecular mass of approximately 80 kDa Ku80 is ubiquitously expressed in mammalian cells. It is an important component of the Ku heterodimer working with its partner Ku70 to detect and bind DNA double-strand breaks (DSBs). This action initiates repair by aligning with DNA ends to prevent break processing and sustain genome integrity.
Biological function summary

The Ku80 protein executes an important role in maintaining cellular stability by participating in the non-homologous end joining (NHEJ) pathway. Ku80 forms a complex with DNA-PKcs (DNA-dependent protein kinase catalytic subunit) which activates the kinase to phosphorylate itself and other proteins facilitating the repair of DSBs. Its expression is significant in tissues that undergo high rates of cellular turnover and are experienced with DNA damage where the DNA repair mechanisms are important for regular cell cycle progression.

Pathways

DNA repair and V(D)J recombination are principal domains where Ku80 shows its influence. In the context of DNA repair Ku80's partnership with DNA-PKcs as part of the NHEJ repair pathway is fundamental in fixing DNA damage from environmental insults or normal cellular metabolism. In the immune development process through V(D)J recombination Ku80 partners with other proteins like the Artemis complex to perform programmed DNA rearrangements required for the diversity of immune receptors.

Ku80 has a significant relation to cancer and immune deficiencies. Defects in Ku80 function can lead to increased sensitivity to ionizing radiation and a higher propensity for cancer development because of impaired DNA repair capabilities. Moreover in immune-related disorders inadequate Ku80 performance affects the maturation of lymphocytes leading to immune system malfunction. Its malfunctioning connection with proteins like DNA-PKcs and Artemis highlights its critical roles in both tumor suppression and immune system competence.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA (PubMed : 11493912, PubMed : 12145306, PubMed : 7957065, PubMed : 8621488). Required for double-strand break repair and V(D)J recombination (PubMed : 11493912, PubMed : 12145306, PubMed : 7957065, PubMed : 8621488). Also has a role in chromosome translocation (PubMed : 11493912, PubMed : 12145306, PubMed : 7957065, PubMed : 8621488). The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner (PubMed : 11493912, PubMed : 12145306, PubMed : 7957065, PubMed : 8621488). It works in the 3'-5' direction (PubMed : 11493912, PubMed : 12145306, PubMed : 7957065, PubMed : 8621488). During NHEJ, the XRCC5-XRRC6 dimer performs the recognition step : it recognizes and binds to the broken ends of the DNA and protects them from further resection (PubMed : 11493912, PubMed : 12145306, PubMed : 7957065, PubMed : 8621488). Binding to DNA may be mediated by XRCC6 (PubMed : 11493912, PubMed : 12145306, PubMed : 7957065, PubMed : 8621488). The XRCC5-XRRC6 dimer acts as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold (PubMed : 11493912, PubMed : 12145306, PubMed : 20383123, PubMed : 7957065, PubMed : 8621488). The XRCC5-XRRC6 dimer is probably involved in stabilizing broken DNA ends and bringing them together (PubMed : 12145306, PubMed : 20383123, PubMed : 7957065, PubMed : 8621488). The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step (PubMed : 12145306, PubMed : 20383123, PubMed : 7957065, PubMed : 8621488). The XRCC5-XRRC6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks (PubMed : 20383123). XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined (PubMed : 20383123). The XRCC5-XRRC6 dimer together with APEX1 acts as a negative regulator of transcription (PubMed : 8621488). In association with NAA15, the XRCC5-XRRC6 dimer binds to the osteocalcin promoter and activates osteocalcin expression (PubMed : 12145306). As part of the DNA-PK complex, involved in the early steps of ribosome assembly by promoting the processing of precursor rRNA into mature 18S rRNA in the small-subunit processome (PubMed : 32103174). Binding to U3 small nucleolar RNA, recruits PRKDC and XRCC5/Ku86 to the small-subunit processome (PubMed : 32103174). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed : 28712728).
See full target information XRCC5
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Publications (54)

Recent publications for all applications. Explore the full list and refine your search

Stem cell reviews and reports : PubMed41075148

2025

Transplantation of Human iPS Cell-derived Cerebral Cortical Neurons Promotes Fine Motor Recovery in a Female Mouse Model of Ischemic Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Hokuto Yamashita,Tetsuhiro Kikuchi,Yusaku Kodaka,Daisuke Doi,Megumi Ikeda,Jun Takahashi

Nature 646:992-1000 PubMed40903580

2025

DNA2 enables growth by restricting recombination-restarted replication.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica J R Hudson,Rowin Appanah,David Jones,Kathryn Davidson,Alice M Budden,Alina Vaitsiankova,Kok-Lung Chan,Keith W Caldecott,Antony M Carr,Ulrich Rass

Nature communications 16:7832 PubMed40846865

2025

Centromere protection requires strict mitotic inactivation of the Bloom syndrome helicase complex.

Applications

Unspecified application

Species

Unspecified reactive species

María Fernández-Casañas,Eleftheria Karanika,Umit Aliyaskarova,Tomisin Olukoga,Alex D Herbert,Antony W Oliver,Matthew Day,Adrijana Crncec,Kok-Lung Chan

Experimental hematology & oncology 13:109 PubMed39497152

2024

Nuclear porcupine mediates XRCC6/Ku70 S-palmitoylation in the DNA damage response.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Chen,Mingming Xiao,Yaqi Mo,Jinlu Ma,Yamei Han,Qing Li,Qinghua Zeng,Rebecca J Boohaker,Joshua Fried,Yonghe Li,Han Wang,Bo Xu

Nature communications 15:8208 PubMed39294166

2024

Germline variant affecting p53β isoforms predisposes to familial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie A Schubert,Dina Ruano,Sebastien M Joruiz,Jordy Stroosma,Nikolina Glavak,Anna Montali,Lia M Pinto,Mar Rodríguez-Girondo,Daniela Q C M Barge-Schaapveld,Maartje Nielsen,Bernadette P M van Nesselrooij,Arjen R Mensenkamp,Monique E van Leerdam,Thomas H Sharp,Hans Morreau,Jean-Christophe Bourdon,Noel F C C de Miranda,Tom van Wezel

Cell death & disease 15:649 PubMed39231972

2024

L3MBTL1, a polycomb protein, promotes Osimertinib acquired resistance through epigenetic regulation of DNA damage response in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zihe Zhang,Yongwen Li,Ruifeng Shi,Chaoyi Jia,Songlin Xu,Guangsheng Zhu,Peijun Cao,Hua Huang,Xuanguang Li,Hongbing Zhang,Minghui Liu,Chen Chen,Hongyu Liu,Chunsheng Kang,Jun Chen

Nature communications 15:6843 PubMed39122671

2024

Enhancing homology-directed repair efficiency with HDR-boosting modular ssDNA donor.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Ying Jin,Peng Zhang,Le-Le Liu,Xiang Zhao,Xiao-Qing Hu,Si-Zhe Liu,Ze-Kun Li,Qian Liu,Jian-Qiao Wang,De-Long Hao,Zhu-Qin Zhang,Hou-Zao Chen,De-Pei Liu

Neuron 112:2686-2707.e8 PubMed38897209

2024

Therapeutic potential of human microglia transplantation in a chimeric model of CSF1R-related leukoencephalopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Jean Paul Chadarevian,Jonathan Hasselmann,Alina Lahian,Joia K Capocchi,Adrian Escobar,Tau En Lim,Lauren Le,Christina Tu,Jasmine Nguyen,Sepideh Kiani Shabestari,William Carlen-Jones,Sunil Gandhi,Guojun Bu,David A Hume,Clare Pridans,Zbigniew K Wszolek,Robert C Spitale,Hayk Davtyan,Mathew Blurton-Jones

Cell stem cell 30:1331-1350.e11 PubMed37802038

2023

Human pallial MGE-type GABAergic interneuron cell therapy for chronic focal epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Marina Bershteyn,Sonja Bröer,Mansi Parekh,Yves Maury,Steven Havlicek,Sonja Kriks,Luis Fuentealba,Seonok Lee,Robin Zhou,Geetha Subramanyam,Meliz Sezan,Eric Steven Sevilla,Whitney Blankenberger,Julien Spatazza,Li Zhou,Hubert Nethercott,David Traver,Philip Hampel,Hannah Kim,Michael Watson,Naomi Salter,Anastasia Nesterova,Wai Au,Arnold Kriegstein,Arturo Alvarez-Buylla,John Rubenstein,Gautam Banik,Alessandro Bulfone,Catherine Priest,Cory R Nicholas

Human genetics 142:1417-1427 PubMed37558815

2023

Inactivating TDP2 missense mutation in siblings with congenital abnormalities reminiscent of fanconi anemia.

Applications

Unspecified application

Species

Unspecified reactive species

Guido Zagnoli-Vieira,Jan Brazina,Kris Van Den Bogaert,Wim Huybrechts,Guy Molenaers,Keith W Caldecott,Hilde Van Esch
View all publications
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