AB181173

Anti-kur antibody [EPR13320-66]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal kur antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C21orf48, C21orf59, CFAP298, Cilia- and flagella-associated protein 298, Protein kurly homolog

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-kur antibody [EPR13320-66] (AB181173)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-kur antibody [EPR13320-66] (AB181173)

Immunofluorescent analysis of MCF7 cells (paraformaldehyde-fixed 4%) labeling kur with ab181173 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Supplier Data

Immunoprecipitation - Anti-kur antibody [EPR13320-66] (AB181173)

Western blot analysis of Jurkat cell lysate immunoprecipitated using ab181173 at 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody used at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-kur antibody [EPR13320-66] (ab181173)

Predicted band size: 33 kDa

false

Supplier Data

Western blot - Anti-kur antibody [EPR13320-66] (AB181173)

All lanes:

Western blot - Anti-kur antibody [EPR13320-66] (ab181173) at 1/5000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 33 kDa

false

Carrier free

Anti-kur antibody [EPR13320-66] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13320-66

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40 - 1/60", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kur also known as the voltage-gated potassium channel subunit Kv3.x is an integral membrane protein involved in potassium ion transport across cell membranes. With a molecular mass of approximately 85 kDa this channel exhibits a high expression level in central nervous system tissues particularly in neurons. It plays a critical role in modulating neuronal excitability and signal transmission by facilitating rapid repolarization of the action potential.

Biological function summary

The voltage-gated potassium channel Kv3.x influences neurotransmitter release and neuronal firing rate. This target does not function alone; rather it forms a part of a larger channel complex. Through these channels Kv3.x regulates the rapid spike frequency adaptation enabling neurons to maintain high-frequency firing without depolarization block. By contributing to electrical signaling precision it supports diverse neuronal circuit activities.

Pathways

The potassium channel Kv3.x integrates into the neuronal excitability pathway affecting both synaptic transmission and plasticity. It interacts with key proteins like Nav1.6 a voltage-gated sodium channel as they complement each other's roles in action potential propagation. Kv3.x also participates in the broader potassium ion homeostasis pathway which involves other ion channels and transporters to maintain neuronal ion balance and function.

Dysregulation of Kv3.x channels associates with neurological conditions such as epilepsy and schizophrenia. Altered expression or function of Kv3.x can lead to increased neuronal excitability contributing to seizure susceptibility in epilepsy. In schizophrenia abnormal Kv3.x activity could disrupt normal auditory processing potentially through interactions with proteins like NMDA receptors. These associations highlight the importance of Kv3.x in maintaining neural health and signaling fidelity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in motile cilium function, possibly by acting on outer dynein arm assembly (PubMed : 24094744). Seems to be important for initiation rather than maintenance of cilium motility (By similarity). Required for correct positioning of the cilium at the apical cell surface, suggesting an additional role in the planar cell polarity (PCP) pathway (By similarity). May suppress canonical Wnt signaling activity (By similarity).

See full target information CFAP298

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 9: PubMed38912589

2024

Protein disulfide isomerase is essential for spermatogenesis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqiong Zhang,Aizhen Yang,Zhenzhen Zhao,Fengwu Chen,Xiaofeng Yan,Yue Han,Depei Wu,Yi Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-kur antibody [EPR13320-66]

Immunocytochemistry/ Immunofluorescence - Anti-kur antibody [EPR13320-66] (AB181173)

Immunoprecipitation - Anti-kur antibody [EPR13320-66] (AB181173)

Western blot - Anti-kur antibody [EPR13320-66] (AB181173)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise