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AB250399

Anti-kur antibody [EPR13320-66] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal kur antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C21orf48, C21orf59, CFAP298, Cilia- and flagella-associated protein 298, Protein kurly homolog

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-kur antibody [EPR13320-66] - BSA and Azide free (AB250399)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-kur antibody [EPR13320-66] - BSA and Azide free (AB250399)

This data was developed using ab181173, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of MCF7 cells (paraformaldehyde-fixed, 4%), labeling kur with ab181173 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Immunoprecipitation - Anti-kur antibody [EPR13320-66] - BSA and Azide free (AB250399)
  • IP

Supplier Data

Immunoprecipitation - Anti-kur antibody [EPR13320-66] - BSA and Azide free (AB250399)

This data was developed using ab181173, the same antibody clone in a different buffer formulation.

Western blot analysis of Jurkat cell lysate immunoprecipitated using ab181173 at 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody used at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-kur antibody [EPR13320-66] (<a href='/en-us/products/primary-antibodies/kur-antibody-epr13320-66-ab181173'>ab181173</a>)

Predicted band size: 33 kDa

false

Western blot - Anti-kur antibody [EPR13320-66] - BSA and Azide free (AB250399)
  • WB

Supplier Data

Western blot - Anti-kur antibody [EPR13320-66] - BSA and Azide free (AB250399)

This data was developed using ab181173, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-kur antibody [EPR13320-66] (<a href='/en-us/products/primary-antibodies/kur-antibody-epr13320-66-ab181173'>ab181173</a>) at 1/5000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13320-66

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250399 is the carrier-free version of ab181173.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kur also known as the voltage-gated potassium channel subunit Kv3.x is an integral membrane protein involved in potassium ion transport across cell membranes. With a molecular mass of approximately 85 kDa this channel exhibits a high expression level in central nervous system tissues particularly in neurons. It plays a critical role in modulating neuronal excitability and signal transmission by facilitating rapid repolarization of the action potential.
Biological function summary

The voltage-gated potassium channel Kv3.x influences neurotransmitter release and neuronal firing rate. This target does not function alone; rather it forms a part of a larger channel complex. Through these channels Kv3.x regulates the rapid spike frequency adaptation enabling neurons to maintain high-frequency firing without depolarization block. By contributing to electrical signaling precision it supports diverse neuronal circuit activities.

Pathways

The potassium channel Kv3.x integrates into the neuronal excitability pathway affecting both synaptic transmission and plasticity. It interacts with key proteins like Nav1.6 a voltage-gated sodium channel as they complement each other's roles in action potential propagation. Kv3.x also participates in the broader potassium ion homeostasis pathway which involves other ion channels and transporters to maintain neuronal ion balance and function.

Dysregulation of Kv3.x channels associates with neurological conditions such as epilepsy and schizophrenia. Altered expression or function of Kv3.x can lead to increased neuronal excitability contributing to seizure susceptibility in epilepsy. In schizophrenia abnormal Kv3.x activity could disrupt normal auditory processing potentially through interactions with proteins like NMDA receptors. These associations highlight the importance of Kv3.x in maintaining neural health and signaling fidelity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in motile cilium function, possibly by acting on outer dynein arm assembly (PubMed : 24094744). Seems to be important for initiation rather than maintenance of cilium motility (By similarity). Required for correct positioning of the cilium at the apical cell surface, suggesting an additional role in the planar cell polarity (PCP) pathway (By similarity). May suppress canonical Wnt signaling activity (By similarity).
See full target information CFAP298
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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