AB21089

Anti-Kv1.6/RCK2 antibody

(1 Review)

(2 Publications)

Rabbit Polyclonal Kv1.6/RCK2 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications.

View Alternative Names

Potassium voltage-gated channel subfamily A member 6, Voltage-gated potassium channel HBK2, Voltage-gated potassium channel subunit Kv1.6, KCNA6

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Kv1.6/RCK2 antibody (AB21089)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Kv1.6/RCK2 antibody (AB21089)

ICC/IF image of ab21089 stained human HEK (Human embryonic kidney) 293 cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab21089, 5μg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

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Western blot - Anti-Kv1.6/RCK2 antibody (AB21089)

ab21089 recognises a band corresponding to the predicted MW of Kv1.6/RCK2 in mouse brain lysate (lane 1). Antibody binding to this band may be blocked using the immunising peptide, ab23431 (lane 2). The antibody also recognises additional proteins. These may be degradation/cleavage products of Kv1.6/RCK2, or they may represent non-specific binding by the antibody.

All lanes:

Western blot - Anti-Kv1.6/RCK2 antibody (ab21089) at 1 µg/mL

Lane 1:

Mouse Brain Lysate at 20 µg

Lane 2:

Mouse Brain Lysate Kv1.6/RCK2 at 20 µg with Human Kv1.6/RCK2 peptide (<a href='/en-us/products/unavailable/human-kv16rck2-peptide-ab23431'>ab23431</a>)

Secondary

Lane 1:

Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution

Lane 2:

Alexa Fluor Goat polyclonal to Rabbit IgG

Predicted band size: 59 kDa

Observed band size: 35 kDa,39 kDa,45 kDa,58 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

In human HEK293 cells ab21089 recognised a band of ~58 kDa, the predicted MW of Kv1.6/RCK2 (data not shown). This band was not blocked by the immunising peptide. Additional bands of 100 kDa, 25 kDa and 20 kDa were also recognised.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kv1.6 also known as RCK2 or KCNA6 is a voltage-gated potassium channel with a molecular weight of approximately 58 kDa. It plays a role in facilitating the movement of potassium ions across the cell membrane contributing to the restoration of resting membrane potential following action potentials. Kv1.6 is found in a variety of tissues including the brain heart and skeletal muscles. It participates in forming a tetrameric structure typical for voltage-gated potassium channels working alongside other related subunits to exhibit the complete functional properties.

Biological function summary

Kv1.6 contributes to the stabilization of electrical signaling in neurons and muscle cells. It participates in shaping action potentials and regulating neuronal excitability. Kv1.6 forms part of the larger voltage-gated potassium channel complex which modulates membrane potential dynamics and excitability helping ensure proper nervous system functionality and muscle responsiveness.

Pathways

Kv1.6 is involved in the regulation of ion homeostasis and the maintenance of neuronal action potentials. It participates in influencing the duration and frequency of action potentials in neurons by cooperating with other voltage-gated channels such as Kv1.1 and Kv1.2. Kv1.6's activity indirectly affects pathways like the MAPK signaling pathway by participating in the overall electrical activity that may influence signaling cascades critical for cell survival and differentiation.

Kv1.6 has been linked to conditions like epilepsy and hypertension. Alterations in the function or expression of Kv1.6 can disrupt normal neuronal excitability contributing to epileptic phenotypes where excessive neuronal firing occurs. Moreover in hypertension aberrant Kv1.6 activity impacts vascular smooth muscle excitability and contractility. Its relation to proteins such as Kv1.1 in epilepsy highlights its collaborative role in ensuring proper neuronal function while interactions with other ion channels in vascular tissues underline its potential involvement in hypertension.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient (PubMed : 14575698, PubMed : 2347305). The channel alternates between opened and closed conformations in response to the voltage difference across the membrane (PubMed : 14575698, PubMed : 2347305). Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNA1, KCNA2, KCNA4, KCNA6, and possibly other family members as well; channel properties depend on the type of alpha subunits that are part of the channel (By similarity). Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation (By similarity). Homotetrameric channels display rapid activation and slow inactivation (PubMed : 2347305).

See full target information KCNA6

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroscience research 100:506-521 PubMed34935172

2021

Adult-onset hypothyroidism causes mechanical hypersensitivity due to peripheral nerve hyperexcitability based on voltage-gated potassium channel downregulation in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Machiko Suda,Yusuke Takatsuru,Izuki Amano,Shogo Haraguchi,Noriyuki Koibuchi

Oncogene 29:2499-508 PubMed20173781

2010

Targeting the FANCJ-BRCA1 interaction promotes a switch from recombination to poleta-dependent bypass.

Applications

Unspecified application

Species

Unspecified reactive species

J Xie,R Litman,S Wang,M Peng,S Guillemette,T Rooney,S B Cantor

View all publications

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Product promise

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Anti-Kv1.6/RCK2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Kv1.6/RCK2 antibody (AB21089)

Western blot - Anti-Kv1.6/RCK2 antibody (AB21089)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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