Anti-Kv4.2/KCND2 antibody [EPR26384-89] - BSA and Azide free

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Kv4.2/KCND2 antibody [EPR26384-89] - BSA and Azide free (AB307711)

This data was developed using ab307710, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Rat cerebellum tissue staining PKC gamma with ab317315 at a 1 : 5000 (0.1 µg/ml) dilution, KCNA2 with ab316324 at 1 : 4000 (0.124 µg/ml) dilution and KCND2 with ab307710 at a 1 : 2000 (0.266 µg/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-PKC gamma (green; Opal^™520), anti-KCNA2 (magenta; Opal^™690) and anti-KCND2 (gray; Opal^™570) on rat cerebellum.
Panel B : anti-PKC gamma staining the Purkinje cells and neuropil in rat cerebellum.
Panel C : anti-KCNA2 staining the basket cells in rat cerebellum.
Panel D : anti-KCND2 staining the granule cell layer in rat cerebellum.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab317315, ab313624 and ab307710 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.2/KCND2 antibody [EPR26384-89] - BSA and Azide free (AB307711)

This data was developed using ab307710, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Kv4.2/KCND2 with ab307710 at 1/2000 (0.266 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on mouse liver (PMID : 16293790). The section was incubated with ab307710 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Kv4.2/KCND2 antibody [EPR26384-89] - BSA and Azide free (AB307711)

This data was developed using ab307710, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse dorsal root ganglion (fresh) tissue labeling Kv4.2/KCND2 with ab307710 at 1/100 (5.32 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Panel A : merged staining of anti-Kv4.2 (ab307710, green) and anti-NeuN (ab190565, red) on mouse dorsal root ganglion.Panel B : anti-Kv4.2 stained on the mouse dorsal root ganglion.Panel C : anti-NeuN stained in neurons of mouse dorsal root ganglion.Negative control : dorsal root ganglion (PMID : 19668710).The nuclear counterstain was DAPI (Blue). The section was incubated in two rounds of staining : in the order of ab307710 and ab190565 for 1 hr at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Kv4.2/KCND2 antibody [EPR26384-89] - BSA and Azide free (AB307711)

This data was developed using ab307710, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling Kv4.2/KCND2 with ab307710 at 1/100 (5.32 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control : confocal image showing no staining on rat liver (PMID : 10729221). The nuclear counterstain was DAPI (Blue). The section was incubated with ab307710 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Kv4.2/KCND2 antibody [EPR26384-89] - BSA and Azide free (AB307711)

This data was developed using ab307710, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling Kv4.2/KCND2 with ab307710 at 1/100 (5.32 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control : confocal image showing no staining on mouse liver (PMID : 10729221). The nuclear counterstain was DAPI (Blue). The section was incubated with ab307710 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Kv4.2/KCND2 antibody [EPR26384-89] - BSA and Azide free (AB307711)

This data was developed using ab307710, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat dorsal root ganglion (fresh) tissue labeling Kv4.2/KCND2 with ab307710 at 1/100 (5.32 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Panel A : merged staining of anti-Kv4.2 (ab307710, green) and anti-NeuN (ab190565, red) on rat dorsal root ganglion.Panel B : anti-Kv4.2 stained on the rat dorsal root ganglion.Panel C : anti-NeuN stained in neurons of rat dorsal root ganglion.Negative control : dorsal root ganglion (PMID : 19668710).The nuclear counterstain was DAPI (Blue). The section was incubated in two rounds of staining : in the order of ab307710 and ab190565 for 1 hr at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.2/KCND2 antibody [EPR26384-89] - BSA and Azide free (AB307711)

This data was developed using ab307710, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling Kv4.2/KCND2 with ab307710 at 1/2000 (0.266 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on rat cerebellum (PMID : 32899153). The section was incubated with ab307710 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.2/KCND2 antibody [EPR26384-89] - BSA and Azide free (AB307711)

This data was developed using ab307710, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling Kv4.2/KCND2 with ab307710 at 1/2000 (0.266 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on mouse cerebellum (PMID : 17122039). The section was incubated with ab307710 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Kv4.2/KCND2 antibody [EPR26384-89] - BSA and Azide free (AB307711)

This data was developed using ab307710, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections of Rat cerebellum tissue staining with ab317831 at 1/2000 dilution, ab313624 at 1/4000 dilution, ab307710 at 1/2000 dilution. Secondary used was Opal Polymer HRP Ms + Rb. Panel A : merged staining of anti-Ppp1r17 (magenta; Opal™690), anti-KCNA2 (green; Opal™520) and anti-KCND2 (gray; Opal™570) on rat cerebellum. Panel B : anti-Ppp1r17 staining the Purkinje cells in rat cerebellum. Panel C : anti-KCNA2 staining the basket cells in rat cerebellum. Panel D : anti-KCND2 staining the granule cell layer in rat cerebellum. Nuclear DNA was labeled with DAPI (shown in blue). The section was incubated in three rounds of staining : in the order of ab317831, ab313624 and ab307710 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Kv4.2/KCND2 antibody [EPR26384-89] - BSA and Azide free (AB307711)

This data was developed using ab307710, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse cerebellum tissue staining PKC gamma with ab317315 at a 1 : 5000 (0.1 µg/ml) dilution, KCNA2 with ab316324 at 1 : 4000 (0.124 µg/ml) dilution and KCND2 with ab307710 at a 1 : 2000 (0.266 µg/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-PKC gamma (green; Opal^™520), anti-KCNA2 (magenta; Opal^™690) and anti-KCND2 (gray; Opal^™570) on mouse cerebellum.
Panel B : anti-PKC gamma staining the Purkinje cells and neuropil in mouse cerebellum.
Panel C : anti-KCNA2 staining the basket cells in mouse cerebellum.
Panel D : anti-KCND2 staining the granule cell layer in mouse cerebellum.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab317315, ab313624 and ab307710 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Kv4.2/KCND2 antibody [EPR26384-89] - BSA and Azide free (AB307711)

This data was developed using ab307710, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections of Mouse cerebellum tissue staining with ab317831 at 1/2000 dilution, ab313624 at 1/4000 dilution, ab307710 at 1/2000 dilution. Secondary used was Opal Polymer HRP Ms + Rb. Panel A : merged staining of anti-Ppp1r17 (magenta; Opal™690), anti-KCNA2 (green; Opal™520) and anti-KCND2 (gray; Opal™570) on mouse cerebellum. Panel B : anti-Ppp1r17 staining the Purkinje cells in mouse cerebellum. Panel C : anti-KCNA2 staining the basket cells in mouse cerebellum. Panel D : anti-KCND2 staining the granule cell layer in mouse cerebellum. Nuclear DNA was labeled with DAPI (shown in blue). The section was incubated in three rounds of staining : in the order of ab317831, ab313624 and ab307710 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.2/KCND2 antibody [EPR26384-89] - BSA and Azide free (AB307711)

This data was developed using ab307710, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Kv4.2/KCND2 with ab307710 at 1/2000 (0.266 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on rat liver. The section was incubated with ab307710 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Kv4.2/KCND2 antibody [EPR26384-89] - BSA and Azide free (AB307711)

This data was developed using ab307710, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Rat primary neuron cells cells labelling Kv4.2/KCND2 with ab307710 at 1/50 dilution (1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Kv4.2/KCND2 antibody [EPR26384-89] - BSA and Azide free (AB307711)

This data was developed using ab307710, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum (fresh) tissue labeling Kv4.2/KCND2 with ab307710 at 1/100 (5.32 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Panel A : merged staining of anti-Kv4.2 (ab307710, green) and anti-NeuN (ab190565, red) on rat cerebellum.Panel B : anti-Kv4.2 stained on the rat cerebellum.Panel C : anti-NeuN stained in neurons of rat cerebellum.The nuclear counterstain was DAPI (Blue). The section was incubated in two rounds of staining : in the order of ab307710 and ab190565 for 1 hr at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Kv4.2/KCND2 antibody [EPR26384-89] - BSA and Azide free (AB307711)

This data was developed using ab307710, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum (fresh) tissue labeling Kv4.2/KCND2 with ab307710 at 1/100 (5.32 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Panel A : merged staining of anti-Kv4.2 (ab307710, green) and anti-NeuN (ab190565, red) on mouse cerebellum.Panel B : anti-Kv4.2 stained on the mouse cerebellum.Panel C : anti-NeuN stained in neurons of mouse cerebellum.The nuclear counterstain was DAPI (Blue). The section was incubated in two rounds of staining : in the order of ab307710 and ab190565 for 1 hr at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Kv4.2/KCND2 antibody [EPR26384-89] - BSA and Azide free (AB307711)

This data was developed using ab307710, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cells cells labelling Kv4.2/KCND2 with ab307710 at 1/50 dilution (1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

• IP

Supplier Data

Immunoprecipitation - Anti-Kv4.2/KCND2 antibody [EPR26384-89] - BSA and Azide free (AB307711)

This data was developed using ab307710, the same antibody clone in a different buffer formulation. Kv4.2/KCND2 was immunoprecipitated from 0.35 mg Rat cerebellum tissue lysate with ab307710 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307710 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Rat cerebellum tissue lysate Lane 2 : ab307710 IP in Rat cerebellum tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307710 in rat cerebellum tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 8 seconds

All lanes:

Immunoprecipitation - Anti-Kv4.2/KCND2 antibody [EPR26384-89] (<a href='/en-us/products/primary-antibodies/kv42-kcnd2-antibody-epr26384-89-ab307710'>ab307710</a>) at 1/30 dilution

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 8s

• IP

Supplier Data

Immunoprecipitation - Anti-Kv4.2/KCND2 antibody [EPR26384-89] - BSA and Azide free (AB307711)

This data was developed using ab307710, the same antibody clone in a different buffer formulation. Kv4.2/KCND2 was immunoprecipitated from 0.35 mg Mouse cerebellum tissue lysate with ab307710 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307710 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Mouse cerebellum tissue lysate Lane 2 : ab307710 IP in Mouse cerebellum tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307710 in mouse cerebellum tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 8 seconds

All lanes:

Immunoprecipitation - Anti-Kv4.2/KCND2 antibody [EPR26384-89] (<a href='/en-us/products/primary-antibodies/kv42-kcnd2-antibody-epr26384-89-ab307710'>ab307710</a>) at 1/30 dilution

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 8s

• WB

Supplier Data

Western blot - Anti-Kv4.2/KCND2 antibody [EPR26384-89] - BSA and Azide free (AB307711)

This data was developed using ab307710, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : liver(Human Protein Atlas Database), bladder (PMID : 12575952). The molecular weight observed is consistent with what has been described in the literature (PMID : 15454437). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 26 seconds

All lanes:

Western blot - Anti-Kv4.2/KCND2 antibody [EPR26384-89] (<a href='/en-us/products/primary-antibodies/kv42-kcnd2-antibody-epr26384-89-ab307710'>ab307710</a>) at 1/1000 dilution

Lane 1:

Mouse cerebellum tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Mouse bladder tissue lysate at 20 µg

Lane 4:

Rat cerebellum tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 70 kDa,250 kDa,300 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-Kv4.2/KCND2 antibody [EPR26384-89] - BSA and Azide free (AB307711)

This data was developed using ab307710, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody does not cross-react with mouse KCND1 and KCND3. In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. Exposure time : 10 seconds

All lanes:

Western blot - Anti-Kv4.2/KCND2 antibody [EPR26384-89] (<a href='/en-us/products/primary-antibodies/kv42-kcnd2-antibody-epr26384-89-ab307710'>ab307710</a>) at 1/1000 dilution

Lane 1:

His-tagged mouse KCND1 fragment at 10 ng

Lane 2:

His-tagged mouse KCND2 fragment at 10 ng

Lane 3:

His-tagged mouse KCND3 fragment at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 26 kDa

false

Exposure time: 10s